Assessment Production of Natural Reactive Oxygen Species Affected on Dormancy Alleviation, Germination and Antioxidant System in Sunflower Seeds

Journal of Crop Nutrition Science

مقاله 5، دوره 4، شماره 3، آذر 2018، صفحه 56-67   

نویسندگان

Mahdi Shaaban^{* 1}؛ Esfandiar Hassani Moghaddam²؛ Ezatollah Nabati³؛ Zahra Rahmati Motlagh¹

¹Ph.D of Weed Science, University of Mohaghegh Ardabili, Ardabil, Iran.

²Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran.

³Assistant Professor, Department of Soil Science and Engineering University of Mohaghegh Ardabili, Ardabil, Iran.

چکیده

BACKGROUND: The active oxygen species, despite the damaging effects they have useful roles in the body are living things.
OBJECTIVES: This research was done to determine whether Reactive Oxygen Species (ROS) could mediate Cyanide and Methylviologen signal in seed dormancy alleviation and sunflower seed germination, more widely, to assess their putative role in the control of seed germination and antioxidant system.
METHODS: The experiment was laid out in completely randomized design with four replications. The treatment consisted non-dormant seed, dormant seeds, dormant seeds treated with Methylviologen (producing reactive oxygen species) and dormant seeds treated with hydrogen Cyanide (gas producing reactive oxygen species). For germination test 25 seed take in 9cm petridishes on filter paper. For biochemical assay seeds were imbibed for 24h and therefore Hydrogen peroxide (H₂O₂), Malondialdehyde (MDA) and four antioxidant enzymes such as Catalase (CAT), Proxidase (POX), Ascorbat proxidase (APX) and Superoxide dismutase (SOD) ware measured.
RESULT: Maximum seed germination was recorded at non-dormant seeds and minimum of it was measured at dormant seeds. Lowest H₂O₂ and MDA production and lowest APX activity was recorded at dormant seeds. However, highest three detoxified enzymes activities such as CAT, POX and SOD was founded at dormant seeds. This enzyme activity was coinciding with lowest ROS production such as H₂O₂ and MDA production. ROS production as resources such as Cyanide and Methylviologen play a key role in the control of sunflower dormancy alleviation and seed germination.
CONCLUSION: Main reason for seeds dormancy alleviation is production of ROS is acceptable level so germination of dormant seeds which was treated with Methylviologen and Cyanide was more than dormant control seeds and was similar to non-dormant seeds.

کلیدواژه‌ها

<i> Catalase؛ Cyanide؛ Malondialdehyde؛ Super oxide dismutase </i>

مراجع

Apel, K. and H. Hirt. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann. Rev. Plant Biol. 55: 373-399.

Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14: 93–107.

Bailly, C. A., F. Benamar, D. Corbineau. and D. Dome. 1996. Changes in malondialdehyde content and in superoxide dismutase, catalase and glutathione reductase activities in sunflower seed as related to deterioration during accelerated aging. Physiol. Plant. 97: 104-110.

Bailly, C., H. El-Maarouf-Bouteau. and F. Corbineau. 2008. From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. C. R. Biol. 331: 806–814.

Bazin, J., N. Langlade, P. Vincourt, S. Arribat, S. Balzergue, H. El-Maarouf- Bouteau. 2011. Targeted mRNA oxidation regulates sunflower seed dormancy alleviation during dry after-ripening. Plant Cell. 23: 2196–2208.

Bethke, P. C., I. G. L. Libourel, V. Reinohl. and R. L. Jones. 2006. Sodium nitroprusside, cyanide, nitrite, and nitrate break Arabidopsis seed dormancy in a nitric oxide-dependent manner. J. Planta. 223: 805–812.

Bethke, P. C., I. G. Libourel, N. Aoyama, Y. Y. Chung, D. W. Still. and R. L. Jones. 2007. The Arabidopsis aleurone layer responds to nitric oxide, gibberellin, and abscisic acid and is sufficient and necessary for seed dormancy. Plant Physiol. 143: 1173–1188.

Bewley, J. D. 1997. Seed germination and dormancy. Plant Cell. 9: 1055–1066.

Bewley, J. D. and M. Black. 1994. In seeds. Physiology of development and germination. 2^nd Ed. Plenum Press. New York. USA. 366p.

Blokhina, O., E. Virolainen. and K. V. Fagerstedt. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: A review. Ann. Bot. 91: 179–194.

Bogatek, R. and S. Lewak. 1988. Effect of Cyanide and cold treatment on sugar catabolism in apple seeds during dormancy removal. Physiol. Plant. 73: 406–411.

Calderbank, A. and P. Slade. 1976. Diquat and paraquat. In: P. C. Kearney, D. D. Kaufman. Eds. Herbicides. Chemistry Degradation and Mode of Action. 2^th Edition. Vol. 2. Marcel Dekker. New York. USA. pp: 501–533.

Chen, C. N. and S. M. Pan. 1996. Assay of superoxide dismutase activity by combining electrophoresis and densitometry. Bot. Bull. Acad. Sin. 37: 107–111.

Dalton, T. P., H. G. Shertzer. and A. Puga. 1999. Regulation of gene expression by reactive oxygen. Ann. Rev. Pharmacol Toxicol. 39: 67–101.

Elstner, E. F. and A. Heupel. 1976. Inhibition of nitrite formation from hydroxyl ammonium chloride: a simple assay for superoxide dismutase. Anal. Bio-chem. 70: 616–620.

Esashi, Y., Y. Ohhara, M. Okazaki. and K. Hishinuma. 1979. Control of cocklebur seed germination by nitrogenous compounds: nitrite, nitrate, hydroxylamine, thiourea, azide and Cyanide. Plant Cell Physiol. 20: 349–361.

Finch-Savage, W. E. and G. Leubner-Metzger. 2006. Seed dormancy and the control of germination. New Phytol. 171: 501–523.

Gay, C., F. Corbineau. and D. Come. 1991. Effects of temperature and oxygen on seed germination and seedling growth in sunflower (Helianthus annuus L.). Environ. Exp. Bot. 31: 193–200.

Giannopolitis, C. N. and S. K. Ries. 1977. Superoxide dismutases i. Occurrence in higher plants. Plant Physiol. 59: 309-314.

Gill, S. S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Bio-chem. 48: 909-930.

Goel, A. and L. S. Sheoran. 2003. Lipid peroxidation and peroxide scavenging enzymes in cotton seeds under natural ageing. Biol. Plant. 46: 429-434.

Grant, J. J. and G. J. Loake. 2000. Role of reactive oxygen intermediates and cognate redox signaling in disease resistance. Plant Physiol. 124: 21–30.

Heath, R. L. and L. Packer. 1968. Photoperoxidation in isolated choloroplast.1. Kinetics and stoichiometry of fatty acid peroxidation. Archive. Bio-chem. Biophysics. 12: 189-198.

Khajepoor, M. R. 2004. Industrial plants. Isfahan Technology Press. 564 pp.

Koornneef, M., C. Alonso-Blanco, L. Bentsink, H. Lankenstein-De Vries, I. Debeaujon, C. J. Hanhart, K. M. Leon-Kloosterziel, A. J. M. Peeters. and V. Raz. 2000. The Genetics of seed dormancy. CAB International. Wallingford. Oxon. UK. 465 pp.

Leubner-Metzger, G. 2005. Beta-1,3-glucanase gene expression in low-hydrated seeds as a mechanism for dormancy release during tobacco after-ripening. Plant J. 41: 133-45.

Leymarie, J., G. Vitkauskaite, H. H. Hoang, E. Gendreau, V. Chazoule, P. Meimoun, F. Corbineau, H. El-Maarouf-Bouteau. and C. Bailly. 2011. Role of reactive oxygen species in the regulation of Arabidopsis seed dormancy. Plant Cell Physiol. 53(1): 96–106.

Liszkay, A., E. Van Der Zalm. and P. Schopfer. 2004. Production of reactive oxygen intermediates (O₂.-, H₂O₂, and. OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 136: 3114-23.

Liu, Y., N. Ye, R. Liu, M. Chen. and J. Zhang. 2010. H₂O₂ mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy and germination. J. Exp. Bot. 61: 2979–2990.

Luck, H. 1962. Methods of enzymatic analysis. E.B. By Bergmeyer (1^th Edition). Verlag Chemie Weinheim. pp: 885-894.

McDonald, M. B. 1999. Seed deterioration: physiology, repair and assessment. Seed Sci. Tech. 27: 177–237.

Moller, I. M. 2001. Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annual Rev. Plant Physiol. Plant Mol. Biol. 52: 561–591.

Nakano, Y. and K. Asada. 1981. Hydrogen peroxide scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22: 867–880.

Oracz, K., H. El-Maarouf-Bouteau, R. Bogatek, F. Corbineau. and C. Bailly. 2008. Release of sunflower seed dormancy by Cyanide: cross-talk with ethylene signaling pathway. J. Exp. Bot. 59: 2241–2251.

Oracz, K., H. El-Maarouf-Bouteau, I. Kranner, R. Bogatek, F. Corbineau. and C. Bailly. 2009. The mechanisms involved in seed dormancy alleviation by hydrogen cyanide unravel the role of reactive oxygen species as key factors of cellular signaling during germination. Plant Physiol. 150: 495-505.

Oracz, K., H. El-Maarouf-Bouteau, M. Jill, F. K. Cooper, M. Belghazi, C. Job, D. Job, F. Corbineau. and C. Bailly. 2007. ROS production and protein oxidation as a novel mechanism for seed dormancy alleviation. The Plant J. 217: 1-14.

Philippe, M., R. K. Koraljka. and B. Karlheinz. 2007. A simple method to measure effective catalase activities: optimization, validation, and application in green coffee. Anal. Bio-Chem. 360: 207–215.

Roberts, E. H. 1973. Oxidative processes and the control of seed germination. In: W. Heydecker. Ed. Seed Ecol. Butterworth. London. UK. pp: 189–218.

Sakharov, I. Y. and G. B. Ardila. 1999. Variation of peroxidase activity in cacao beans during their ripening, fermentation and drying. Food Chem. 65: 51–54.

Schopfer, P., C. Plachy. and G. Frahry. 2001. Release of reactive oxygen intermediates (Super oxide radicals, hydrogen peroxide and hydroxyl radicals) and peroxidase in germinating radish seeds controlled by light, gibberellin, and abscisic acid. Plant Physiol. 125: 1591–1602.

Sung, J. M. 1996. Lipid peroxidation and peroxide-scavenging in soybean seeds during aging. Physiol. Plant. 97: 85–89.