اثر تنظیم کننده پیکس بر پارامترهای رشد آنزیم‌های آنتی اکسیدانی و شاخص‌های اکسیدانی دانه رست دو رقم ساحل و شایان گیاه رست پنبه (Gossypium spp.) در سطوح مختلف خشکی

فیزیولوژی محیطی گیاهی

مقاله 3، دوره 15، شماره 60، دی 1399، صفحه 32-47   

نوع مقاله: پژوهشی

نویسندگان

شهربانو غفوری¹؛ مریم نیاکان^{* 1}؛ عمران عالیشاه²؛ محمدرضا زنگی²

¹گروه زیست‌شناسی، واحد گرگان، دانشگاه آزاد اسلامی، گرگان، ایران

²موسسه تحقیقات پنبه کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، گرگان، ایران

چکیده

مپی کوات کلرید (پیکس) به عنوان یک ابزار مهم مدیریتی برای کاهش آسیب تنش در گیاه پنبه در مرحله جوانه‌زنی می‌باشد. در این تحقیق، اثر سطوح مختلف خشکی و میزان مپی کوات کلراید (پیکس) بر شاخص‌های رشد و آنزیم‌های آنتی اکسیدانی و نیز میزان مالون دی آلدهید و پراکسید هیدروژن دانه رست دو رقم پنبه تحت شرایط آزمایشگاهی بررسی شد. این تحقیق در یک طرح کاملاً تصادفی با استفاده از غلظت‌های 0 ، 5 و 10 گرم بر لیتر پیکس و نیز اعمال سطوح خشکی با کاربرد پلی اتیلن گلیکول6000 در مقادیر 0، 4- و 8- بار در ظروف پتری بر دو رقم ساحل (حساس به خشکی) و شایان (مقاومت به خشکی) گیاه پنبه انجام شد. شاخص‌های جوانه زنی و نیز پارامترهای فیزیولوژیکی دانه رست‌های تحت تیمار خشکی و پیکس پس از 4 روز اندازه گیری شد. نتایج نشان داد که استفاده از پیکس باعث افزایش درصد جوانه زنی ، طول ریشه‌چـه، وزن خشک و تردانه رست و فعالیت آنزیم‌های پراکسیداز و کاتالاز ارقام پنبه در شرایط تنش خشکی شد در حالیکه اعمال تنش خشکی به تنهایی موجب کاهش پارامترهای ذکر شده گشت. همچنین اعمال خشکی موجب افزایش شاخص‌های اکسیدانی یعنی مالون دی آلدهید و پراکسید هیدروژن در دانه رست‌های دو رقم ساحل و شایان شد در حالی که کاربرد پیکس شاخص‌های نامبرده را کاهش داد. به‌طورکلی نتایج این تحقیق نشان داد کاربرد پیکس سبب بهبود پاسخ‌های رشدی و سیستم آنتی‌اکسیدانی دو رقم پنبه گشت که در این بین پاسخ رقم شایان مناسب‌تر از رقم ساحل بود.

کلیدواژه‌ها

آنزیم‌های آنتی اکسیدانی؛ پنبه؛ تنش؛ شاخص‌های مورفوفیزیولوژی؛ مپی کوات کلراید

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