Cloning and expression of rhl AB operon under the control of tac promoter in E. coli

International Journal of Molecular and Clinical Microbiology

مقاله 5، دوره 7، شماره 2، اسفند 2017، صفحه 855-868   

نوع مقاله: Research Article

نویسندگان

jamshid Raheb^{* 1}؛ seyed abolghassem mohammadi bondarkhilli¹؛ Mehdi MohMMADI²؛ Kambiz Akbari²؛ Hossein Shahbani³؛ Arefeh Alipour⁴

¹National Institute of Genetics and Biotechnology Engineering, Research Institute of Medical Biotechnology, Department of Molecular Medicine

²Natinal Institute of Genetic Engineering and Biotechnology

³National Institute of Genetic Engineering and Biotechnology

⁴National Institute of Genetic Rngineering and Biotechnology

چکیده

Today, efforts go towards the replacement of chemical surfactants by natural biological biosurfactants (biosurfactant), as these materials are not carcinogenic and highly compatibile with the environment. One of the main classes of biosurfactants is rhamnose containing glycolipid biosurfactant (rhamnolipids). This type of biosurfactants can be applied in many industries such as oil industry, pharmaceutical industry, food industry and esc. In the present study, with elimination of regulatory elements of monorhamnolipid operon, the suitable construct was made and transformed to E. coli BL12 and E. coli DH5α competent cells. Then the production of monorhamnolipid in recombinant E. coli BL12 harboring pET 12a vector and mono and di-rhamnolipid biosurfactant in recombinant E. coli DH5α harboring pTrc 99A evaluated by CTAB plate and thin layer chromatography (TLC). The relative production of mono and dirhamnolipid biosurfactant was determined by oil spreading method. Finally, the efficiency of the extracted biosurfactant was verified by E12 method in different salinity and pH conditions.

کلیدواژه‌ها

Biosurfactant؛ Rhamnolipid؛ E. coli BL12؛ E. coli DH5α؛ transformation