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Wang, Jingjing, Zhao, Wei, Liang, Yajie, Han, Yifan, Wang, Xingbiao, Zhang, Xiaoxia, Ma, Xiaomei, Zhang, Demin, Huang, Zhiyong. (1395). Development of a prototype 16S rRNA gene-based microarray for monitoring planktonic actinobacteria in shrimp ponds. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 9(1), 71-80. doi: 10.1007/s40071-017-0158-1
Jingjing Wang; Wei Zhao; Yajie Liang; Yifan Han; Xingbiao Wang; Xiaoxia Zhang; Xiaomei Ma; Demin Zhang; Zhiyong Huang. "Development of a prototype 16S rRNA gene-based microarray for monitoring planktonic actinobacteria in shrimp ponds". نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 9, 1, 1395, 71-80. doi: 10.1007/s40071-017-0158-1
Wang, Jingjing, Zhao, Wei, Liang, Yajie, Han, Yifan, Wang, Xingbiao, Zhang, Xiaoxia, Ma, Xiaomei, Zhang, Demin, Huang, Zhiyong. (1395). 'Development of a prototype 16S rRNA gene-based microarray for monitoring planktonic actinobacteria in shrimp ponds', نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 9(1), pp. 71-80. doi: 10.1007/s40071-017-0158-1
Wang, Jingjing, Zhao, Wei, Liang, Yajie, Han, Yifan, Wang, Xingbiao, Zhang, Xiaoxia, Ma, Xiaomei, Zhang, Demin, Huang, Zhiyong. Development of a prototype 16S rRNA gene-based microarray for monitoring planktonic actinobacteria in shrimp ponds. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 1395; 9(1): 71-80. doi: 10.1007/s40071-017-0158-1

Development of a prototype 16S rRNA gene-based microarray for monitoring planktonic actinobacteria in shrimp ponds

International Aquatic Research
مقاله 7، دوره 9، شماره 1، فروردین 2017، صفحه 71-80    اصل مقاله (584.37 K)
نوع مقاله: Original research
شناسه دیجیتال (DOI): 10.1007/s40071-017-0158-1
نویسندگان
Jingjing Wang1؛ Wei Zhao1؛ Yajie Liang1؛ Yifan Han1؛ Xingbiao Wang1؛ Xiaoxia Zhang1؛ Xiaomei Ma1؛ Demin Zhang2؛ Zhiyong Huang* 1
1Tianjin Key Laboratory of Industrial Biological Systems and Process Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, No. 32, West 7th Road, Tianjin Airport Economic Area, Tianjin, 300308, China
2School of Marine Sciences, Ningbo University, Ningbo, 315211, China
چکیده
Many reports have shown that the composition of the bacterioplankton community can serve as a biological indicator to evaluate the occurrence of shrimp diseases. However, the distribution, diversity, and function of planktonic actinobacteria in shrimp ponds are still poorly understood. In this study, a prototype of a 16S rRNA gene-based taxonomic microarray was developed and evaluated for monitoring of planktonic actinobacteria in shrimp ponds. The prototype microarray is composed of 30 probes that target ten dominant families of planktonic actinobacteria found in shrimp ponds. The specificity of the actinobacterial microarray was validated by a set of control hybridizations with 16S rRNA genes clones. The prototype microarray was subsequently tested with two seawater samples from ponds with diseased shrimp populations (PDS) and ponds with healthy shrimp populations (PHS). The actinobacteria hybridization profiles revealed a lower abundance of Microbacteriaceae and a higher abundance of Mycobacteriaceae in PDS than in PHS. The changes in planktonic actinobacterial communities were validated by pyrosequencing data. These results support the utility of the microarray for monitoring planktonic actinobacteria in shrimp ponds and aquaculture environments.
کلیدواژه‌ها
Microarray؛ Planktonic actinobacteria؛ Shrimp ponds؛ 16S؛ rRNA gene؛ Aquaculture environment
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تعداد مشاهده مقاله: 151
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