High Quality RNA Isolation from Leaf, Shell, Root Tissues and Callus of Hazelnut (Corylus avellana L.)

Journal of Nuts

مقاله 1، دوره 08، شماره 02، اسفند 2017، صفحه 89-95   

شناسه دیجیتال (DOI): 10.22034/jon.2017.536237

نویسندگان

Hossein Khosravi¹؛ Ali Deljou²؛ Sajad Rashidi Monfared³

¹Department of Plant Biotechnology, faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

²Department of Plant Biotechnology, faculty of Agriculture, Bu-Ali Sina University, Hamedan

³Department of Biotechnology, faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran

چکیده

Extraction of high quality RNA is a critical step in molecular genetics studies. Hazelnut is one of the most important nuts plants in the world. The presence of the taxol and other taxanes in hazelnut plant necessitates explaining their biosynthesis pathway and identifying the candidate genes. Therefore, an easy and practical method is necessary for RNA extraction from hazelnuts. Hazelnut has high levels of phenolic compounds. High amounts of polyphenolic and polysaccharide compounds in plants could be causing problems in RNA extraction procedures.  To avoid these problems, a simple and efficient method can be used based on cetyltrimethylammonium bromide (CTAB) extraction buffer and lithium chloride for extraction of high quality RNA from different parts of hazelnut plant. Using this method, a high-quality RNA sample (light absorbed in the A260/A280 was 2.04

کلیدواژه‌ها

CTAB extraction؛ Hazelnut؛ RNA extraction؛ Taxol

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