Sodium nitroprusside and salicylic acid decrease antioxidant enzymes activity in soybean

Iranian Journal of Plant Physiology

مقاله 8، دوره 10، شماره 1، دی 2019، صفحه 3073-3077   

نوع مقاله: Short communications

شناسه دیجیتال (DOI): 10.30495/ijpp.2019.670792

نویسندگان

Leila Aalam؛ Mohammad Sedghi^* ؛ Omid Sofalian

Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Iran

چکیده

The present study investigated the effect of salicylic acid (SA) and sodium nitroprusside (SNP) on germination and activity of antioxidant enzymes in soybean under oxidative stress using a factorial experiment based on completely randomized design with three replications. Treatments included SA (0 and 1 mM), SNP (0, 30, and 60 µM), and H₂O₂ (0, 50, and 100 µM). Results showed that the highest germination percentage (97%) was related to 1 mM SA without SNP and H₂O₂ which was 6% greater than the control and the highest germination rate (0.72) was related to 1 mM SA and 60 µM SNP without H₂O₂ (6.63% greater than the control). The highest activity for superoxide dismutase (89.17 units mg ^-1 protein) was achieved by application of 100 µM H₂O₂ without SNP. In conclusion, oxidative stress increased all antioxidant enzymes while SNP application decreased the enzyme activity and stress severity.

کلیدواژه‌ها

soybean؛ germination؛ hydrogen peroxide؛ oxidative stress؛ catalase

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