Titanium Dioxide Nanoparticles Increase Resistance of L. iberica to Drought Stress due to Increased Accumulation of Protective Antioxidants

Iranian Journal of Plant Physiology

دوره 10، شماره 4، آبان 2020، صفحه 3343-3354   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2020.1901976.1233

نویسندگان

Niloufar Shoarian¹؛ Rashid Jamei^{* 2}؛ Bahman Pasban Eslam³؛ Seyed Yahya Salehi Lisar⁴

¹Faculty of Natural Science, Urmia University International Pardis, Urmia, Iran

²Faculty of Natural Sciences, Urmia University, Urmia, Iran

³Crop and Horticultural Science Research Department, East Azarbaijan Agricultural and Natural Resources Research and Education Center, AREEO, Tabriz, Iran

⁴Biology Group Adress: Faculty of Natural Sciences,Tabriz University, Tabriz , Iran

چکیده

This research was carried out to study the effect of TiO2 and nano-TiO2 on some chemical compounds in a medicinal plant Lallemantia iberica under water deficit conditions. The experiment was conducted as a factorial arrangement in a randomized complete block design with three replications. The first factor was the application of external materials as spraying with 3 levels of control, TiO2 and nano-TiO2, and the second factor was 100% (no stress), 75% (moderate stress) and 35% (severe stress) irrigation content. The results of measurements showed moderate stress and the use of nano-TiO2 caused a significant increase in phenolic content, total flavonoid and antioxidant activity; severe drought stress significantly reducing these compounds. The effect of nano-TiO2 on catalase and superoxide dismutase enzyme activity was also significant, and in the treatment of nano-TiO2 and moderate stress, the highest activity level of these two enzymes was observed. Identification of phenolic compounds of the extracts by HPLC indicated the production of vanillic, ferulic acid and syringic acid by nano-TiO2 spray application. Gallic acid was measurable in moderate and severe stress and caffeic acid in severe stress under the influence of nTiO2. The sinapic acid and syringic acid contents increased under moderate stress and severe stress decreased them. Finally, it is concluded that nano-TiO2 in comparison with TiO2 reduces the effects of drought stress on L. iberica and can be used to produce this plant in water-deficit areas.

کلیدواژه‌ها

antioxidant activity؛ Lallemantia iberica؛ nano-TiO2؛ phenolic compounds؛ drought stress