Evaluation of Mycoplasma Muris and Pulmonis infections in Genital and Respiratory Tracts

International Journal of Molecular and Clinical Microbiology

مقاله 1، دوره 8، شماره 1، شهریور 2018، صفحه 903-911   

نوع مقاله: Research Article

نویسندگان

Fatemeh Abedini^{* 1}؛ Mohammad Reza Zinatizadeh²؛ Abbas Ashtari³؛ mostafa jafarpour⁴؛ Mohammad Ebrahimi⁵؛ Golamreza shokri⁶؛ Roozbeh Fallahi⁷

¹Department of Laboratory Animal Breeding, Agricultural Research, Education and Extension Organization, Razi Vaccine and Serum Research Institute, PO Box 31975/148 Hessarak, Karaj, Alborz, Iran

²MSc in Genetics, Dept. of Biology, Tonekabon Branch, Islamic Azad University, Mazandaran, Iran

³Mycoplasma reference laboratory, Razi vaccine and serum research institute, Karaj, Iran

⁴Faculty of Biological Science, Islamic Azad University, Tonekabon Branch, Mazanderan, Tonekabon, Iran

⁵Quality Assurance, Razi Vaccine and Serum Research Institute, Karaj, Iran

⁶Research and Development Department, Agricultural Research, Education and Extension Organization, Razi Vaccine and Serum Research Institute,

⁷Department of Research & Development, Razi Vaccine and Serum Research Institute, Karaj, Iran

چکیده

Abstract
Mycoplasma pulmonis and muris infections have been associated with several diseases in conventionally housed laboratory rat and mice colonies. In naturally infected of mycoplasma pulmonis in mice and rat colonies, the respiratory organ appears to be the favored site of colonization; so, it was not surprising to see that infection spread from the site of urogenital tract to the respiratory tract. In this research, PPLO broth culture and polymerase chain reaction (PCR) assay was used to detect Mycoplasma pulmonis and muris contamination in mice strain NIH. A new species of Mycoplasma muris(MYMORazi) have been identified in the nasopharyngeal, lung and vaginal samples of mice strain NIH in Razi Vaccine and serum research institute. Despite using two pair's specific primers targeting 16SrRNA gene of Mycoplasma pulmonis, detection was failed in both organs. We propose that the Mycoplasma muris strain MYMO Razi can cause infection in both organs of mouse strain NIH without presence of Mycoplasma pulmonis.

کلیدواژه‌ها

Mycoplasma muris؛ Mycoplasma pulmonis؛ Laboratory Mice؛ Vaginal Infection؛ Lung Infection