Phytochemical analysis, phenolic content and antioxidant activity of methanolic extract of Caryopteris odorata D.Don.Robin

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 3، دوره 9، شماره 4، تیر 2019، صفحه 189-196   

نوع مقاله: Original article

نویسندگان

ARCHANA Joshi^{* 1}؛ Anil K. Pant²؛ Om Prakash¹؛ Marcin Stocki³؛ Valary A. Isidorov⁴

¹Department of Chemistry, College of Basic Sciences and Humanities , G.B. Pant University of Agriculture and Technology, Pantnagar, U.S. nagar-263145, Uttarakhand, India;

²Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India

³Faculty of Forestry , Białystok University of Technology, ul. Piłsudskiego 1A, 17-200 Hajnówka, Poland

⁴Faculty of Forestry, Białystok University of Technology, ul. Piłsudskiego 1A, 17-200 Hajnówka, Poland;

چکیده

Background & Aim:Caryopteris odorata is an aromatic shrub of family verbenaceae growing in tropical to subtropical regions. Various traditional applications of this plant have been documented guiding us to investigate the pharmacological activities and to further investigate the chemical composition.
Experimental: Methanolic extract of aerial parts of Caryopteris odorata was investigated for antioxidant activity and the major phyto-constituents and total phenolic content were screened. The antioxidant activity was determined by measuring the scavenging activity using DPPH radical scavenging, NO radical scavenging, so radical scavenging activity, Metal chelating and reducing power assay. The antimicrobial efficacy was determined using agar well diffusion method against gram positive as well as gram negative bacteria. Sensitivity in terms of zones of inhibition and phytochemical composition of the extracts were also determined. In vitro anti-inflammatory activity was evaluated using albumin denaturation method. Diclofenec Sodium was used as a standard drug for the study of anti-inflammatory activity.
Results: The results revealed that, methanolic extracts possess broad spectrum antibacterial activity against three microorganisms. Phytochemical analysis revealed the presence of diterpenes carbohydrates, glycosides, sterols and triterpenoids, aliphatic acids , tocopherols and phenolics. The methenolic extract exhibit strong antioxidant activity (IC₅₀ values for DPPH assay 164.60 ±0.37 mg/ml, for metal chelating 280.30 ± 3.28 mg/ml, for reducing assay 80.38 ± 0.79 mg/ml, respectively). The extract also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation with IC₅₀ value as 497.22 ± 1.34 mg/ml, respectively. From the result, it is concluded that the C.odorata possess a rich amount of different class of compounds and further the phytochemicals present in the C.odorata extract may be responsible for the antimicrobial, antioxidant and anti-inflammatory activity.
Recommended applications/industries: The results obtained from various activities suggest Caryopteris odorata application as natural alternative antioxidant, anti-inflammatory and antimicrobial reagent.

کلیدواژه‌ها

Caryopteris odorata؛ Antimicrobial؛ Antioxidant؛ phytochemicals

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