نقش سترورلیکس در حفاظت از تغییرات مورفولوژیکی و فراساختاری اپی‌تلیوم ژرمینال بافت بیضه به دنبال آسیب‌های ایجادشده توسط سیکلوفسفامید در موش سوری

آسیب‌شناسی درمانگاهی دامپزشکی

مقاله 7، دوره 14، 3 (55) پاییز، آبان 1399، صفحه 285-299   

نوع مقاله: مقاله علمی پژوهشی

شناسه دیجیتال (DOI): 10.30495/jvcp.2020.1908974.1279

نویسندگان

داریوش محمدنژاد¹؛ جمال عیوضی ضیایی²؛ ایدا اعظمی³؛ محمدرضا ولیلو⁴؛ حسین راستا⁵؛ علی عابدالهی^{* 2}

¹دانشیار مرکز تحقیقات کاربردی دارویی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.

²دانشیار مرکز تحقیقات هماتولوژی و انکولوژی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.

³کارشناس ارشد مرکز تحقیقات کاربردی دارویی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.

⁴استادیار گروه پاتوبیولوژی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

⁵دانشیارگروه فیزیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز، ایران.

چکیده

داروهای مورد استفاده در درمان سرطان‌ها، اثرات مضر در ارگان‌های با تقسیمات سریع سلولی از جمله بافت بیضه دارند. آنتاگونیست­های گنادوتروپین با مهار تقسیم در سلول‌های اسپرماتوژنیک باعث حفاظت سلول‌های مذکور از عوارض داروهای شیمی درمانی از جمله سیکلوفسفامید می‌گردند. هدف از انجام این مطالعه، بررسی توانایی سترورلیکس (آنتاگونیست گنادوتروپین)، در مهار اثرات سوء سیکلوفسفامید در بافت بیضه موش سوری بود. بدین منظور 30 سر موش سوری بالغ نر 8-6 هفته­ای به 3 گروه کنترل (هیچ دارویی را دریافت نکردند)، آزمایش 1 (دریافت­کنندهmg/kg ۵۰وزن بدن،سیکلوفسفامید به‌صورت داخل صفاقی) و آزمایش 2 (دریافت­کننده­mg/kg  ۲۵/۰ وزن بدن،سترورلیکس به­صورت زیرجلدی به همراه سیکلوفسفامید به‌میزانmg/kg   ۵۰ وزن بدن) تقسیم شدند. 35 روز بعد از آخرین تزریق سترورلیکس، همه موش‌ها تحت بیهوشی کشته شده و بیضه‌ آن­ها برای مطالعات هیستومورفومتریک و فراساختاری برداشته شد. مطالعه لوله‌های سمینی‌فر با میکروسکوپ نوری نشان­دهنده کاهش شدید سلول‌های اسپرماتوژنیک و ضخامت اپی‌تلیوم ژرمینال و کاهش معنی‌دار میانگین شاخص اسپرمیوژنز (spermiogenesis index; SI) و تعداد سلول‌های سرتولی در گروه آزمایش 1 بود (05/0p < /em><). مطالعه میکروسکوپ الکترونی نیز نشان دهنده آسیب به سلول‌های سرتولی و پیدایش فضاهای وسیع و متعددی در بین این سلول‌ها و سلول‌های اسپرماتوژنیک بود. از طرف دیگر در سلول‌های سرتولی، میتوکندری­های در حالت تخریب قابل تشخیص بودند. اما در گروه آزمایش 2، شرایط تا اندازه­ای شبیه گروه کنترل بود. نتایج مطالعه حاضر نشان داد که سترورلیکس می‌تواند اپی‌تلیوم ژرمینال را تا حدودی از اثرات سوء سیکلوفسفامید حفاظت کند.

کلیدواژه‌ها

بیضه؛ سیکلوفسفامید؛ سترورلیکس؛ موش سوری

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