Comparison of Antiviral Effects of Chelidonium majus L. Hexane and Aqueous Extracts against Herpes Simplex Virus Type 1

International Journal of Molecular and Clinical Microbiology

مقاله 2، دوره 8، شماره 2، اسفند 2018، صفحه 993-1000   

نوع مقاله: Research Article

نویسندگان

shiva Mohammad¹؛ Gholamreza Amin²؛ Mohammad Hassan Pouriayevali³؛ Masoud Parsania^{* 4}

¹Department of Biotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

²Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

³Department of Arboviruses and Viral Hemorrhagic Fevers (National Reference Laboratory), Pasteur Institute of Iran, Tehran, Iran.

⁴Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran Iran.

چکیده

Herpes simplex virus type 1 (HSV-1) leads to various clinical manifestations in humans. Due to drug resistance, the use of herbs extracts for treatment has been considered as a significant solution. One of these compounds is Chelidonium majus L. herb extracts which is known as Mamiran in Iran. In this study, two aqueous and hexane extracts of Chelidonium majus L. were used to investigate the antiviral effects against HSV-1. The toxicity of hexane and aqueous extracts on HeLa cells was determined by MTT assay. Anti-viral effects of the extracts were evaluated directly, at different concentrations and times. The effect of the aforementioned extracts on reducing the virus replication was evaluated by quantitative Real-time PCR based on the glycoprotein D (gD) gene expression level of the herpes simplex virus. We observed that a 3500 μg/ml concentration of aqueous extract of C.majus and 600 μg/ml concentration of hexane extract were not toxic to HeLa cells. None of the extracts had a direct anti-viral effect on the virus. The aqueous extract at the above concentrations after 1, 2, 4, and 8 h of adsorption significantly reduced on virus replication. In contrast, the hexanic extract of this plant had no viral and inhibitory effect on HSV-1 replication. The aqueous extract of C.majus has an antiviral effect on HSV-1 compared to the hexane extract.

کلیدواژه‌ها

HSV-1؛ Chelidonium majus؛ Hexane Extract؛ Aqueous extract؛ Real-time PCR