Study of the antileukemic activity of Mimosa caesalpiniifolia Benth. ethanolic extract and fractions

Trends in Phytochemical Research

مقاله 2، دوره 2، شماره 3، آذر 2018، صفحه 127-134   

نوع مقاله: Original Article

نویسندگان

Gabriele Taumaturgo Mororó¹؛ José Roberto de Oliveira Ferreira²؛ Michel Mualén de Morais Alves¹؛ Nayana Bruna Nery Monção³؛ Laís Campos Teixeira de Carvalho-Gonçalves⁴؛ Antônia Maria das Graças Lopes Citó³؛ Paulo Michel Pinheiro Ferreira⁵؛ Fernando Aécio de Amorim Carvalho¹؛ Juan Carlos Ramos Gonçalves¹

¹Núcleo de Pesquisas em Plantas Medicinais, Universidade Federal do Piauí, Teresina-PI, Brasil

²Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina-PI, Brasil

³Departamento de Química, Universidade Federal do Piauí, Teresina-PI, Brasil

⁴Centro de Biotecnologia, Universidade Federal da Paraíba, João Pessoa-PB, Brasil

⁵Departamento de Biofísica e Fisiologia, Laboratório de Cancerologia Experimental, Universidade Federal do Piauí, Teresina-PI, Brasil

چکیده

Mimosa caesalpiniifolia Benth. is a native plant to northeastern Brazil, traditionally used in folk medicine, with several pharmacological activities reported including antibacterial, anti-inflammatory, and antitumor. The present study evaluated the antileukemic potential of M. caesalpiniifolia Benth. ethanolic extract (EtOH) and its n-hexanic (HexF) and dichloromethane (DCMF) fractions. Previous analysis by our team revealed the constituents of high relative abundance in EtOH, HexF, and DCMF, like phytol (11.7%), lupeol (14.7%), and betulinic acid (70.3%), respectively. In the MTT cell viability test, EtOH, HexF, and DCMF induced dose-dependent cytotoxicity in human chronic myeloid cells (K562), with IC50 of 153.6 ± 0.1, 118.40 ± 0.2, and 40.0 ± 0.1 μg/mL, respectively (p <0.05). Additionally, DCMF (6-800 μg/mL) presented minor toxicity against normal human erythrocytes and murine macrophage cells. DCMF induced similar antileukemic effects (IC50=64.2 ± 5.0 μg/mL) against human acute myeloid cells (HL-60). However, it did not exert antitumor activity on murine sarcoma (S180) cells (p >0.05).

کلیدواژه‌ها

Betulinic acid؛ Cytotoxicity؛ Leukemia؛ M. caesalpiniifolia Benth؛ Medicinal plants

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