Influence of plant growth regulators (BA, TDZ, 2-iP and NAA) on micropropagation of Aglaonema widuri

Iranian Journal of Plant Physiology

مقاله 2، دوره 9، شماره 4، آبان 2019، صفحه 2901-2909   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2019.668856

نویسندگان

Behazd Kaviani^{* 1}؛ Shahram Sedaghathoor¹؛ Mohammad Reza Safari Motlagh²؛ Seddigeh Rouhi¹

¹Department of Horticultural Science, Islamic Azad University, Rasht Branch, Rasht, Iran

²Department of Plant Pathology, Islamic Azad University, Rasht Branch, Rasht, Iran

چکیده

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of A. widuri on the same medium for both shoots and roots induction. This condition decreases time duration and cost of micropropagation. Apical buds as explants were obtained from greenhouse-grown A. widuri and were established on Murashige and Skoog (MS) medium. Three cytokinins including N⁶-benzyladenine (BA; 0.00, 3.00, 3.50, and 4.00 mg l^-1), 1-phenyl-3-(1,2,3-thiaiazol-5-yl), urea (TDZ; 0.00, 0.50, and 1.00 mg l^-1), and N⁶-(2-isopentenyl) adenine (2-iP; 0.00 and 7.00 mg l^-1), along with one auxin [α-naphtalene acetic acid (NAA; 0.00, 0.10, 0.20, 0.30, and 0.40 mg l^-1) were studied for their effect on micropropagation of A. widuri. The plantlets were transferred to pots and grown in the greenhouse with a success rate of 95%. BA and NAA treatments as 3.00 mg l^-1+ 0.2 mg l^-1 recorded the highest shoot proliferation rate (number: 6.00 shoots and length: 7.75 cm per explant). Treatment of 4.00 mg l^-1 BA + 0.10 mg l^-1 NAA + 0.50 mg l^-1 TDZ produced maximum nodes (13.25 per explant). The largest number of leaves (4.25 per explant) was produced in the medium containing 3.50 mg l^-1 BA + 0.20 mg l^-1 NAA. Maximum root initiation and development (14.25 per explant) was obtained on the medium containing 3.00 mg l^-1 BA + 0.20 mg l^-1 NAA. The combination of 3.50 mg l^-1 BA + 0.20 mg l^-1 NAA was found to be the most suitable growth regulator for obtaining the highest root length (8.25 cm per explant).

کلیدواژه‌ها

Araceae؛ in vitro micropropagation؛ ornamental plants؛ phytohormones؛ tissue culture

مراجع

Ali, A., A. Munawar and S. Naz. 2007.'An in vitro study on micropropagation of Caladium bicolor'. International Journal of Agriculture and Biology,9: 731–735.

Atak, C. and O. Celik. 2009.'Micropropagation of Anthuriumandraeanum from leaf explants'. Pakistan Journal of Botany,41: 1155–1161.

Azza, M., K. El-Sheikh and M. K. Mutasim. 2010.'In vitro shoot micropropagation and plant establishment of an ornamental plant dumb cane (Dieffenbachia compacta)'. International Journal of Current Research,6:27–32.

Chen, J. and R. J. Henny. 2008.'Role of micropropagation in the development of ornamental foliage plant industry'. In: Teixeira daSilva, J.A. (ed.) Floriculture, Ornamental and Plant Biotechnology, vol. V. Global Science Books, London, pp. 206–218.

Chen, J., R. J. Henny, P. S. Davenant and C. T. Chao. 2006.'AFLP analysis of Nephthys (Syngonium podophyllum Schott) selected from somaclonal variants'. Plant Cell Reports, 24: 743–749.

Chen, J., D. B. McConnell, R. J. Henny and K. C. Everitt. 2003.'Cultural guidelines for commercial production of interior scape Aglaonema'. TFAS Extension EnH95, University of Florida, Florida, pp. 1–5.

Chen, W.L. 2006.'In Vitro Shoot Multiplication and Ex Vitro Physiology of Aglaonema'. Master Dissertation, National Taiwan University, p. 124.

Chen, W.L. and D. M. Yeh. 2007.'Elimination of in vitro contamination, shoot multiplication, and ex vitro rooting of Aglaonema'.Hort. Science,42:629–632.

Dewir, Y., D. Chakrabarty, E. Hahn and K. Paek. 2006.'A simple method for mass propagation of Spathiphyllum cannifolium using an airlift bioreactor'. In Vitro Cellular and Developmental-Biology, Plant,42: 291–297.

Fang, J.Y., Y. R. Hsul and F. C. Chen.2013.'Development of an efficient micropropagation procedure for Aglaonema ‘Lady Valentine’ through adventitious shoot induction and proliferation'. Plant Biotechnology, 30: 423–431.

Ghasempour, H., Zh. Soheilikhah, A. R. Zebarjadi, S. Ghasempour and N. Karimi. 2014. 'In vitro micro propagation, callus induction and shoot regeneration in safflower L. cv. Leaf'. Iranian Journal of Plant Physiology, 4 (2): 999-1004.

Henny, R. 2000.'Breeding ornamental aroids'. In: Callaway D.J. and M. B. Callaway (eds.). Breeding Ornamental Plants. Timber Press, Portland, pp. 121–132.

Henny, R.J. and J. Chen. 2003.'Cultivar development of ornamental foliage plants'. Plant Breeding Review,23: 245–290.

Hussein, M.M.M. 2004.'In vitro propagation of three species of Aglaonema plants'. Arab University of Journal of Agricultural Science, 12:405–423.

Laohavisuti, N. and M. Mitrnoi. 2005.'Micropropagation of Aglaonema simplex Proc'. Kasetsart University (Thailand) Annual Conference, 43:267–274.

Jahan, M., M. Islam, R. Khan, A. Mamun, G. Ahmed and L. Hakim. 2009.'In vitro clonal propagation of Anthurium (Anthurium andraeanum L.) using callus culture'. Plant Tissue Culture and Biotechnology, 19: 61–69.

Jain, S.M. and S. J. Ochatt. 2010.'Protocols for in vitro propagation of ornamental plants'. Springer Protocols, Humana Press.

Kaviani, B. 2014. 'Micropropagation of Mathhiola incana using BA and IBA'. Iranian Journal of Plant Physiology, 4 (3): 1071-1078.

Kaviani, B. 2015. 'Some useful information about micropropagation'. Journal of Ornamental Plants, 5 (1): 29-40.

Kozak, D. and M. Stelmaszczuk. 2009.'The effect of benzyladenine on shoot regeneration in vitro of Zantedeschia aethiopica ‘Green Goddess.’ Annals UMCS Horticulture, 19: 14–18.

Mariani, T.S., A. Fitriani, J. A. Teixeira da Silva, A. Wicaksono and T. F. Chia.2011.'Micropropagation of Aglaonema using axillary shoot explants'. International Journal of Basic Applied Science,11: 46–53.

Miri, S. M., A. Savari, K. Behzad and B. Mohajer Iravani. 2016. 'Promotion of callus initiation, shoot regeneration and proliferation in Lisianthus'. Iranian Journal of Plant Physiology, 6 (4): 1855-1860.

Murashige, T. and T. Skoog. 1962.'A revised medium for rapid growth and bioassays with tobacco tissue culture'. Physiology Plant,15:473–497.

Qu, L., J. Chen, R. J. Henny,Y. Huang, R. D. Caldwell and C. A. Robinson.2002.'Thidiazuron promotes adventitious shoot regeneration from pothos (Epipremnum aureum) leaf and petiole explants'. In Vitro Cellular and Developmental-Biology, Plant,38: 268–271.

Shen, X., J. Chen and M. Kane. 2007.'Indirect shoot organogenesis from leaves of Dieffenbachia cv. Camouflage'. Plant Cell, Tissue and OrganCulture,89: 83–90.

Siddique, I. and M. Anis. 2006.'Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L.'. Indian Journal of Biotechnology, 5: 303–308.

Yeh, D.M., W. Yang, F. Chang, M. Chung, W. Chen and H. Huang. 2007.'Breeding and micropropagation of Aglaonema'. Acta Horticulturae, 755:93–98.

Zhang, S., R. Jiang and H. Zhou.2004.'Study on rapid propagation of Aglaonema widuricv. ‘Golden Jewelry’'. Chinese Agricultural Science Bulletin, 20: 39–40.