Evaluation of Ice Nucleation Activity (INA) and INA Gene Detection in the Bacteria Isolated from Pistachio Trees in Kerman Province, Iran

Journal of Nuts

مقاله 5، دوره 09، شماره 2، اسفند 2018، صفحه 147-157   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22034/jon.2018.542997

نویسندگان

Mahdieh Rostami¹؛ Nader Hasanzadeh^{* 1}؛ Pezhman Khodaygan²؛ Ali Riahi Madvar³

¹Department of Plant Protection, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Plant protection Department, Faculty of Agriculture, Vali- E- Asr University of Rafsanjan, Iran

³Biotechnology Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran

چکیده

IIce nucleation active (INA) bacteria are common epiphytic inhabitants that cause frost damage in many plants in the near-zero temperatures. Yet, no studies were found in ice nucleation bacteria associated with pistachio trees. In our earlier study some INA strains were identified and reported. These were assigned as Pseudomonas fragi, P. putida, P. moraviensis and Pantoea agglomerans. In current work, two new strains namely P. viridiflava and Entrobacter cloacea were identified. Their ice nucleation frequency were evaluated and compared with above-mentioned ice positive strains isolated from pistachio trees. Pseudomonas fragi raf₃ was considered as the most ice nucleation active bacteria. This was followed by P. putida raf₆, P. moraviensis raf₁, P. moraviensis raf₅, Pantoea agglomeranse raf₇, P. viridiflava raf_2, Entrobacter cloacea raf₈ and Pseudomonas sp. raf₄, respectively. To detect INA genes, two sets of degenerate primers were used and partial INA gene sequences were amplified. INA gene sequence) 425bp) for Pseudomonas putida raf_6,  Pantoea agglomerans raf₇ and P. fragi raf₃ were amplified with primer pair of 3308/3463. Whereas, a fragment of 194bp was detected in Pseudomonas sp. raf₄,  P. moraviensis raf₅ and  P. moraviensis raf₁using forward and reverse primer pair of 3076/3463. Entrobacter cloacea raf₈ has reported for the first time as epiphytic ice plus strain. The capability of the latter as a bacterial biocontrol agent against insect pests was reported.

کلیدواژه‌ها

Ice nucleation bacteria؛ Ice nucleation frequency؛ Kerman province؛ Pistachio

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