تغییرات شاخص‌های جوانه‌زنی و فعالیت سیستم آنتی‌اکسیدانتی بذرهای پنبه (Gossypium hirsutum L.) طی زوال

فیزیولوژی محیطی گیاهی

دوره 16، شماره 62، تیر 1400، صفحه 35-49   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2021.679513

نویسندگان

فرشید قادری‌فر^{* 1}؛ امید سنچولی¹؛ حمیدرضا صادقی پور²

¹گروه زراعت، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

²گروه زیست‌شناسی، دانشگاه گلستان، گرگان، ایران

چکیده

بذرها در طی انبارداری زوال یافته و پیر می­شوند به طوری که سرعت  این فرآیند به دما و رطوبت بذر در طی انبارداری بستگی دارد. این آزمایش به منظور مطالعه فعالیت سیستم آنتی اکسیدانتی در بذرهای پنبه رقم ارمغان در طی زوال صورت گرفت. برای ایجاد سطوح مختلف زوال از آزمون تسریع پیری استفاده شد. بذرهای پنبه به‌مدت 0، 48، 96، 144 و192 ساعت در دمای 43 درجه سانتی­گراد و رطوبت نسبی 100 درصد قرار گرفتند. نتایج نشان داد با افزایش دوره زوال هدایت الکتریکی غشاء، پراکسیداسیون لیپید و پراکسید هیدروژن افزایش یافت. افزایش پراکسید هیدروژن همراه با کاهش فعالیت آنزیم کاتالاز و افزایش فعالیت آنزیم پراکسیداز و مقدار اسید آسکوربیک بود که نشاندهنده تاثیر کاهنده دوره زوال بر فعالیت آنزیم کاتالاز نسبت به آنزیم پراکسیداز و اسید آسکوربیک بود. همچنین با افزایش دوره زوال درصد، سرعت و یکنواختی جوانه­زنی کاهش یافت. به­طور کلی نتایج این تحقیق بیانگر این مطلب است که یکی از دلایل اصلی زوال بذر در بذرهای پنبه، تنش اکسیداتیو ناشی از تجمع گونه­های اکسیژن فعال و کاهش کارایی سیستم آنتی اکسیدانتی می ­باشد.

کلیدواژه‌ها

انبارداری بذر؛ پیری؛ قدرت بذر؛ تنش اکسیداتیو؛ گونه های اکسیژن فعال

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