بررسی اثر کود نانوکلات پتاسیم، سولفات پتاسیم و سالیسیلیک اسید بر خصوصیات فیزیولوژیکی گیاه همیشه بهار (Calendula officinalis L.) تحت تنش کمبود آب

فیزیولوژی محیطی گیاهی

دوره 17، شماره 65، فروردین 1401، صفحه 66-85   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2021.679554

نویسندگان

سمیه عرفانی¹؛ محمد علی رضایی^{* 1}؛ فرهادفرهاد فرح‌وش²؛ مهرعلی محمود جانلو¹

¹گروه زیست شناسی، دانشکده علوم پایه، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

²گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران

چکیده

کمبود آب از جمله عوامل محدودکننده رشد می‌باشد و تنش اکسیداتیو به عنوان یک تنش ثانویه در نتیجه تنش خشکی و کم آبی بوجود می‌آید. به منظور بررسی سطوح کودهای نانوکلات پتاسیم، سولفات پتاسیم و سالیسیک اسید در کاهش اثرات کم آبی بر تعدادی شاخص های فیزیولوژیکی گیاه همیشه‌بهار آزمایشی به صورت فاکتوریل بر پایه طرح کاملا تصادفی با سه تکرار در سال 1398 در گلخانه تحقیقاتی دانشگاه آزاد اسلامی واحد گرگان انجام شد. عامل‌های مورد بررسی شامل آبیاری در دو سطح ، مصرف کود پتاسیم در دو سطح نانوکلات پتاسیم و سولفات پتاسیم، و سالیسیلک اسید در سه سطح صفر، یک و دو میلی مولار بودند. صفات فیزیولوژیکی از قبیل رنگیزه‌های فتوسنتزی، پرولین، فنل کل، آنزیم سوپراکسیداز، آنزیم کاتالاز، قند محلول، پروتئین و گلایسین بتائین مورد ارزیابی قرار گرفت. برپایه نتایج بدست آمده از این پژوهش تنش کم‌آبی به طور معنی داری میزان پرولین، آنزیم پراکسیداز، آنزیم کاتالاز، گلایسین بتائین و قند را افزایش داده و میزان کلروفیل a، b، کل برگ و پروتئین کل را کاهش داد. کاربرد برگی اسیدسالیسیلک در شرایط تنش کم آبی در غلظت به طور معنی‌داری باعث افزایش قند، پروتئین کل، گلایسین بتائین و فنل کل شد. کاربرد نانوکلات پتاسیم و سولفات پتاسیم باعث بهبود تحمل گیاه در شرایط تنش خشکی شد و موجب افزایش صفات فیزیولوژیکی نسبت به شاهد شد.

کلیدواژه‌ها

پرولین؛ رنگیزه‌های فتوسنتزی؛ کاتالاز؛ گلایسین بتائین؛ همیشه‌بهار

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