تغییرات سیستم‌های پاداکساینده در انواع برگ‌های رنگی شمشاد طلایی (Euonymus japonicus Thunb.)

فیزیولوژی محیطی گیاهی

مقاله 4، دوره 12، شماره 45، فروردین 1396، صفحه 38-50   

نوع مقاله: پژوهشی

نویسندگان

نادر چاپارزاده^* ؛ سمانه صفی خانی؛ لیلا زرندی میاندوآب

گروه زیست‌شناسی، دانشگاه شهید مدنی آذربایجان، تبریز، ایران

چکیده

برخی نشانگرهای فیزیولوژیکی و پاسخ­های آنتی­اکسیدان در انواع برگ­های رنگی درختچه ‍زینتی همیشه سبز شمشاد طلایی (Euonymus japonicus Thunb.) مورد مطالعه قرار گرفت. این درختچه دارای سه نوع برگ­ به رنگ­های سبز تیره، سبز روشن و زرد می­باشد. در این سه نوع برگ نشانگرهای تنش اکسیداتیو (غلظت پراکسید ­هیدروژن، پایداری غشاهای سلولی و پراکسیداسیون چربی­ها)، فعالیت آنزیم پراکسیداز و برخی ترکیبات آنتی­اکسیدان (ترکیبات فنلی، پرولین و اسیدهای آمینه آزاد) مطالعه شدند. نوع برگ بر محتوای پراکسید هیدروژن، میزان پایداری غشاهای سلولی و پراکسیداسیون چربی­های غشایی تاثیر معنی­دار گذاشت. مقدار اسیدهای­آمینه آزاد و پرولین در برگ­های زرد به شکل معنی­داری از برگ­های سبز تیره و روشن بیشتر بود. بیشترین و کمترین مقدار ترکیبات فنولی آزاد به ترتیب در برگ­های سبز تیره و زرد وجود داشت. یک الگوی افزایش فعالیت آنزیم پراکسیداز در برگ­های سبز روشن نسبت به سبز­تیره و برگ­های زرد نسبت به برگ­های سبز روشن مشاهده شد. از داده­های حاضر می­توان نتیجه گرفت که با وجود فعالیت سیستم­های آنتی­اکسیدان، تغییر رنگ برگ­ها از سبز تیره تا زرد منجر به افزایش آسیب­های اکسیداتیو می­شود.

کلیدواژه‌ها

اکسیداتیو؛ ترکیبات فنلی؛ پراکسیداز؛ پراکسید هیدروژن؛ شمشاد

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