بررسی سرواپیدمیولوژیکی ویروس بلوتانگ در بزهای استان چهار محال وبختیاری

نشریه علمی میکروبیولوژی دامپزشکی

مقاله 4، دوره 13، شماره 1 - شماره پیاپی 34، شهریور 1396، صفحه 33-43   

نوع مقاله: مقاله پژوهشی (اصیل)

نویسندگان

وحید نعمان^{* 1}؛ هدی ارزانی²

¹اصفهان بلوار کشاورز، شهرک امیریه، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی اصفهان، بخش تحقیقات دامپزشکی

²دانش آموخته کارشناسی ارشد زیست شناسی گرایش میکروبیولو‍ژی، دانشگاه آزاد اسلامی واحد نایین

چکیده

در این مطالعه جهت تشخیص آنتی بادی ویروس بلوتانگ در بز استان چهارمحال و بختیاری تحقیقی در سال 1394در دو منطقه اکولوژیکی انجام گرفت. نمونه های خون بصورت تصادفی از بزهای دو منطقه اخذ شد. برای بررسی آنتی بادی اختصاصی بلوتانگ نمونه های سرمی با آزمون الیزای رقابتی مورد بررسی قرار گرفتند. از 1350 نمونه سرم بز 48/57% مثبت ارزیابی شدند. دربررسی شیوع سرمی ویروس بلوتانگ در جمعیت بز از520 نمونه سرم منطقه جلگه ای و830 نمونه سرم منطقه کوهستانی به ترتیب 04/79% و98/43% نمونه مثبت ارزیابی شدند. اختلاف معناداری از نظر موارد مثبت در دو منطقه  مشاهده شد (P

کلیدواژه‌ها

بلوتانگ؛ سرواپیدمیولوژی؛ الیزای رقابتی؛ بز؛ استان چهارمحال و بختیاری؛ ایران

مراجع

1-Afshar, A.  and  Kayvanfar, H. (1974).  Occurrence of precipitating antibodies to bluetongue virus in sera of farm animals in Iran. Veterinary Record 94: 233-235.

2-Anthony,S. Jones ,H . Darpel, K.E . Elliott, H . Maan, S. Samuel, A . Mellor, PS. Mertens, PP.(2007). A duplex RT-PCR assay for detection of genome segment 7 (VP7) from 24 BTV serotypes. Journal of Virological Methods 141:188-197.

3-Bastawecy, I.M. Fayoumi, EL. Competitive, M.M. (2006). ELISA test for diagnosis of Bluetongue in Egypthttp.://www. en.engormix.com/ MA- dairy-cattle/ articles/ competitive-elisa-test-diagnosist248/ p0.htm. (Accessed 2 may2015(

4-Biteau-Coroller,F. Gerbier, G. , Stark, K.D.C., Grillet,C. (2006). Performance evaluation of competitive ELISA test used for bluetongue antibody detection in France, a recent infected area. Veterinary Microbiology 118:57-66.

5-Chand, K. Biswas SK. De A. Sing B. Mondal, B.(2009).  A polyclonal antibody-based sandwich ELISA for the detection of bluetongue virus in cell culture and blood of sheep infected experimentally. Journal of Virological Methods 160: 189-192.

6-Chauhan H.C, kher, H.N.chandel B.S, Dadawala, A I. Jain, L. Agrawal, S M .Bhadaniya,A. (2010). valuation of group specific nested  pcr for detection of bluetongue virus. veterinary world department 2:179-182

7-Coetzee,p.Stokstad, M.Venter, H. E. Myrmel, M . Van Vuuren, M. (2012).   Bluetongue: a historical and epidemiological perspective with the emphasis on South Africa. Virology Journal  9: 198.

8-Formenty, P.; Domenech, J.; Lauginie, F.; OuattaraM.; Diawara, S.; Raath, J.P. Grobler, D. Leforban, Y. Angba, A. (1994). Epidemiologic  study  of  bluetongue  in  sheep, cattle and different species of wild animals in theIvory  Coast.  Revue Scientifique  et  Technique Journal  13: 737-751

9-García, I. Napp, S. Casal, J. Perea, A. Allepuz, A. Alba , A. Carbonero, A. Arenas A. (2009).   Bluetongue epidemiology  in  wild  ruminants  from  Southern Spain.  European  Journal  of  Wildlife  Research 55: 173–178.

10- Gür, A.(2008). serologic investigation of blue tongue virus (BTV) in cattle, sheep and gazella subgutturosa subgutturosa in southeastern turkey. Tropical Animal Health and Production 40:217-21 ..

11-Hasanpour, A. Mosakhani, F. Mirzaii, H.Mostofi, S . (2008).Seroprevalence of bluetongue virus Infection in sheep in East- Azerbaijan Province in Iran. Research Journal of Biological Sciences. 3:1265-1270

12- khezri, M., Bakhshesh, M. (2014). Investigation of Bluetongue in Sheep in Western Iran with an Overview of Infection Since 1972.Journal of Scientific Research and Reports. 3:787-798

13-Khanbabaie, H. Fakour, SH. Khezri, M. Mohamadian, B. Farokhzad, B. )2012(.Serological survey of bluetongue disease insheep of sanandaj city by Elisa. journal of veterinary medicine 5 :11 -18.

14-Khezri, M. and Azimi, S.M. (2012).  Investigation of  bluetongue  virus  in  Kurdish  sheep  in Kurdistan  province  of  Iran. African  Journal  of Microbiology Research 6: 6496-6501.

15-Khezri, M. Azimi, SM. ( 2013).  Seroprevalence of bluetongue disease in sheep in west and northwest provinces of Iran. Veterinary Research Forum 4 :195 – 198

16-Khezri, M. and Azimi, S.M. (2013).  Epidemiological investigation of bluetongue virus antibodies in sheep in Iran,Veterinary World 6:122-125.

17-Maclachlan, NJ. Osburn, BI. ( 2006). Impact of bluetongue virus infection on the international movement and trade of ruminants. American Veterinary Medical Association 228: 1346-1349

18-Maclachlan, NJ. (2011).  Bluetongue:  history, global  epidemiology,  and  Pathogenesis. PreventiveVeterinary Medicine 102: 107-11

19-Mahzounieh, M.R. Golestanfar, A. Salimi, M. Jamali Torkabad, M. (2013). Detection of bluetongue virus in aborted lamb fetuses in Chaharmahal va Bakhtiari and Isfahan provinces, by RT-PCR method August. Veterinary Journal  104: 17-21

20-Mohammadi, A., Tanzifi, P. and Nemati, Y. (2012).  Seroepidemiology of bluetongue disease and risk factors in small ruminants of Shiraz suburb, Fars province,  Iran.  Tropical  Biomedicine29: 632–637.

21-Mozaffari.A,  and Khalili, M. Yahyazadeh, F. (2012) . A serological investogation of bluetongue virus in cattle of South-east iran .Veterinaria Italiana. 48 :41-44.

22-Mozaffari,.AA، Khalili M .Sabahi، S. )2014.( High seroprevalence of bluetongue virus antibodies in goats in southeast Iran. Asian Pacific Journal of Tropical Biomedicine4: 275–278.

23-Najarnezhad ,V. Rajae, M. ( 2013). Seroepidemiology of bluetongue disease in small ruminants of north-east of Iran. Asian Pacific Journal of Tropical Biomedicine 3: 492–495

24-Noaman , V. Kargar-Moakhar, R. Shahmoradi, A.H. Heidari, M.R. Tabatabaei, J. Nabinejad A.R. . (2008).   Use of competitive ELISA for serological detection of bluetongue virus antibody in sheep and goats of Isfahan Province, Iran. Pajouhesh-va-sazandegi . in animal and fisheries sciences 21: 39-48.

25-Noaman,V. Shirvani ,E. Hosseini, SM. Shahmoradied, AH.Heidari, MR. Raiszadeh, H. Kamalzadeh, M. Bahreyari, M. ( 2013). Serological surveillance of bluetongue virus in cattle in central Iran.Veterinaria Italiana  49: 141-144.

26-Noroozikia, Sh. Pourmahdi Borujeni, M. Haji Hajikolaei, M.R .Seifi, M.R. (2014).  Seroepidemiological survey of bluetongue disease in sheep in Khuzestan province " Iranian Veterinary Journal Spring. 10: 103-111

27-Smiriti, S. Shringi, B.N .(2005) . Comparative efficacy of standard AGID, CCIE and competitive ELISA for detecting Bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan. Indian. Journal of Veterinary Science  6:77-79.

28-Subhadra,S. Kumar,S. Suryanarayana,V. ( 2014).  Sreenivasulu,DComparison of bluetongue virus detection and quantitation methods in south India. The Journal of Infection in Developing Countries 8:1307-1312

29-Toussaint, J.F.; Sailleau, C.; Mast  J.; Houdart, P.; Czaplicki, G.; Demeestere, L. VandenBussche,F W van Dessel,W. Goris,N. Bréard,E. Bounaadja,L. Thiry,E. Zientara,S and de Clercq ,K. Bluetongue  in  Belgium (2007) Emerging  Infectious Diseases 13: 614- 616.

30-Worwa, G., Chaignat, V., Feldmann, J. and Thur, B.) 2013(.Detection of neutralizing antibodies against bluetongue virus serotype . Journal of virological methods 188: 168-74

31-Yin ,HQ. Zhang, H. Shi, LJ .Yang, S .Zhang, GP.  Wang, SQ. Zhang, JG .( 2010 ). Detection and quantitation of bluetongue virus serotypes by a TaqMan probe-based real-time RT-PCR and differentiation fromepizootic hemorrhagic disease virus.Journal of Virological Methods  168:237-41

32-Yousef, M.  Al-Eesa, A.A. Al-Blowi,M.H. (2012). High seroprevalence of bluetongue virus antibodies in Sheep, Goats, Cattle and Camel in different districts of Saudi Arabia. Veterinary World. 5:389-393.