Influence of Defoliate Pathotype of Verticillium dahliae on Some Physiological and Biochemical Characteristics of Chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitam)

Journal of Ornamental Plants

مقاله 3، دوره 8، شماره 1، خرداد 2018، صفحه 23-33   

نوع مقاله: Research Paper

نویسندگان

seyed Javad Sanei^{* 1}؛ Seyed Esmael Razavi²

¹Plant protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

²Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

چکیده

Verticillium dahliae Kleb.is a soilborne pathogen that cause vascular wilt in chrysanthemumplant (Dendranthema grandiflorum (Ramat.) Kitam). The objective of this research was to study the levels of some organic solutes, such as total protein, total soluble sugars, starch and proline, relative water content, RNA level, malondialdehyde and H₂O₂ contents in leaves of chrysanthemuminoculated with V. dahliae. Changes of these parameters were measured on 0, 10, 20, 30 and 40 days after inoculation by spectrophotometric analysis. No changes in relative water content, RNA, and protein levels and a slight decrease in chlorophyll level were detected within infected leaf tissue before the appearance of visible wilt. The decrease in relative water content coincided with a sharp build up of proline and total soluble sugars in leaves. The leaf starch and protein levels gradually declined in both healthy and infected plants during the time course of the experiment. However, the decrease was more pronounced in infected plants from the third week after inoculation. A high negative correlation was observed between total soluble sugars and starch contents in leaves of diseased plants (r= 0.764, P<0.001). Changes in malondialdehyde and H₂O₂ concentration occurred in infected plants between 30-40 days after inoculation, while they did not change in the leaves of control plants. These data suggest the psssible role of senescence during the development of Verticillium wilt syndrome in chrysanthemum.

کلیدواژه‌ها

Antioxidant enzymes؛ reactive oxygen species؛ <i>Verticillium dahliae</i>؛ Wilt

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