اثر اسانس رزماری بر جمعیت لاکتوباسیل‌ها و میزان هیستامین طی دوره رسیدن در پنیر سنتی لیقوان

بهداشت مواد غذایی

مقاله 4، دوره 8، 4 (32) زمستان، دی 1397، صفحه 35-47   

نوع مقاله: مقاله پژوهشی

نویسندگان

رامین شیشه گر¹؛ حمید میرزایی^{* 2}؛ خسرو محمدی³؛ گیتی کریم⁴؛ ودود رضویلر⁵

¹دانشجوی دکترای بهداشت مواد غذائی، دانشگاه آزاد اسلامی واحد تریز، تبریز، ایران

²دانشیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز، تبریز، ایران

³استادیار گروه بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد تبریز ، تبریز، ایران

⁴استادگروه بهداشت و کنترل کیفی مواد غذائی، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران

⁵استاد گروه بهداشت مواد غذایی، دانشکده علوم تخصصی دامپزشکی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تبریز، ایران

چکیده

مسمومیت هیستامینی یکی از نگرانی‌های مهم در سلامت پنیرهای سنتی رسیده  بوده و مهم‌ترین عامل تولید آن میکرارگانیسم‌های دکربوکسیله کننده در طی دوره رسیدن می‌باشد. هدف از مطالعه حاضرتعیین تأثیر اسانس رزماری بر جمعیت لاکتو باسیلوس‌ها و میزان هیستامین در پنیر لیقوان در طول دوره رسیدن با روش HPLC بود. ترکیب شیمیائی اسانس با استفاده از GC/MS مورد شناسائی قرار گرفت. نمونه‌های پنیر حاوی صفر، 125 و  ppm250 از اسانس رزماری تهیه شد. نمونه‌ها در روزهای صفر، 30، 90 و 150 دوره رسیدن از نظر تعداد باکترهای لاکتوباسیلوس و میزان هیستامین مورد آزمایش قرار گرفتند. آلفا‌پینن (Alfa pinene) با 1/11 درصد، کامفور ((Camphore با 2/10 درصد و لیمونن – د ( Limonene-d) با 9/5 درصد، 3 جزء عمده موجود در اسانس بود. میانگین تعداد لاکتوباسیل ها در روزهای 30، 90 و 150 دوره رسیدن در نمونه‌های حاوی 125 و ppm 250 اسانس رزماری به‌طور معنی‌داری کمتر از نمونه‌های شاهد بود (05/0>(P. در طول دوره رسیدن میزان هیستامین در نمونه‌‌های حاوی اسانس کمتر از نمونه‌های شاهد بوده و در انتهای دوره رسیدن میزان هیستامین در نمونه‌های شاهد، حاوی 125 و ppm 250 اسانس رزماری به‌ترتیب برابر 190، 170 و ppm  04/51 بود. در مجموع می‌توان گفت که غلظت‌های 125 و  ppm250 از اسانس رزماری می‌تواند به‌عنوان یک ماده نگه‌دارنده طبیعی در پنیرهای سنتی لیقوان استفاده شود.

کلیدواژه‌ها

اسانس رزماری؛ هیستامین؛ لاکتوباسیلوس‌ها؛ پنیر لیقوان؛ دوره رسیدن

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