Volatile oil composition, antioxidant and antimicrobial properties of Boerhavia erecta L. and Euphorbia hirta L.

Trends in Phytochemical Research

مقاله 7، دوره 2، شماره 3، آذر 2018، صفحه 171-178   

نوع مقاله: Original Article

نویسندگان

Omosalewa Olaoluwa^* ؛ Dorcas Moronkola؛ Olayombo Taiwo؛ Peremobowei Iganboh

Department of Chemistry, Faculty of Science, University of Ibadan, 200284, Ibadan, Nigeria

چکیده

This study investigated the volatile oil composition of Boerhavia erecta L. and Euphorbia hirta L. along with the corresponding antioxidant and antimicrobial properties. In thisregard, stems and roots of Boerhavia erecta together with aerial parts of Euphorbia hirta L. were used. Essential oils were obtained by hydrodistillation method using Clevenger-type apparatus, while identification and characterization were done by gas chromatography-mass spectrometry (GC-MS) technique. Antioxidant activities were evaluated by 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) method and the antimicrobial properties were assessed by agar diffusion method. GC-MS analyses revealed seven and twenty-four components in stem and root oils of Boerhavia erecta L., respectively representing 81.84% and 76.93% of the total oil contents with phytol (61.31%) and 9-octadecenamide (33.95%) as the most abundant components, respectively. Eleven compounds were identified in E. hirta L. oil representing 92.79% of the oil. The major compound of this essential oil was (Z)-9-octadecenamide (60.71%). The other minor components were methyl hexadecanoate (7.02%) and phytol (4.80%). B. erecta and E. hirta oils showed significant antioxidant activities compared to α-tocopherol. B. erecta L. stem oil inhibited the growth of Escherichia coli and Staphylococcus aureus bacterial strains, while root oil showed moderate inhibition against Salmonella typhi and Bacillus subtilis at inhibition zones of 14-20 mm over a concentration of 12.5-50%. The three oil samples displayed significant antifungal activities against Candida albicans. Chemical constituents of B. erecta L. and E. hirta L. oils may be responsible for these biological activities. These evaluations are reported for the first time.

کلیدواژه‌ها

Biological activities؛ Gas chromatography-mass spectrometry (GC-MS)؛ Phytol؛ Volatile oil

مراجع

Abdelmonim, O.A.H., Saad, M.H., 2015. Chemical composition and antimicrobial activity of Sudanese Lupinus termis L. root extracts. J. Pharm. Innov. 4(5), 1-4.

Aboaba, S.A., Choudhary, M.I., 2015. Chemical composition and biological activities of the volatile oils of Palisota hirsuta (Thunb) K. Schum and Trema orientalis (L) Blume. Int. J. Chem. 7(2), 21-26.

Adams, R.P., 2001. Identification of Essential Oil Composition by Gas Chromatography/ Quadrapole. Allured Publishing, Carol Stream, IL.

Adedapo, A.A., Abatan, M.O., Akinloye, A.K., Idowu, S.O., Olorunsogo, O.O., 2003. Morphaometric and histopathological studies on the effects of some chromatographic fractions of Phyllanthus amarus and Euphorbia hirta on the male reproductive organs of rats. J. Vet. Sci. 4(2), 181-185.

Adedapo, A.A., Shabi, O.O., Adedokun, O.A., 2005. Anthelminthic efficacy of the aqueous extract of Euphorbia hirta (Linn.) in Nigerian dogs. Arch. Vet. Sci. 75(1), 39-47.

Amutha, I.D.J., Kottai, M.A., 2014. Gas chromatography-mass spectrometry analysis of bioactive constituents in the ethanolic extract of Saccharum spontaneumlinn. Int. J. Pharm. Pharm. Sci. 6(2), 755-759.

Angeline, T., Sreenivasan, S., Lachimanan Y. L., Sivaramakrishnan S., Surash, R., (2010). Antioxidant activity and total phenolic content of methanol extracts of Ixora coccinea, Pharm. Biol. 48(10), 1119-1123.

Aworinde, D., Nwoye, D., Jayeola, A., Olagoke, A., Ogundele, A., 2009. Taxonomic significance of foliar epidermis in some members of Euphorbiaceae family in Nigeria. J. Bot.4(1), 17-28.

Calderón-Montaño J.M., Burgos-Morón E., Pérez-Guerrero, C., López-Lázaro, M., 2011. A review on the dietary flavonoid kaempferol. Mini-Rev. Med. Chem. 11(4), 298-344.

Camila, C.M.P.S., Mirian, S.S., Vanine, G.M., Luciana, M.C., Antonia, A.C.A., Guilherme, A.L.O., Jéssica, P.C., Damião, P.S., Rivelilson, M.F., Reinaldo, N.A., 2013. Antinociceptive and antioxidant activities of phytol in vivo and in vitro models. J. Neurosci. 2013, 1-9.

Camilo, C.J., Alves Nonato, C.d.F., Galvao-Rodrigues, F.F., Cosa, W.D., Clemente, G.G., Sobreira Macedo. M.A.C., da Costa, J.G.M., 2017. Acaricidal activity of essential oils: a review. Trends Phytochem. Res. 1(4), 183-198.

Costa, J.P., Islam, M.T., Santos, P.S., Ferreira, P.B., Oliveira, G.L., Alencar, M.V., Paz, M.F., Ferreira, É.L., Feitosa, C.M., Citó, A.M., Sousa, D.P., Melo-Cavalcante A.A. 2016. Evaluation of antioxidant activity of Phytol using non- and pre-clinical models. Curr. Pharm. Biotechnol.17(14), 1278-1284.

Ghaidaa, J.M., Mohammad, J.I.H., 2016. Anti-bacterial, antifungal activity and chemical analysis of Punica grantanum (Pomegranate peel) using GC-MS and FTIR spectroscopy. Int. J. Pharmacog. Phytochem. Res. 8(3), 480-494.

Haider, M.H., Imad, H.H., Ibraheem, O.A., 2016. Antimicrobial activity and spectral chemical analysis of methanolic leaves extract of Adiantum capillus-veneris using GC-MS and FT-IR spectroscopy. Int. J. Pharmacog. Phytochem. Res. 8(3), 369-385.

Jin, H.P., Yun, J.L., Yeon, H.K., Ki, S.Y., 2017. Antioxidant and antimicrobial activities of Quinoa (Chenopodium quinoa Willd.) seeds cultivated in Korea. Prev. Nutr. Food Sci. 22(3), 195-202.

Koli, M.C., Choudhary, R., Kumar, S., Thakur, S., Shukla, D., Saxena, R.C., 2002. An isoflavone glycoside from the stem of Euphorbia hirta Linn as antimalarial compound. Asian J. Chem. 14(3-4), 1673-1677.

Kumar, P.P., Kumaravel, S. Lalitha, C., 2010a. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr. J. Biochem. Res. 4(7), 191-195.

Kumar, S., Malhotra, R., Kumar, D., 2010b. Euphorbia hirta its chemistry, traditional and medicinal uses and pharmacological activities. Pharmacogn. Rev. 4(7), 58-61.

Kumar, S., Malhotra, R., Kumar, D., 2010c. Antidiabetic and free radicals scavenging potential of Euphorbia hirta flower extract. Indian J. Pharm. Sci. 72(4), 533-537.

Mohammedhosseini, M., Mahdavi, B., Akhlaghi, H., 2013. Characterization and chemical composition of the volatile oils from aerial parts of Eryngium bungei Bioss. (Apiaceae) by using traditional hydrodistillation, microwave assisted hydrodistillation and head space solid phase microextraction methods prior to GC and GC/MS analyses: a comparative approach. J. Essent. Oil Bear. Plants 16(5), 613-623.

Mohammedhosseini, M., Sarker, S.D., Akbarzadeh, A., 2017. Chemical composition of the essential oils and extracts of Achillea species and their biological activities. A review. J. Ethnopharmacol. 199, 257-315.

Moraes, J., de Oliveira, R.N., Costa, J.P., Junior, A.L.G., de Sousa, D.P., Freitas, R.M., 2014. Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasis mansoni. PLoS Negl. Trop. Dis. 8(1), e2617.

Nunes, H.S., Miguel, M.G., 2017. Rosa damascene essential oils: a brief review about chemical composition and biological properties. Trends Phytochem. Res. 1(3), 111-128.

Nugraha, A.S., 2009. Bioactive compounds from Boerhavia erecta Linnaeus: an African medicinal plant, Research thesis, School of Chemistry, University of Wollongong, retrieved online on 31 May, 2017.

Nugraha, A.S., Hilou, A., Vandegraaff, N., Rhodes, D.I., Haritakun, R., Keller, P.A., 2015. Bioactive glycosides from the African medicinal plant Boerhavia erecta L. Nat. Prod. Res. 29(20), 1954-1958.

Olaoluwa, O.O., Aiyelaagbe, O.O., 2015. Phytochemical investigation and antimicrobial screening of Cardiospermun grandiflorum (Sweet) [Sapindaceae]. Int. J. Pharma Sci. Res.6(2), 348-351.

Olaoluwa, O.O., Aiyelaagbe, O.O., Irwin, D., Reid, M., 2013. Novel anthraquinone derivatives from the aerial parts of Antigonon leptopus Hook & Arn. Tetrahedron 69(33), 6906-6910.

Olaoluwa, O.O., James, D.A., Adigun, O.A., 2018. Volatile oil analysis of aerial parts of Boerhavia coccinea (Mill.). Nat. Prod. Res. 32(8), 959-962.

Olaoluwa, O.O., Ogunbor, F., 2015. Phytochemical screening, antimicrobial properties and essential oil constituents of Combretum sordidum exell. Int. J. Pharm. Sci. Res. 6(3), 1176-1180.

Patil, K.S., Bhalsing R.S., 2016. Ethnomedicinal uses, phytochemistry and pharmacological properties of genus Boerhavia. J. Ethnopharmacol. 182, 200-220.

Pejin, B., Savic, A., Sokovic, M., Glamoclija, J., Ciric, A., Nikolic, M., Radotic, K., Mojovic, M., 2014. Further in vitro evaluation of antiradical and antimicrobial activities of phytol. Nat. Prod. Res. 28(6), 372-376.

Rao, K., Karthik, L., Humalai, E., Srinivasn, K., Kumar, G., 2010. Antibacterial and antifungal activity of Euphorbia hirta L. leaves - a comparative study. J. Pharm. Res.3(3), 548-549.

Shareef, M.I., Gupta Ayushi, S.M., Gupta, S., 2017. Antioxidant and anticancer study of Boerhavia erecta. Int. J. Curr. Microbiol. Appl. Sci. 6(9), 879-885.

Sharma, P., Mohan, L., Srivastava, C.N., 2009. Amaranthus oleracea and Euphorbia hirta: natural potential larvicidal agents against the urban Indian malaria vector, Anopheles stephensi Liston Culicidae. Parasitol. Res. 106(1), 171-176.

Stintzing, F.C., Kammerer, D., Schieber, A., Adama, H., Nacoulma, O.G., Carle, R., 2004. Betacyanins and phenolic compounds from Amaranthus spinosus L.and Boerhavia erecta L. Z. Naturforsch., 59c,1-8.

Suriyavathana, M., Paramesiwari, G., Penislus, S.S., 2012. Biochemical and antimicrobial study of Boerhavia erecta and Chromolaena odorata(L.) King & Robinson. Int. J. Pharm. Sci. Res. 3(2), 465-468.

Venkata, R.B., Samuel, L., Pardha, S.M., Narashimha, R.B., Naga, V.K.A., Sudhakar, M., Radhakrishnan, T., 2012. Antibacterial, antioxidant activity and GC-MS analysis of Eupatorium odoratum. Asian J. Pharm. Clin. Res. 5(2), 99-106.

Zead, H.,Khaldun, M.,Yuliya, T.,Refat, A.G.,Hassan, S., Hassan, A., 2014. Analysis of essential oil in Jerusalem artichoke (Helianthus tuberosus l.) leaves and tubers by gas chromatography-mass spectrometry. Adv. Pharm. Bull. 4(2), 521-526.