بررسی اثر عصاره روغنی تخم خرفه (Portulaca oleracea) بر شاخص‌های مقاومت انسولینی در موش سوری

آسیب‌شناسی درمانگاهی دامپزشکی

مقاله 2، دوره 11، 4 (44) زمستان، بهمن 1396، صفحه 313-323   

نوع مقاله: مقاله علمی پژوهشی

نویسندگان

علی کارگری رضاپور^{* 1}؛ رعنا وطن دوست²

¹استادیار کلینیکال پاتولوژی دامپزشکی، گروه علوم دامی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

²دانش‌آموخته کارشناسی ارشد زیست شناسی–بیوشیمی، گروه زیست شناسی، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

چکیده

استفاده از داروهای با منشأ طبیعی برای درمان دیابت مورد توجه قرار گرفته ­است. خرفه با نام علمی Portulaca oleracea از جمله گیاهان دارویی مهم بومی ایران است. هدف از مطالعه حاضر، بررسی اثر عصاره­ روغنی تخم خرفه بر شاخص­های مقاومت انسولینی در موش سوری می­باشد.در این مطالعه 30 سر موش سوری به 5 گروه 6 تایی تقسیم­بندی شدند: 1- تیمار شاهد منفی (غیردیابتی)، 2- تیمار شاهد مثبت (دیابتی)، 3- تیمار شاهد عصاره­­­­­­­­­­­­­ خرفه غیردیابتی، 4- تیمار دیابتی با عصاره­ تخم خرفه (دوز mg/kg150) و 5- تیمار دیابتی با عصاره­ تخم خرفه (دوز mg/kg 300). تجویز عصاره خرفه از ابتدای آزمایش به­مدت یک ماه در گروه­های مربوطه (3 تا 5) انجام شد و پس از این مدت، القای دیابت با تزریق استرپتوزوتوسین به گروه­های 2، 4 و 5 صورت گرفت و پس از 2 ماه، از موش­ها خون­گیری شده و شاخص­های مورد نظر اندازه­گیری شد و مورد تحلیل آماری قرار گرفت. کل دوره آزمایش سه ماه بود.عصاره­ روغنی تخم خرفه تاثیر کاهنده­ معنی­داری بر گلوکز سرم خون در گروه­های دیابتی نسبت به شاهد دیابتی مثبت داشت (05/0p<). همچنین، غظت انسولین سرمی به­صورت معنی­داری در گروه­های 3، 4 و 5 که عصاره خرفه دریافت کرده بودند، افزایش یافت (05/0p<)، ولی در پروفایل­ لیپیدی و شاخص­های HOMA-IR، QUICKI و اندیس آتروژنیک تغییرات معنی­داری دیده نشد. نتایج مطالعه نشان داد عصاره تخم خرفه موجب کاهش گلوکز سرمی در موش­های دیابتی شده و دارای اثر انسولینوگوگ می­باشد.

کلیدواژه‌ها

تخم خرفه؛ دیابت؛ عصاره گیاهی؛ موش سوری

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