Anti-hepatofibrotic effect of ethyl acetate fraction of Bombax costatum Pellgr.EtVuillet stem bark against CCl4-induced liver fibrosis in mice

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 1، دوره 12، شماره 1، مرداد 2021، صفحه 1-7   

نوع مقاله: Original article

شناسه دیجیتال (DOI): 10.30495/medherb.2021.679007

نویسندگان

Nuhu Mohammed^{* 1}؛ Joseph Anuka¹؛ Aliyu Muhammad Musa²؛ Jamilu Yau¹

¹Department of pharmacology and therapeutics, Faculty of pharmaceutical sciences, Ahmadu Bello University, Zaria, Nigeria.

²Department of Pharmaceutical and Medicinal Chemistry, Ahmadu Bello University Zaria, Nigeria;

چکیده

Background & Aim: Bombax costatum stem bark is traditionally used in treatment of liver diseases but the anti-hepatofibrotic effect of its ethyl acetate fraction has not been scientifically evaluated. This study aimed to evaluate the anti-hepatofibrotic effect of ethyl acetate fraction of B. costatum stem bark (EAB) against carbon tetrachloride (CCl₄) induced liver fibrosis in mice.
Experimental: Normal control group were administered olive oil while mice in the remaining groups received 0.4 ml/kg of CCl₄ twice weekly for 6 weeks. Mice in treatment groups received EAB once daily orally for the next 2 weeks at doses of 31.25, 62.5 and 125 mg/kg body weight, respectively. Mice in standard control group received silymarin (100 mg/kg) daily for the next 2 weeks. Mice in toxic control group were sacrificed 72 hours after the last dose of CCl₄ while mice in CCl₄ control group were observed for the next 2 weeks for spontaneous resolution of fibrosis. Effect of treatment on tumor necrosis factor-α (TNFα), transforming growth factor-β1 (TGFβ1), malondialdehyde (MDA), reduced glutathione (GSH) levels and liver histopathology were evaluated.
Results: CCl₄ intoxication caused significant (p < 0.05) elevation in TNFα, TGFβ1 and MDA with associated collagen deposition in the liver. Administration of EAB significantly (p < 0.05) decreased the level of TNFα, TGFβ1 as well as MDA and increased GSH level compared to CCl₄ toxic group.
Recommended applications/industries: Ethyl acetate fraction of B. costatum possesses anti-hepatofibrotic, anti-inflammatory and in vivo anti-oxidant activities against CCl₄ induced liver fibrosis.

کلیدواژه‌ها

Bombax costatum؛ Carbon tetrachloride؛ Ethyl acetate؛ Fibrosis؛ Mice

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