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Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844) | ||
International Aquatic Research | ||
مقاله 5، دوره 9، شماره 1، فروردین 2017، صفحه 53-59 اصل مقاله (747.87 K) | ||
نوع مقاله: Original research | ||
شناسه دیجیتال (DOI): 10.1007/s40071-017-0155-4 | ||
نویسندگان | ||
Seinen Chow* 1؛ Nobuaki Suzuki2؛ Yoji Nakamura1؛ Motoshige Yasuike1؛ Kenji Saitoh1؛ Takashi Yanagimoto1 | ||
1National Research Institute of Fisheries Science, 2-12-4 Fukuura, Kanagawa, 236-8648, Japan | ||
2National Research Institute of Far Seas Fisheries, 5-7-1 Orido, Shizuoka, 424-8633, Japan | ||
چکیده | ||
Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared. Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeting introns were designed. These primer pairs amplified expected size of fragments in five congeneric tuna species, and utility of these primer pairs was investigated using distant fish species. | ||
کلیدواژهها | ||
Thunnus orientalis, Four calmodulin genes, Locus-specific primers, Cross-species؛ amplification, DNA marker | ||
آمار تعداد مشاهده مقاله: 133 تعداد دریافت فایل اصل مقاله: 79 |