تاثیر نانو ذرات نقره‌ سنتز شده زیستی بر روی برخی از شاخص‌های فیزیولوژیکی و بیوشیمیایی بنفشه سه رنگ (Viola tricolor L.)

فیزیولوژی محیطی گیاهی

دوره 16، شماره 62، تیر 1400، صفحه 109-122   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2021.679557

نویسندگان

عارفه حسنوند¹؛ سارا سعادتمند^{* 1}؛ حسین لاری یزدی²؛ علیرضا ایرانبخش¹

¹گروه زیست شناسی ، دانشکده علوم پایه، واحد علوم وتحقیقات ،دانشگاه آزاداسلامی،تهران،ایران

²گروه زیست شناسی ، دانشکده علوم پایه ،واحد بروجرد ،دانشگاه آزاداسلامی، بروجرد،ایران

چکیده

استفاده از نانوذرات زیستی نقش مهمی در افزایش بهره وری کشاورزی ایفا می­کند. در سال­های اخیر استفاده از نانوذرات در گیاهان به ­عنوان آفت­کش­ها، عوامل حفاظتی و مواد مغذی مورد توجه قرار گرفته است. بنفشه سه رنگ گیاه دارویی از خانواده ویولاسه است که دارای خواص دارویی ضد باکتریایی، ضد قارچی وضد سرطانی است. در این پژوهش نانوذره­ی نقره به­وسیله نیترات ­نقره و عصاره گیاه بنفشه سه رنگ زیست سنتز شد و سپس اثر غلظت­های مختلف نانوذرات نقره بر برخی شاخص­های فیزیولوژیکی و بیوشیمیایی گیاه بنفشه سه رنگ مطالعه گردید. نتایج نشان داد شاخص­های مختلف رشد از جمله وزن­تر ریشه و ساقه، طول ریشه و ساقه به­طور معنی­داری تحت تاثیر نانوذرات نقره افزایش یافت، به­طوری‌که بیشترین میزان این شاخص­ها به ترتیب در غلظت­های 0، 10، 50 و 100 میلی­گرم در لیتر نانوذرات نقره مشاهده شد. همچنین میزان پرولین و کربوهیدرات تحت تاثیر غلظت­های نانوذرات نقره در مقایسه با شاهد افزایش نشان داد که بیشترین مقدار این شاخص­هادر 100میلی­گرم در لیتر نانوذرات نقره مشاهده شد. . میزان متابولیت­های ثانوی از جمله فنل و فلاونوئید تحت تاثیر نانوذرات نقره در غلظت 100 میلی­گرم در لیتر بودند که با بیشترین افزایش همراه بود. حداکثر افزایش میزان آنتوسیانین تحت تاثیر غلظت 10 میلی­گرم در لیتر نانوذرات نقره بود. نتایج بررسی فعالیت آنزیم­های آنتی‌اکسیدان نشان داد، در تمام غلظت­های نانوذرات نقره، فعالیت آنزیم­های آنتی­اکسیدانی افزایش یافته است. افزایش فعالیت آنزیم­های آنتی اکسیدانی (کاتالاز، گلوتاتیون­ ردوکتاز) تحت تاثیر نانوذرات نقره منجر به کاهش فعالیت محتوای مالون دی­آلدئید  شد. براساس نتایج این تحقیق، نانوذرات نقره به عنوان محرکی مناسب جهت افزایش رشد و تولید میزان صفات آنتی­ اکسیدانی پیشنهاد می­شود.

کلیدواژه‌ها

آنتوسیانین؛ رشد ریشه؛ کاتالاز؛ گلوتاتیون‌ردوکتاز؛ مالون دی‌آلدئید

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