Antidiabetic and anti-oxidant effects of methanol leaf extract of Momordica charantia following alloxan-induced hyperglycaemia in rats

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

دوره 10، شماره 3، آبان 2019، صفحه 95-108   

نوع مقاله: Original article

نویسندگان

Sunday Ofuegbe¹؛ Olufunke Falayi¹؛ Blessing Ogunpolu²؛ Ademola Oyagbemi³؛ Temidayo Omobowale²؛ Momoh Yakubu⁴؛ Oluwafemi Oguntibeju⁵؛ Adeolu Alex Adedapo^{* 6}

¹Department of Veterinary Pharmacology and Toxicology, University of Ibadan, Nigeria;

²Department of Veterinary Medicine, University of Ibadan, Nigeria;

³Department of Veterinary Physiology and Biochemistry, University of Ibadan, Nigeria;

⁴Department of Environmental and Interdisciplinary Sciences, COSET, Texas Southern University, Houston, TX, USA;

⁵Department of Biomedical Sciences, Cape Peninsula University of Technology, Bellsville, South Africa;

⁶Department of Veterinary Pharmacology and Toxicology Faculty of Veterinary Medicine University of Ibadan

چکیده

Background & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats.
Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated.
Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group.
Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus.

کلیدواژه‌ها

Diabetes mellitus؛ Momordica charantia؛ Alloxan؛ Hydrogen peroxide؛ Malondialdehyde؛ Superoxide dismutase؛ Glutathione peroxidase

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