Biological Control of African Violets Root-Knot Disease by the Used of Extracellular Protease Bacillus

Journal of Ornamental Plants

مقاله 5، دوره 7، شماره 4، اسفند 2017، صفحه 247-255   

نوع مقاله: Research Paper

نویسندگان

Hadi Rahanandeh^{* 1}؛ Mahsa Moshayedi²

¹Member of Department of Plant Pathology, Faculty of Agriculture, Rasht Branch, Islamic Azad University, Rasht, Iran

²Young Researchers and Elite Club, Rasht Branch, Islamic Azad University

چکیده

The present study explored the efficacy of Bacillus spp. and protease production for biocontrol of the root-knot nematode Meloidogyne javanica in African violet media. Among 100 bacterial isolates from various soils, the highest nematode mortality was observed for treatments with isolate GM-18, which was identified as Bacillus subtilis based on cultural and morphological characteristics and 16S rDNA sequencing analyses. The Bacillus spp. showed nematicidal activity 74.01 to 28.33. Strain GM18 showed the most inhibitor zone in medium containing casein (13 mm) and nematicidal activity in our tests targeting Meloidogyne javanica. The effect of different variables such as time (1-24 h), pH (7- 8.5), and temperature (25- 37 °C) on the protease production was checked. The maximum enzyme production was noted when B. subtilis GM18 was grown on mineral medium and incubated at 31°C for 12 h with initial pH = 8. Three protease inhibitors that had the greatest influence on EDTA were studied. Protease produced by B. subtilis GM18belonged to different groups.

کلیدواژه‌ها

African violet media؛ <i>Bacillus</i>؛ Biological control؛ Root knot disease

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