The Role of signaling of hydrogen peroxide and 24-epibrassinosteroid on physiological traits of cumin (Cuminum cyminum L.) under drought stress

Iranian Journal of Plant Physiology

مقاله 5، دوره 10، شماره 3، تیر 2020، صفحه 3243-3254   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2020.1893417.1204

نویسندگان

Nasim Roudbari¹؛ Hosein Abbaspour^{* 2}؛ Khosrow Kalantari³؛ Ahmad Aien⁴

¹Department of biology, Faculty member of Kahnouj Branch, Islamic Azad University

²Department of biology, North Tehran Branch, Islamic Azad University, Tehran

³Department of Biology, Shahid bahonar University of kerman

⁴Seed and Plant Improvement Department, South Kerman Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Jiroft, Iran

چکیده

Two biochemical compounds of hydrogen peroxide and 24-epibrassinosteroid have significant biological effects on plant growth. In the present study, the effects of drought and its interaction with H2O2 and 24-epibrassinosteroid on the protein, sugars, essential oil percentage, photosynthetic pigments, phenols and flavonoids were investigated. For this purpose, a factorial experiment in a completely randomized design with three replications was conducted in a greenhouse of Agricultural Research. Plants were planted in pots. In the fifth week after germination, plants were treated under drought stress at three levels: 100% (control), 75% and 50% field capacity. The plants were sprayed with hydrogen peroxide and 24-epibrassinosteroid at concentrations of 0, 0.5 and 1 mM, sequentially in two steps: three days before stress and 15 days later. The results showed main effects of drought stress and spraying had a significant effect on the studied traits, so that with increasing stress level, the essential oil percentage, soluble sugars, carotenoids, flavonoids of the shoot increased, and the shoot protein and chlorophyll contents decreased. With increasing levels of H2O2 and 24-epibrassinosteroid, the contents of chlorophyll a and b increased, so that by spraying with 1 mM 24-epibrassinosteroid, chlorophyll a reached the highest level (10.90 mg mL-1). Spraying 24-epibrassinosteroid also increased the shoot phenol content (1.58 Obsarbance g) and decreased essential oil percentage (2.44%) under severe stress. The results showed hydrogen peroxide and 24-epibrassinosteroid acted as signal molecules at optimum concentrations and allowed the cumin plant to adapt to drought conditions by reducing membrane peroxidation and inducing physiological activities.

کلیدواژه‌ها

Epibrassinosteroid؛ Hydrogen peroxide؛ Protein؛ Drought stress؛ Cumin

مراجع

Abd, El-Monem M. I., I. F. Sharaf and R. S Mahmoud. 2009. 'Role of gibberellic acid in abolishing the detrimental effects of Cd and Pb on broad bean and lupin plants'. Research Journal of Agriculture and Biological Sciences. 5(5): 668-673.

Ahmadi Mousavi, E., Kh. Manoukhehri Kalantari and M. Torkzadeh. 2005. 'Effects of 24-epibrassinolide on lipid peroxidation, prolin, sugar and photosynthesis pigments content of colza (Brassica napus L.) under water stress'. Iranian Journal of Botany. 18(4): 295-306. (In Persian).

Ali, Q., M. Ashraf and H.R Athar. 2007a. 'Exognously applied proline at different growth stages enhances growth of two maize cultivars grown under water deficit conditions'. Pakistan Journal of Botany. 39: 1133-1144.

Ali, B., S. Hayat and A. Ahmad. 2007b. '28-Homobrassinolide ameliorates the saline stress in chickpea (Cicer arietinum L).' Environmental and Experimental Botany. 59: 217–223.

Baghizadeh, A., M. Ghorbanli, H.M Rezaei, and H. Mozafri, 2009. 'Evaluation of Interaction effect of drought stress with ascorbate and salicylic acid on some of physiological and Biochemical parameters in okra (Hibiscus esculentus L.)'. Journal Biological sciences. 4 (4): 380-387. (In Persian).

Bahraminejad, A., Gh. Mohammadi-Nejad and M. Khadir. 2011. 'Genetic diversity evaluation of Cumin (Cumin cyminum L.)based on phenotypic traits'. Aust. J. Crop Sci. 5: 301-307.

Bakhshi S., H. Abbaspour and S. Saeidisar. 2018. 'Study of phytochemical changes, enzymatic and antioxidant activity of two halophyte plants: Salsola dendroides Pall and Limonium reniforme (Girard) Lincz in different seasons'. Journal of Plant Environmental Physiology, 46: 79-92. (In Persian).

Bienert, G. P., J. K. Schjoerring and T. P Jahn. 2006. 'Membrane transport of hydrogen peroxide'. Biochemistry and Biophysic Acta, 1758, 994-1003.

Bradford, M. M, 1976. 'A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein–dye binding'. Analytical Biochemistry 72: 248-254.

Debnath, M., N. Ashwath and D.J.  Midmore. 2019. 'Physiological and morphological responses to abiotic stresses in two cultivars of Stevia rebaudiana (Bert.) Bertoni'. South African Journal of Botany. 123: 124-132.

Daniel, Z., and H. Maria. 2002. 'Domestication of plants in the old world'. Third ed. University Press, Oxford. p. 206.

Fariduddin, Q., M. Yusuf., S. Chalkoo., S. Hayat, and A. Ahmad. 2011. '28-homobrassinolide improves growth and photosynthesis in Cucumis sativus L. through an enhanced antioxidant system in the presence of chilling stress'. Photosynthetica. 49: 55-6.

Farooq, M., A. Wahid., N. Kobayashi., D. Fujita, and S. M. A. Basra. 2009. 'Plant drought stress: effects, mechanisms and management'. Agronomy for Sustainable Development. 29(1): 185-212.

Gohari, A. R., H Hajimehdipoor, S Saeidnia, Y Ajani, A Hadjiakhoondi. 2011. 'Antioxidant activity of some medicinal species using FRAP assay'. Medicinal Plants.10: 54-60.

Hung, S-H., C-W. Yu and CH. Lin. 2011.' Hydrogen peroxide function as a stress signal in plants'. Bot Bull Acad Sin, 46: 1-10.

Izadi, Z., M. Esna ashari and G.  Ahmadvand.2010. 'Effect of drought stress on Yield, Proline contents, Soluble sugars, Chlorophyll, Relative water contents and essential oil in peppermint (Mentha piperita L.).' Iranian journal of  horticultural science and technology. 10(3). 223-234. (In Persian).

Jaleel, C., P. Manivannan., G. Lakshmanan., M. Gomathinayagam and R. Panneerselvam. 2008. 'Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits'. Colloids and Surfaces B: Biointerfaces. 61(2): 298-303.

Kafi, M. 2002. 'Cumin (Cuminum cyminum) production and processing'. Ferdowsi University of Mashhad Press, Mashhad, 168p.

Kafi, M., M.H. Rashed-Mohassel., A. Koocheki and A. Mlafylaby. 2003. 'Technology, production and processing cumin. Publications Excellence in Special Crops'. Ferdowsi University of Mashhad. Press, 195p. (In Persian).

Khan, N.A., R. Nazar., N. Iqbal and N.A. Anjum. 2012. 'Phytohormones and abiotic stress tolerance in plants'. Springer-Verlag Berlin Heidelberg. 311p.

Kochert, G. 1978. 'Carbohydrate determination by the phenol sulfuric acid method'. In: Helebust.J.A. Craig J.s.(ed): Hand book of phycological Method 56-97. Cambridge Univ. Press. Cambridge.

Krasensky, J and C. Jonak. 2012. 'Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks'. Journal of Experimental Botany. 63:1593-1608.

Li, J.T., Z.B. Qiu and X.W. Zhang, 2011. 'Exogenous hydrogen peroxide can enhance tolerance of wheat seedlings to salt stress'. Acta Physiology Plant. 33: 835-842.

Lichtenthaler, H.K. 1992. 'The Kaustky effect: 60 years of chlorophyll fluorescence induction kinetics'. Food Crops to Temperature and Water Stress, AVRDC, Shanhua, Taiwan, pp: 389- 398.

Liu, ZJ., XL. Zhang., JG. Bai., BX. Suo., PL. Xu and L. Wang. 2010. 'Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves'. Sci Hortic. 121:138–143.

Lotfallahi, L., H. Torabi. and H. Omidi.  2016. 'Salinity effect on proline, photosynthetic pigments and leaf relative water content of chamomile (Matricaria chamomilla L.) Inhydroponic condition'. Journal of  plant production (Journal of agricultural sciences and natural resources).  22(1): 89-104. (In Persian).

Lum M S, M. M. Hanafi., Y M. Rafii and A. S. N Akmar, 2014. 'Effect of drought stress on growth, proline and antioxidant enzyme activities of upland rice'. J Anim Plant Sci. 24(5): 1487–1493.

Lawlor, D.W. 2002.' Limitation to photosynthesis in water stressed leaves: stomata versus metabolism and the role of ATP'. Annals of Botany. 89. 1-15.

Mao LC., HG. Pang., GZ. Wang and CG. Zhu. 2007. 'Phospholipase D and lipoxygenase activity of cucumber fruit in response to chilling stress'. Postharvest Biol. Technol. 44:42–47.

Matta, A.J. and I. Giai, 1969. 'Accumulation of phenol in tomato plant in effected by different forms of Fusarium oxysporum'. Planta, 50(1): 512-513.

Massacci, A., S. Nabiev, L. Pietrosanti, S. Nematov, T. Chernikova, K. Thor, and J. Leipner, 2008. 'Response of the photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas exchange analysis and chlorophyll fluorescence imaging'. Plant Physiology and Biochemistry. 46(2):189-195.

Matsouka, I., D. Beri., I. Chinou., K. G.  Haralampidis and C. Spyropoulos. 2011. 'Metals and selenium induce medicarpin accumulation and excretion from the roots of fenugreek seedlings: a potential detoxification mechanism'. Plant Soil Published Online.

Navabpour, S., S. S. Ramezanpour and A. Mazandarani. 2016. 'Evaluation of enzymatic
and non-enzymatic defense mechanism in response to drought stress during growth
 tage in soybean'. Plant Production Technology. 7(2): 39-54. (In Persian).

Nobakht, P., A. Ebadi., Gh. Parmoon., Nickhah Bahrami, 2019.'Study role H₂O₂ in Photosynthetic pigments Peppermint (Mentha piperita L) on Water stress conditions'. Journalof Plant Process and function iranin society of plant physiology, 7(27): 19-30. (In Persian).

Nogues, S. and N.R. Baker. 2000. 'Effects of drought on photosynthesis in Mediterranean plants grown under enhanced UV_B radiation'. Journal of Experimental botany, 51 (348): 1309- 1317.

Parvaiz, A. and S. Satyawati. 2008. 'Salt stress and Phyto-biochemical responses of plants'. Plant Soil Environment, 54: 89-99.

Peleg Z. and E. Blumwald. 2011. 'Hormone balance and abiotic stress tolerance in crop plants'. Curr Opin Plant Biol 14: 290–295.

Pustovoitova, T.N., N. E. Zhdonova. and V.N.   Zholkevich. 2000. 'Epibrassinolide increase plant drought resistance'. Doklady Biochemistry and Biophysics. 376: 36-38.

Quan, L. J., B. Zhang, W.W. Shi and H.Y. Li, 2008. 'Hydrogen peroxide in plants: A versatile molecule of the reactive oxygen species network'. J. Integr. Plant Biol, 50: 2-18.

Rassam, Gh., A. Dadkhah., A. Khoshnood yazdi and M.  Dashti. 2015.' Impact of Humic Acid on Yield and Quality of Sugar Beet (Beta vulgaris L.) Grown on Calcareous Soil'. Notulae Sci Biol, 7(3):367-371. (In Persian).

Safikhani, F., H. Heydari sharifabad A. Syadat,.  A. Sharifi ashorabadi., M. Syednedjad and B. Abbaszadeh. 2007. 'The effect of drought stress on percentage and yield of essential oil and physiological characteristics of Deracocephalum moldavica L.' Iranian Journal of Medicinal and Aromatic   Plants. 23(1): 86-99. (in Persian).

Salimi, F., F. Shekari and J. Hamzei. 2016.' Methyl jasmonate improves salinity resistance in German chamomile (Matricaria chamomilla L.) by increasing activity of antioxidant enzymes', Acta Physiologiae Plantarum. 38(1).

Seif, S. N., E. Tafazzoli.,  A. R., A. Aboutalebi and V. Abdosi,2014. 'Evaluation of two grape cultivars (Vitis vinifera L.) against salinity stress and surveying the effect of methyl jasmonate and epibrassinolide on alleviation the salinity stress'. International Journal of Biosciences (IJB). 7 :116-125.

Shah, S.H, 2007.' Effects of salt stress on mustard as affected by gibberellic acid application. Gen. Appl. Plant physiology. 33(1-2): 97-106.

 

Shahin, M.F.M., M.I.F. Fawzi and E.A. kandil, 2010. Influence of foliar application of some nutrient (Fertifol Misr) and gibberellic acid on fruit set, yield, fruit quality and leaf composition of “Anna” Apple trees grown in sandy soil'. Journal of American Science. 6(12): 202-208.

Tasgin, E., O. Atici, and B. Nalbantoglu, 2006. 'Effects of salicylic acid and cold on freezing Tolerance in winter wheat leaves.' Plant Growth Regulation. 41:231- 236.

Saffari, M., J. Ahmadi., N. A. Khoshkholgh Sima., Z. Shobbar. and MR. Naghavi, 2013. 'The Evaluation of Cytokinins and Brassinosteroid Hormones spray effects on drought tolerance gene expression profile in tolerant and susceptible canola cultivars'. Dissertation, University of International Imam Khomeini, Iran.

Strauss, G. and H. Hauser, 1986. 'Stabilization of mlipid bilayer vesicles by sucrose during freezing'. Proc. Natl. Acad. Sci. U.S.A. 83: 2422-2426.

Wang, D., Q. Shi., X. Wang., M. Wei., J. Hu., J. Liu, J. and F. Yang, 2010. 'Influence of cow manure vermicompost on the growth, metabolite contents, and antioxidant activities of Chinese cabbage (Brassica campestris ssp. chinensis)'. Biology and fertility of soils, 46: 689-696.