Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus

International Aquatic Research

مقاله 6، دوره 7، شماره 2، شهریور 2015، صفحه 143-150   

شناسه دیجیتال (DOI): 10.1007/s40071-015-0101-2

نویسندگان

Hideyuki Nakagawa^{* 1}؛ Kuniko Nagasaka²؛ Hitomi Sakai³؛ Kozue Edo⁴؛ Mitsuko Shinohara⁵؛ Kiyoshi Ohura⁵

¹Division of Environmental Symbiosis, Graduate School of Integrated Arts and Sciences, Tokushima University, Tokushima 770-8502, Japan

²Fukae Nagasaka Clinic, Etajima 737-2214, Japan

³Division of Environmental Symbiosis, Graduate School of Integrated Arts and Sciences, Tokushima University

⁴Junior College Division, Department of Science for Human Health, Shikoku University, Tokushima 771-1192, Japan

⁵Department of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan

چکیده

A novel lectin was purified from the dorsal spines of the devil stinger, Inimicus japonicus using a combination of affinity chromatography techniques. A single band was detected on a native PAGE gel with a relative molecular mass of 97 kDa. The N-terminal partial amino acid of the intact 75 kDa subunit of the 97 kDa lectin was found to be DHEDS. The agglutination of rabbit erythrocytes by the 97 kDa lectin was inhibited most effectively by methyl α-D-mannoside. The 97 kDa lectin stimulated mitogenesis in murine splenocytes. This is the first study to examine the dorsal lectin of I. japonicus and one of the very few studies on venom lectins from venomous scorpaeniform fish. These results suggest that the devil stinger, I. japonicus, may be a novel resource for biologically active substances.

کلیدواژه‌ها

Devil stinger؛ Inimicus japonicus؛ Dorsal spine؛ Lectin؛ Agglutination؛ Mitogenic activity؛ Murine splenocytes