Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant

Journal of Chemical Health Risks

مقاله 6، دوره 2، شماره 2، شهریور 2012   

شناسه دیجیتال (DOI): 10.22034/jchr.2012.543994

نویسندگان

M. Momen Heravi^{* 1}؛ B. Haghi¹؛ A. Morsali¹؛ P. Ardalan²؛ T. Ardalan²

¹Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran

²Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran, young researcher club

چکیده

Reactions of free radicals and reactive oxygen species (ROS) with biological molecules in vivo play an important physiological role in many diseases. 2,2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. This study was undertaken to investigate the free radical-scavenging and antioxidant activities of Zinc, Vitamin C and mixture of them. UV-Vis spectrophotometry method was used to evaluate the ability of Zinc, Vitamin C and mixture of them to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (), in the presence of Zinc, Vitamin C and mixture of them were obtained (0.4209, 2.092 and 1.82 min-1) respectively, under pseudo-first-order conditions at 25 oC.

کلیدواژه‌ها

Antioxidant؛ DPPH؛ kinetic؛ Free radical؛ UV-Vis spectrometry

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