Effects of different medium on morphophysiological traits of Lippia citriodora H.B.K. during in vitro culture condition

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 12، دوره 5، 3 Special issue (in Persian or Farsi)، بهمن 2014، صفحه 143-150   

نوع مقاله: Original article

نویسندگان

Hassan Nourafcan^{* 1}؛ Fatemeh Sefidkon²؛ Amir Mousavi³؛ Mozaffar Sharifi⁴؛ Ahmad Khalighi¹

¹Department of Horticulture, College of Agriculture, Tehran Science and Research branch, Islamic Azad University, Tehran, Iran;

²Research Institute of Forests and Rangelands, Tehran, Iran;

³National Institute of Genetic Engineering and Biotechnology, Tehran, Iran;

⁴Department of Plant Sciences, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran;

چکیده

Background & Aim: Lemon verbena (Lippia citriodora H.B.K.) is a richly scented herb and has a long history as a sacred and medicinal plant. Select an appropriate medium in tissue culture is a necessity for success Experimental: To evaluate the effect of 10 medium of previous researches for Lippia micropropagation (1- MS medium supplemented with BAP (3 mgL^-1) + IBA (0.1 mgL^-1) + activated charcoal (1 gL^-1) 2- MS medium supplemented with BAP (1 mgL^-1) + IBA (0.5 mgL^-1) + activated charcoal (1 gL^-1) 3- 1/2MS 4- MS medium supplemented with BAP (5 mgL^-1) 5- MS medium supplemented with BAP (1 mgL^-1) 6- MS medium supplemented with activated charcoal (1 gL^-1) 7- MS medium supplemented with BAP (1 mgL^-1) + activated charcoal (1 gL^-1) 8- MS medium supplemented with BAP (1 mgL^-1) + AgNO₃ (5 mgL^-1) 9- MS medium supplemented with AgNO₃ (5 mgL^-1) 10- MS (control)), an experiment was conducted as a Completely Randomized Design (CRD) with three replicates and three explants per replication.  Results & Discussion: The results showed positive effects for BAP (5 mgL^-1) and somewhat active charcoal, but contrary to predictions, AgNO₃ had no effect on reducing senescence and leaf necrosis. Recommended applications/industries: Therefore, Based on the finding, the four medium (MS medium supplemented with BAP (5 mgL^-1)) with the highest rates of shoot fresh weight, leaf number, internodes number, shoot number, root fresh weight, callus diameter and root dry weight can be introduced as a suitable medium of tissue culture in this experimental conditions.

کلیدواژه‌ها

BAP؛ Charcoal؛ lemon verbena؛ medicinal plants؛ tissue culture

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