In vitro propagation of orchid Phalaenopsis amabilis (L.) Blume var. Jawa

Iranian Journal of Plant Physiology

مقاله 3، دوره 10، شماره 2، فروردین 2020، صفحه 3113-3123   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2020.672571

نویسندگان

Mozhdeh Asa؛ Behzad Kaviani^*

Department of Horticultural Science, Islamic Azad University, Rasht Branch, Rasht, Iran

چکیده

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in combination. A combination of 1.00 mg l^-1 KIN + 1.00 mg l^-1 IBA was found to be suitable for regeneration of most measured characteristics especially maximum PLBs regeneration (30.40/plantlet), leaf number (5.93/plantlet), and root number (8.36/plantlet) from protocorm explants. The maximum number of plantlets (11.66) was calculated on MS medium supplemented with 1.00 mg l^-1 KIN + 0.50 mg l^-1 IBA, followed by 1.00 mg l^-1 KIN + 1.00 mg l^-1 IBA (10.33). Plantlets were transplanted to pots filled with cocochips and sphagnum moss (70:30) for acclimatization and transferred to the greenhouse. Upon ex vitro transfer, 90% of plants survived.

کلیدواژه‌ها

micropropagation؛ ornamental plants؛ plant growth regulators؛ protocorm like bodies؛ tissue culture

مراجع

Arditti, J. and R. Ernst, R. 1993. 'Micropropagation of orchids'. John Wiley and Sons, New York.

Arditti, J., R. Ernest, T. W. Yam and C. Glabe. 1990. 'The contribution to orchid mycorhizal fungi to seed germination: a speculative review'. Lindleyana, 5: 249-255.

Balilashaki, Kh. and M. Ghasemi Ghehsareh. 2016. 'Micropropagation of Phalaenopsis amabilis var. ‘Manila’ by leaves obtained from in vitro culturing the nodes of flower stalks'. Notulae Scientia Biologicae, 8 (2): 164-169.

Balilashaki, Kh., R. Naderi, S. Kalantari and A. Soorni. 2014. 'Micropropagation of Phalaenopsis amabilis cv. Cool 'Breeze' with using of flower stalk nodes and leaves of sterile obtained from node cultures'. International Journal of Farming and Allied Sciences, 3 (7): 823-829.

Baker, B., B. Kaviani, Gh. Nematzadeh and N. Negahdar. 2014. 'Micropropagation of Orchis catasetum – A rare and endangered orchid'. Acta Scientiarum Polonorum-Hortorum Cultus, 13 (2): 197-205.

Bektas, E., M. Cuce and Sokmen, A. 2013. 'In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid'. Turkish Journal of Botany, 37: 336-342.

Chen, J. and W. C. Chang. 2006. 'Direct somatic embryogenensis and plant regeneration from leaf explants Phalaenopsis amabilis'. Biologia plantarum, 50 (2): 169-173.

Chugh, S., S. Guha and U. Rao. 2009. 'Micropropagation of orchids: A review on the potential of different explants'. Scientia Horticulturae, 122: 507-520.

Decruse, W., A. Gangaprasad, S. Seeni and S. V. Menon. 2003. 'A protocol for shoot multiplication from foliar meristem of Vanda spathulata (L.) Spreng'. Indian Journal of Experimental Biology, 41: 924-927.

Dev, C. R. and Temjensangba, 2006. 'In vitro propagation of threatened terrestrial orchid Malaxis khasiana Soland ex. Swartz through immature seed culture'. Indian Journal of Experimental Biology, 44: 762-766.

Giridhar, P., B. Obul Reddy and G. A. Ravishankar, 2001. 'Silver nitrate influences in vitro shoot multiplication and root formation in Vanilla planifolia Androl'. Current Science, 81: 1166–1170.

Hossain, M. M., M. Sharma, J. A. Teixeira da Silva and P. Phthak, 2010. 'Seed germination and tissue culture of Cymbidium giganteum Wall. Ex. Lindl'. Scientia Horticulturae, 123: 479-487.

Huan, L. V. T., T. Takamura and M. Tanaka, 2004. 'Callus formation and plant regeneration from callus through somatic embryo structures in Cymbidium orchid'. Plant Science, 166: 1443–1449.

Janarthanam, B. and S. Seshadri, 2008. 'Plantlet regeneration from leaf derived callus of Vanilla planifolia Androl'. In vitro Cellular and Developmental Biology - Plant, 44: 84-89.

Kalimuthu, K., R. Senthikumar and N. Murugalatha. 2006. 'Regeneration and mass multiplication of Vanilla planifolia Androl - a tropical orchid'. Current Science, 91: 1401-1403.

Kalimuthu, K., R. Senthikumar and S. Vijayakumar, 2007. 'In vitro micropropagation of orchid, Oncidium sp. (Dancing Dolls)'. African Journal of Biotechnology, 6 (10): 1171-1174.

Kim, M. S. and J. Y. Kim, 2003. 'Micropropagation of Dendrobium hybrids through shoot tip culture'. Acta Horticulturae, 624: 527-533.

Le, B. V., N. T. Hang Phuong, L. T. Anh Hong and K. T. Thanh Van, 1999. 'High frequency shoot regeneration from Rhynchostylis gigantea (Orchidaceae) using thin cell layers'. Plant Growth Regulation, 28: 179–185.

Luo, J. P., Y. Wang, X. Q. Zha and L. Huang, 2008. 'Micropropagation of Dendrobium densiflorum Lindle. ex Wall. through protocorm-like bodies: effect of plant growth regulators and lanthanoids'. Plant Cell, Tissue and Organ Culture, 93: 333-340.

Luo, J. P., C. Wawrosch and B. Kopp, 2009. 'Enhanced micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng through protocorm-like bodies: The effect of cytokinins, carbohydrate sources and cold pretreatment'. Scientia Horticulturae, 123: 258-262.

Luo, J. P., X. Q. Zha and S. T. Jiang, 2003a. 'Suspension culture of protocorm-like bodies from the endangered medicinal plant Dendrobium huoshanenese'. China Journal of Chinese Materials Medicine, 28: 611–614.

Mahendran, G. and V. Narmatha Bai. 2009. 'Mass propagation of Satyrium nepalense D. Don.—A medicinal orchid via seed culture'. Scientia Horticulturae, 119: 203–207.

Malabadi, R. B., G. S. Mulgund and N. Kallappa. 2005. 'Micropropagation of Dendrobium nobile from shoot tip sections'. Journal of Plant Physiology, 162: 473-478.

Malabadi, R. B., J. A. Teixeira da Silva, K. Nataraja and G. S. Mulgund. 2008. 'Shoot tip transverse thin cell layers and 2,4-epibrassinolide in the micropropagation of Cymbidium bicolor Lindl'. Floriculture and Ornamental Biotechnology, 2: 44–48.

Martin, K. P. and J. Madassery. 2006. 'Rapid in vitro propagation of Dendrobium hybrids through direct shoot formation from foliar explants, and protocorm-like bodies'. Scientia Horticulturae, 108: 95–99.

Murashige, T. and F. Skoog. 1962. 'A revised medium for rapid growth and bioassays with tobacco tissue cultures'. Physiology Plant. 15: 473–479.

Nagaraju, V., S. P. Das, P. C. Bhutia and R. C. Upadhyaya. 2003. 'Response of Cymbidium lunavian Atlas protocorms to media and benzyl aminopurine'. Indian Journal of Horticulture, 60 (1): 98–103.

Nagaraju, V. and S. K. Mani. 2005. Rapid in vitro propagation of orchid Zygopetalum intermedium.Journal of Plant Biochemistry and Biotechnology, 14: 27–32.

Nasiruddin, K. M., R. Begum and S. Yasmin. 2003. 'Protocorm-like bodies and plantlet regeneration from Dendrobium formosum leaf callus'. Asian Journal of Plant Science, 2: 955-957.

Nayak, N. R., S. Sahoo, S. Patnaik and S. P. Rath. 2002.  'Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium alofolium (L.) SW. and Dendrobium nobile Lindl. (Orchidaceae)'. Scientia Horticulturae, 94: 107-116.

Nge, K. L., N. New, S. Chandrkrachang and W. F. Stevens. 2006. 'Chitosan as a growth stimulator in orchid tissue culture'. Plant Science, 170: 1185-1190.

Nongdam, P., C. Nirmala and R. Tewari. 2006. 'In vitro multiplication of Cymbidium pendulum orchids via embryo culture'. Plant Cell Biotechnology and Molecular Biology, 7: 145–150.

Novotna, K. W., H. Vejsadova and P. Kindlemann. 2007. 'Effect of sugars and growth regulators on in vitro growth of Dactylorhiza species. Biologia Plantarum, 51: 198-200.

Panwar, D., K. Ram and H. N. S. Shekhawat. 2012. 'In vitro propagation of Eulophia nuda Lindl., an endangered orchid'. Scientia Horticulturae, 139: 46–52.

Park, S. Y., E. C. Yeung, D. Chakrabarty and K. Y. Paek. 2002. 'An efficient direct induction of protocorm- like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture'. Plant Cell Report, 21: 46–51.

Parthibhan, S., M. V. Rao and T. S. Kumar. 2015. 'In vitro regeneration from protocorms in Dendrobium aqueum Lindley – An imperiled orchid'. Journal of Genetic Engineering and Biotechnology, 13: 227-233.

Puchooa, D. 2004. 'Comparison of different culture media for the in vitro culture of Dendrobium (Orchidaceae)'. Indian Journal of Agriculture and Biology, 6: 884-888.

Ram, K., N. S. Shekhawat. 2011. 'Micropropagation of commercially cultivated Henna (Lawsonia inermis) using nodal explants'. Physiology and Molecular Biology of Plants, 17: 281–289.

Roy, A. R., R. S. Patel, S. Sajeev and C. Deka. 2011. 'Asymbiotic seed germination, mass propagation and seedling development of Vanda coerulea Griff ex. Lindle. (Blue Vanda): An in vitro protocol for an endangered orchid'. Scientia Horticulturae, 128: 325-331.

Roy, J. and N. Banerjee. 2003.' Induction of callus and plant regeneration from shoot tip explants of Dendrobium fimbriatum Lindl. var. Oculatum H.K.f.' Scientia Horticulturae, 97: 333-340.

Saiprasad, G. V. S., P. Raghuveer, S. Khetarpal and R. Chandra. 2004. 'Effect of various polyamines on production of protocorm-like bodies in orchid- Dendrobium 'Sonia'. Scientia Horticulturae, 100: 161-168.

Seeni, S. and P. G. Latha. 2000. 'In vitro multiplication and ecorehabilitation of the endangered Blue Vanda'. Plant Cell, Tissue and Organ Culture, 61: 1-8.

Sheelavantmath, S. S., H. N. Murthy, A. N. Pyati, H. G. Ashok Kumar and B. V. Ravishanker. 2000. 'In vitro propagation of the endangered orchid, Geodorum densiflorum (Lam.) Schltr. through rhizome section culture'. Plant Cell, Tissue and Organ Culture, 60: 151-154.

Shimura, H. and Y. Koda. 2004. 'Micropropagation of Cypripedium macranthos var. Rebunerse through protocorm-like bodies derived from mature seed'. Plant Cell, Tissue and Organ Culture, 78: 273-276.

Sinha, P., M. K. Hakim and M. F. Alam. 2007. 'Efficient micropropagation of Phalaenopsis amabilis (L.) B.L. cv. 'Cool Breeze' using inflorescence axis thin section as explants'. Propagation of Ornamental Plants, 7: 9-15.

Subramanium, G. and R. M. Taha. 2003. 'Morphogenesis of Cymbidium atropurpureum in vitro'. Malaysian Journal of Science, 22: 1-5.

Teixeira da Silva, J. A. 2003. 'Thin cell layer technology in ornamental plant micropropagation and biotechnology'. African Journal of Biotechnology, 2: 683-691.

Teixeira da Silva, J. A., T. Yam, S. Fukai, N. Nayak and M. Tanaka, M. 2005. 'Establishment of optimum nutrient media for in vitro propagation of Cymbidium Sw (Orchidaceae) using protocorm-like body segments'. Propagation of Ornamental Plants, 5: 129-136.

Teixeira da Silva, J. A., N. Singh and M. Tanaka. 2006. 'Priming biotic factors for optimal protocorm-like body and callus induction in hybrid Cymbidium (Orchidaceae), and assessment of cytogenetic stability in regenerated plants'. Plant Cell, Tissue and Organ Culture, 84: 135-144.

Teixeira da Silva, J. A., D. T. T. Giang, M. T. Chan, A. Sanjaya, A. Norikane, M. L. Chai, J. Chico-Ruı´z, S. Penna, T. Granstro¨m and M. Tanaka. 2007a. 'The influence of different carbon sources, photohetero-, photoauto- and photomixotrophic conditions on protocorm-like body organogenesis and callus formation in thin cell layer culture of hybrid Cymbidium (Orchidaceae)'. Orchid Science and Biotechnology, 1: 15–23.

Teixeira da Silva, J. A., A. Norikane and M. Tanaka. 2007b. 'Cymbidium: successful in vitro growth and subsequent acclimatization'. Acta Horticulturae, 748: 207–214.

Teng, W. L., L. Nicholson and M. C. Teng. 1997. 'Micropropagation of Spathoglottis plicata'. Plant Cell Report, 16: 831–835.

 

Wang, Y. F., R. J. Lu, Y. F. Sun, R. M. Zhou and J. H. Huang. 2004. 'The induction and cultivation of cell-derived regenerates of Dendrobium huoshanenese'. Acta Agriculturae Shanghai, 20: 8-10.

Wu, I. F., J. T. Chen and W. C. Chang. 2004. 'Effect of auxins and cytokinins on embryo formation from root-derived callus of Oncidium 'Gower Ramsey'. Plant Cell, Tissue and Organ Culture, 77: 107-109.

Zahara, M. 2017. 'A review: Micropropagation of Phalaenopsis sp. from leaf and flower stalk explants'. Jurnal Natural, 17 (2): 91-95.

Zhao, P., W. Wang, S. F. Feng, F. Wu, J. Q. Yang and W. J. Wang. 2007. 'High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium candidum Wall ex Lind.' Plant Cell, Tissue and Organ Culture, 90: 131–139.