Chemical composition and antioxidant activity of Acorus calamus L. accessions from different altitudes of Uttarakhand Himalayas

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 9، دوره 9، شماره 4، تیر 2019، صفحه 243-250   

نوع مقاله: Original article

نویسندگان

Archana Parki¹؛ Pinky Chaubey²؛ Om Prakash^{* 3}؛ Ravendra Kumar⁴؛ Anil K. Pant⁵

¹CHemistry, Ph. D. scholar G B PANT UNIVERSITY OF AGRICULTURE AND TECHNOLOGY

²Deptt. Chemistry, C.B.S.H. G. B. Pant University of Agriculture & Technology, Pantnagar-263145, Uttarakhand, India

³Deptt. Chemistry, CBSH, G.B. Pant University Agriculture & Technology, Pantnagar Uttarakhand

⁴Department of chemistry, C.B.S.&H., G.B. Pant University of Ag. & Tech. Pantnagar, U.S. NAGAR (263145) Uttarakhand, India

⁵Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar, U.S. Nagar, Uttarakhand, India

چکیده

Background & Aim:  Acorus calamus L. (Araceae) is an herb traditionally used in Indian and Chinees system of medicine. It is a perennial herb with long, cylindrical scented rhizomes which are creeping and extensively branched with the thickness up to 2.5 cm. The rhizomes are whitish internally and brownish externally in appearance. It is vernacularly known as Bach in India. The present investigation reveals the phytochemical screening and antioxidant activity of the rhizome and leaves methanolic extracts of A calamus from three different altitudes of Uttarakhand.
Experimental:  The extracts compositions of Acorus calamus rhizomes and leaves collected from three different altitudes of Uttarakhand Himalayas, India. The yield of extracts ranged from 0.3- 4.8% w/v, were analyzed by GC/MS and in-vitro antioxidant assay were done by different methods.
Results:  Over 65 contributing 80.90-90.55 % in different extracts among all the accessions were identified. The major compounds identified were β-asarone (44.9-51.9%), shyobunone (1.1%- 5.3%), Z-methyl isoeugenol (0.1%-2.4%), leinoleic acid (6.4%-18.9%), α-asarone(0.1%-4.6%) and Z-isoelemicin (2.2%-15.8%). The amount of phenolics in rhizome extracts, ranged from 4.10 mg-4.80 mg GAE/g respectively, whereas in leaf extracts the amounts were 2.40-3.26mg GAE/g respectively. All the extracts exhibited good in vitro antioxidant activity with the IC50 values ranging from 0.3- 4.8 % w/v determined by different methods compared to standard antioxidant.
Recommended applications/industries: Based on above observations it can be inferred that the herb may be a good source of bioactive compounds and can work as an antioxidant to prevent the oxidative deteriorative activity of food materials beside generation of database for its scientific and judicious in-situ exploitation.

کلیدواژه‌ها

Acorus calamus؛ Antioxidant activity؛ Total phenols؛ Asarone؛ Shyobunone

مراجع

Adams, R.P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass. Spectroscopy, 4th Edn. Allured Publishing Co., Carol Stream Illinois.

Adams, R.P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass. Spectroscopy, 4th Edn. Allured Publishing Co., Carol Stream Illinois.

Adfa, M., Livandri, F., Meita, N.P., Manaf, S., Ninomiya, M., Gustian, I., Putranto, A.M.H., Supriati, R. and Koketsu, M.  2015. Termiticidal activity of Acorus calamus Linn. rhizomes and its main constituents against Coptotermes curvignathus Holmgren.  Journal of Asia-Pacific Entomology, 18: 47-50.  

Adfa, M., Livandri, F., Meita, N.P., Manaf, S., Ninomiya, M., Gustian, I., Putranto, A.M.H., Supriati, R. and Koketsu, M.  2015. Termiticidal activity of Acorus calamus Linn. rhizomes and its main constituents against Coptotermes curvignathus Holmgren.  Journal of Asia-Pacific Entomology, 18: 47-50.  

Avadhani, M.N., Selvaraj, C.I., Rajasekharan, P.E., Rao, V.K., Munirajappa, H. and Tharachand, C. 2016. Genetic diversity analysis and chemical profiling of Indian Acorus calamus accessions from South and North-East India. Indian Journal of Biotechnology, 4: 560-567.

Avadhani, M.N., Selvaraj, C.I., Rajasekharan, P.E., Rao, V.K., Munirajappa, H. and Tharachand, C. 2016. Genetic diversity analysis and chemical profiling of Indian Acorus calamus accessions from South and North-East India. Indian Journal of Biotechnology, 4: 560-567.

Barua, C.C., Sen, S., Das, A.S., Talukdar, A., Hazarika, N.J., Barua, A.G., Barua, A.M. and Barua, I.A. 2014. Comparative study of the in vitro antioxidant property of different extracts of Acorus calamus Linn. Journal of Natural Product and Plant Resources, 4: 8-18

Barua, C.C., Sen, S., Das, A.S., Talukdar, A., Hazarika, N.J., Barua, A.G., Barua, A.M. and Barua, I.A. 2014. Comparative study of the in vitro antioxidant property of different extracts of Acorus calamus Linn. Journal of Natural Product and Plant Resources, 4: 8-18

Burits, M. and Bucar, F. 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14: 323-328.

Burits, M. and Bucar, F. 2000. Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research, 14: 323-328.

Chen, H.P., Yang, K., Zheng, L.S., You, C.X., Cai, Q. and Wang, C.F. 2015. Repellant and insecticidal activities of shyobunone and isoshyobunone derived from the essential oil of Acorus calamus rhizomes. Pharmacogonosy Magazine, 11: 675-681.

Chen, H.P., Yang, K., Zheng, L.S., You, C.X., Cai, Q. and Wang, C.F. 2015. Repellant and insecticidal activities of shyobunone and isoshyobunone derived from the essential oil of Acorus calamus rhizomes. Pharmacogonosy Magazine, 11: 675-681.

Darmstadt, G.L., Mao‐Qiang, M., Chi, E., Saha, S.K., Ziboh, V.A., Black, R.E., Santosham, M. and Elias, P.M. 2002. Impact of tropical oils on the skin barrier: possible implications for neonatal health in developing countries. Acta Paediatrica, 91: 546-554.

Darmstadt, G.L., Mao‐Qiang, M., Chi, E., Saha, S.K., Ziboh, V.A., Black, R.E., Santosham, M. and Elias, P.M. 2002. Impact of tropical oils on the skin barrier: possible implications for neonatal health in developing countries. Acta Paediatrica, 91: 546-554.

Devi, S.A. and Ganjewala, D. 2011. Antioxidant activities of methanolic extracts of sweet-flag (Acorus calamus) leaves and rhizomes. Journal of herbs, spices & medicinal plants, 17: 1-11.

Devi, S.A. and Ganjewala, D. 2011. Antioxidant activities of methanolic extracts of sweet-flag (Acorus calamus) leaves and rhizomes. Journal of herbs, spices & medicinal plants, 17: 1-11.

Diezel, W., Schulz, E., Shanks, M., and Heise, H. 1993. Plants oils: Topical application and anti-inflammatory effects (Croton oil test). Dermatologische Monatsschrift, 179: 173-173. 

Diezel, W., Schulz, E., Shanks, M., and Heise, H. 1993. Plants oils: Topical application and anti-inflammatory effects (Croton oil test). Dermatologische Monatsschrift, 179: 173-173. 

Dong, W., Yang, D. and Lu, R. 2010. Chemical constituents from the rhizome of Acorus calamus L. PlantMed, 76: 454-457.

Dong, W., Yang, D. and Lu, R. 2010. Chemical constituents from the rhizome of Acorus calamus L. PlantMed, 76: 454-457.

Hossain, M.S., Zaman, S., Haque, A.B.M.H., Bhuiyan, M.P.I., Khondkar, P. and Islam, M.R. 2008. Chemical and pesticidal studies on Acorus calamus rhizomes. Journal of Applied Sciences Research, 4: 1261-1266.

Hossain, M.S., Zaman, S., Haque, A.B.M.H., Bhuiyan, M.P.I., Khondkar, P. and Islam, M.R. 2008. Chemical and pesticidal studies on Acorus calamus rhizomes. Journal of Applied Sciences Research, 4: 1261-1266.

 Jeena, G.S., Punatha, H., Prakash, O., Chandra, M. and Kushwaha, K.P.S. 2016. Study on in vitro antioxidant potential of some cultivated Pleurotus species (Oyster mushroom). Indian Journal of  Natural Products and Resources, 5: 56-61.

 Jeena, G.S., Punatha, H., Prakash, O., Chandra, M. and Kushwaha, K.P.S. 2016. Study on in vitro antioxidant potential of some cultivated Pleurotus species (Oyster mushroom). Indian Journal of  Natural Products and Resources, 5: 56-61.

Joshi, N., Prakash, O. and Pant, A.K. 2012. Essential oil composition and in vitro antibacterial activity of rhizome essential oil and β-asarone from Acorus calamus L. collected from lower Himalayan Region of Uttarakhand. Journal of Essential Oil Bearing Plants, 15: 32-37.

Joshi, N., Prakash, O. and Pant, A.K. 2012. Essential oil composition and in vitro antibacterial activity of rhizome essential oil and β-asarone from Acorus calamus L. collected from lower Himalayan Region of Uttarakhand. Journal of Essential Oil Bearing Plants, 15: 32-37.

Kumar, R., Prakash, O., Pant, A.K., Hore, S.K., Chanotiya, C.S. and Mathela, C.S. 2009. Compositional variations and anthelmentic activity of essential oils from rhizomes of different wild populations of Acorus calamus L. and its major component, beta-asarone. Natural Product Communication, 4: 275-278.

Kumar, R., Prakash, O., Pant, A.K., Hore, S.K., Chanotiya, C.S. and Mathela, C.S. 2009. Compositional variations and anthelmentic activity of essential oils from rhizomes of different wild populations of Acorus calamus L. and its major component, beta-asarone. Natural Product Communication, 4: 275-278.

Kunwar, G., Prakash, O., Chandra, M. and Pant, A.K. 2013. Chemical composition, antifungal and antioxidant activities of Perilla frutescens (L.) syn. P. ocimoides L. collected from different regions of Indian Himalaya. Asian Journal of Traditional Medicine, 8:88-98.

Kunwar, G., Prakash, O., Chandra, M. and Pant, A.K. 2013. Chemical composition, antifungal and antioxidant activities of Perilla frutescens (L.) syn. P. ocimoides L. collected from different regions of Indian Himalaya. Asian Journal of Traditional Medicine, 8:88-98.

Letawe, C., Boone, M. and Pierard, G. E. 1998. Digital image analysis of the effect of topically applied linoleic acid on acne microcomedones. Clinical and Experimental Dermatology, 23: 56-58.

Letawe, C., Boone, M. and Pierard, G. E. 1998. Digital image analysis of the effect of topically applied linoleic acid on acne microcomedones. Clinical and Experimental Dermatology, 23: 56-58.

Lewis, H.W. and Elvin-Lewis, F.P.M. Medical Botany, Plants Affecting Human Health. 2003. Second Edition, 1.

Lewis, H.W. and Elvin-Lewis, F.P.M. Medical Botany, Plants Affecting Human Health. 2003. Second Edition, 1.

Matthaus, B. 2002. Antioxidant activity of extracts obtained from residues of different oilseeds.  Journal of Agricultural and Food Chemistry, 50: 3444-3452.

Matthaus, B. 2002. Antioxidant activity of extracts obtained from residues of different oilseeds.  Journal of Agricultural and Food Chemistry, 50: 3444-3452.

Mehrotra, S., Mishra, K.P., Maurya, R., Srimal, R.C., Yadav, V.S., Pandey, R. and Singh, V.K. 2003. Anticellular and immunosuppressive properties of ethanolic extract of Acorus calamus rhizome. International Immunopharmacology, 3: 53-61.

Mehrotra, S., Mishra, K.P., Maurya, R., Srimal, R.C., Yadav, V.S., Pandey, R. and Singh, V.K. 2003. Anticellular and immunosuppressive properties of ethanolic extract of Acorus calamus rhizome. International Immunopharmacology, 3: 53-61.

Nanda, B.L. 2014. Determination of phytochemicals and antioxidant activity of Acorus calamus rhizome. Journal of Drug Delivery and Therapeutics, 4: 117-121.

Nanda, B.L. 2014. Determination of phytochemicals and antioxidant activity of Acorus calamus rhizome. Journal of Drug Delivery and Therapeutics, 4: 117-121.

 Pandy, V., Jose, N., Subhash, H. 2009. CNS activity of methanol and acetone extracts of Acorus calamus leaves in mice. Journal of Pharmacology and Toxicology, 4:79-86.

 Pandy, V., Jose, N., Subhash, H. 2009. CNS activity of methanol and acetone extracts of Acorus calamus leaves in mice. Journal of Pharmacology and Toxicology, 4:79-86.

Phongpaichit, S., Pujenjob, N., Rukachaisirikul, V. and Ongsakul, M. 2005. Antimicrobial activities of the crude methanol extract of Acorus calamus Linn.  Songklanakarin Journal of Science and Technology, 27: 517-523.

Phongpaichit, S., Pujenjob, N., Rukachaisirikul, V. and Ongsakul, M. 2005. Antimicrobial activities of the crude methanol extract of Acorus calamus Linn.  Songklanakarin Journal of Science and Technology, 27: 517-523.

 Raina, V.K., Srivastava, S.K. and Syamasunder, K.V. 2003. Essential oil composition of Acorus calamus L. from the lower region of the Himalayas. Flavour and Fragrance Journal, 18: 18-20.

 Raina, V.K., Srivastava, S.K. and Syamasunder, K.V. 2003. Essential oil composition of Acorus calamus L. from the lower region of the Himalayas. Flavour and Fragrance Journal, 18: 18-20.

Sethi, S., Prakash, O., Punetha, H. and Pant, A.K. 2016. Antioxidant assay and antifungal activity of essential oil and various extracts from Alpinia allughas Roscoe: A potent Zingiberaceous Herb. Journal of Essential Oil Bearing Plants, 19: 358-367.

Sethi, S., Prakash, O., Punetha, H. and Pant, A.K. 2016. Antioxidant assay and antifungal activity of essential oil and various extracts from Alpinia allughas Roscoe: A potent Zingiberaceous Herb. Journal of Essential Oil Bearing Plants, 19: 358-367.

Shahidi, F., Janitha, P.K. and Wanasusdara, P.D. 1992. Phenolic antioxidants. Critical Reviews in Food Science & Nutrition; 32: 67–103.

Shahidi, F., Janitha, P.K. and Wanasusdara, P.D. 1992. Phenolic antioxidants. Critical Reviews in Food Science & Nutrition; 32: 67–103.

Shetty, K., Curtis, O.F., Levin, R.E., Witkowsky, R. and Ang, W. 1995. Prevention of vitrification associated with in vitro shoot culture of Oregano (Origanum vulgare) by Pseudomonas spp.  Journal of Plant Physiology, 147: 447-451.

Shetty, K., Curtis, O.F., Levin, R.E., Witkowsky, R. and Ang, W. 1995. Prevention of vitrification associated with in vitro shoot culture of Oregano (Origanum vulgare) by Pseudomonas spp.  Journal of Plant Physiology, 147: 447-451.

Siddhuraju, P., Mohan,  P.S. and Becker, K. 2002. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chemistry, 79: 61-67.

Siddhuraju, P., Mohan,  P.S. and Becker, K. 2002. Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): a preliminary assessment of crude extracts from stem bark, leaves, flowers and fruit pulp. Food Chemistry, 79: 61-67.

Skerget, M., Kotnik, P., Hadolin, M., Hras, A.R., Simonic, M. and Knez, Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89: 191-198.

Skerget, M., Kotnik, P., Hadolin, M., Hras, A.R., Simonic, M. and Knez, Z. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chemistry, 89: 191-198.