The Study of Morphological Traits and Identification of Self-incompatibility Alleles in Almond Cultivars and Genotypes

Journal of Nuts

مقاله 6، دوره 08، شماره 02، اسفند 2017، صفحه 137-150   

شناسه دیجیتال (DOI): 10.22034/jon.2017.536244

نویسنده

Mousa Rasouli

Department of Horticulture and Landscape, Faculty of Agriculture, Malayer University, P.O. Box 65719-95863 Malayer, Iran

چکیده

The evaluation of an almond collection using morphological variables and identification of self-incompatibility genotype  is useful for selecting pollinizers and for the design of crossing in almond breeding programs. In this study, important morphological traits and self-incompatibilities in 71 almond cultivars and genotypes were studied. Simple and multiplex specific PCR analyses were used in order to identify self-incompatibility alleles. Based on the results, cultivars and genotypes including ‘Dir Ras–e-Savojbolagh’, ‘D-124’, ‘D-99’, ‘Shahrood 12’, ‘Tuono’, ‘Nonpareil’, ‘Price’, ‘Mirpanj-e-Tehran’, ‘Pakotahe-e- Taleghan’, ‘V-13-34’, ‘V-16-8, ‘V-11-10’, ‘Zarghan 10’, ‘Uromiyeh 68’, ‘Barg dorosht-e-Hamedan’ and ‘Yazd 60’ were late flowering and had the highest quality of nut and kernel characters. The result of the PCR method using combined primers AS1II and AmyC5R showed amplification of ten self-incompatibility alleles (S₁, S₂, S₃, S₅, S₆, S₇, S₈, S₁₀, S₁₂,and S unknown allele) and three S_falleles. Moreover, S₁ had the highest frequencies in comparison with other known S-alleles. Also, unknown alleles with different sizes were detected and 58 new bands were found in some cultivars. 

کلیدواژه‌ها

AS1II؛ AmyC5R؛ Incompatibility؛ Kernel؛ Prunus dulcis؛ S-allele؛ Specific PCR

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