Effect of Gradual and Shock Chilling Stress on Abscisic Acid, Soluble Sugars and Antioxidant Enzymes Changes in ‘Sultana’ Grapevine.

Iranian Journal of Plant Physiology

مقاله 8، دوره 7، شماره 4، آبان 2017، صفحه 2211-2224   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2017.537989

نویسندگان

Shima Sadat Beheshti Rooy¹؛ Ghasem Hosseini Salekdeh²؛ Mehdi Ghabooli³؛ Mansour Gholami⁴؛ Ehsan Mohsenifard⁵؛ Rouhollah Karimi^{* 6}

¹Grapevine Production and Genetic Improvement Department, Iranian Grape and Raisin Institute, Malayer University, Iran

²2. Department of Systems Biology, Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education, and Extension Organization, Karaj, Iran

³Department of Agronomy, Faculty of Agriculture, Malayer University, Iran

⁴4. Department of Horticulture, Faculty of Agriculture, Bu-Ali Sina University, Iran

⁵Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Iran

⁶Department of Landscape Engineering, Faculty of Agriculture, Malayer University, Iran

چکیده

 
Plants may show different behaviours during exposure to low temperature stress through various mechanisms. In this study, the chilling responses of ‘Sultana’ grapevine were investigated during a gradual chilling (2 °C/h) and also during a shock chilling (5 °C/h) from 24 °C to 4 °C. After artificial chilling stress, electrolyte leakage, lipid peroxidation, hydrogen peroxide production and antioxidant enzymes activities were measured in all plants leaves. Moreover, the potential of plant to accumulate abscisic acid and osmoregulants were compared under both gradual and shock chilling stresses. Based on the results, a significant difference was found between the electrolyte leakage, lipid peroxidation and hydrogen peroxide content of plants under two chilling regimes. These indices were found to be higher in shock chilling stressed plants compared with gradual chilling stressed vines because of the lower catalase, peroxidase and ascorbate peroxidase activities. Moreover, gradual chilling stressed vines exhibited higher accumulation of abscisic acid, proline and total phenolic compounds. The chlorophyll degradation and relative water content were lower in gradual cold stressed plants. Glucose and fructose, unlike sucrose showed higher concentration in gradual cold stressed vines. Totally, under gradual chilling stress grapevine plants showed better cold acclimation by lower oxidative stress and higher accumulation of osmoregulants in compared with shock chilling stressed vines.
 

کلیدواژه‌ها

Chilling regimes؛ Cold stress؛ grapevine؛ Metabolic changes