Ameliorative properties of methanol leaf extract of Momordica charantia following alloxan-induced cardiotoxicity in rats

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 8، دوره 10، شماره 1، مرداد 2019، صفحه 61-72   

نوع مقاله: Original article

نویسندگان

Sunday Ofuegbe¹؛ Ademola Oyagbemi²؛ Temidayo Omobowale³؛ Aduragbenro Adedapo⁴؛ Abiodun Ayodele⁵؛ Momoh Yakubu⁶؛ Oluwafemi Oguntibeju⁷؛ Adeolu Adedapo^{* 8}

¹Department of Veterinary Pharmacology and Toxicology University of Ibadan, Nigeria

²Department of Veterinary Physiology and Biochemistry University of Ibadan Ibadan, Nigeria

³Department of Veterinary Medicine University of Ibadan Ibadan, Nigeria

⁴Department of Pharmacology and Therapeutics University of Ibadan Ibadan, Nigeria

⁵Department of Botany University of Ibadan Ibadan, Nigeria

⁶Department of Environmental & Interdisciplinary Sciences, College of Science, Engineering & Technology, Texas Southern University, Houston, TX, USA

⁷Department of Biomedical Sciences Cape Peninsula University of Technology Bellsville, South Africa.

⁸Department of Veterinary Pharmacology and Toxicology Faculty of Veterinary Medicine University of Ibadan

چکیده

Background & Aim:Momordica charantiais an extensively distributed plant that is broadly prescribed in African medical system for treatment of various ailments. The plant has a comprehensive range of therapeutic uses. The present study reveals the antioxidative and cardioprotective abilities of methanol leaf extract of Momordica charantia (MLEMC) against cardiotoxicity using alloxan-induced animal model.
Experimental: The ameliorative effect of the methanol leaf extract of Momordica charantia (MLEMC) was studied in alloxan-induced cardiac injury in 50 rats divided into five groups (A-E) (n =10) i.e. group A control, group B was toxicant group, group C animals received glibenclamide treatment while groups D and E received extracts at 200 and 400 mg/kg doses, respectively, for 28 days. Histopathological changes, serum cardiac injury markers such as myeloperoxidase (MPO) activity, nitric oxide (NO) contents; oxidative status, blood pressure, electrocardiogram, cardiac P38 and CRP were evaluated.
Results: The extract-treated group showed a decreased level of oxidant markers such as malondialdehyde(MDA) and hydrogen peroxide(H₂O₂) but increased levels of protein thiols, non-protein thiols, glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. Heart sections revealed mild distortion of the cardiac architecture compared to toxicant group while decreased expression of cardiac P38 and CRP in extract-treated groups was observed.
Recommended applications/industries: The plant extract exhibited anti-oxidant and anti-inflammatory effects, thereby displaying cardio-protective property which propose the plant as a good natural source for herbal nutraceuticals.

کلیدواژه‌ها

Momordica charantia؛ Cardiotoxicity؛ Diabetes؛ Oxidative stress؛ Immunohistochemistry

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