Phytochemicals in leaves and extracts of the variety “Plovdiv 7” of Bulgarian oriental tobacco (Nicotiana tabacum L.)

Trends in Phytochemical Research

مقاله 4، دوره 2، شماره 1، خرداد 2018، صفحه 27-36   

نوع مقاله: Original Article

نویسندگان

Venelina Popova^{* 1}؛ Tanya Ivanova¹؛ Albena Stoyanova¹؛ Vasil Georgiev²؛ Tsveta Hristeva³؛ Violeta Nikolova³؛ Margarita Docheva³؛ Nikolay Nikolov³؛ Stanka Damianova⁴

¹University of Food Technologies, Department of Tobacco, Sugar, Vegetable and Essential Oils, Plovdiv, Bulgaria

²Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Sofia, Bulgaria

³Tobacco and Tobacco Products Institute, Markovo, Bulgaria

⁴Angel Kanchev University of Ruse, Razgrad Branch, Razgrad, Bulgaria

چکیده

This study aims to identify and analyze the non-volatile fraction (chemicals, polyphenols, phenolic acids, flavonoids, and triterpenes) and volatile fraction (essential oil and crude extracted aroma fractions) present in the leaves of “Plovdiv 7” oriental tobacco variety (N. tabacum L.). The results show that the nicotine content in leaves reaches 2.3% (DW) and chemicals such as betulin (2340.7 g/g), carnosic acid (845.2 µg/g) and ursolic acid (596.0 µg/g) (595.95 g/g ), are also present. In terms of free phenolic acids, chlorogenic acid (2545.0 g/g), ferulic acid (1561.8 g/g), and vanillic acid (4461.9 g/g) are found to be dominant. The flavonoid profile is dominated by myricetin (134.2 g/g), quercetin (334.1 g/g), apigenin (493.0 g/g) and luteolin (445.6 g/g). In the volatile fraction, nineteen volatile components were identified (92.3%) where (E)-phytol (53.4%) was the major compound followed by solanone (6.8%), cis-5-butyl-4-methyldihydrofuran-2(3H)-one (6.4%) and dihydro-β-ionone (5.2%). In addition, the antimicrobial activity of “Plovdiv 7” tobacco leaf aroma extracts (concrete and resinoid extract) are tested against nine microbial strains. The results show a weak antimicrobial activity against Staphylococcus aureus and Bacillus subtilis bacteria, whereas no activity is recorded for the other seven microbial strains tested.

کلیدواژه‌ها

Essential Oil؛ Extracts؛ Nicotiana tabacum L؛ Polyphenols؛ Triterpenes

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