Hydrolysis of fish waste using fruit wastes of Ananas comosus and Carica papaya for the formulation of liquid fertilizers

International Journal of Recycling Organic Waste in Agriculture

مقاله 3، دوره 10، شماره 2، شهریور 2021، صفحه 129-143   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30486/ijrowa.2021.1891960.1034

نویسندگان

RHAA Ranasinghe؛ BTSDP Kannagara^* ؛ RMCS Ratnayake

Department of Plant and Molecular Biology, Faculty of Science, University of Kelaniya, Sri Lanka

چکیده

Purpose Fish waste is a protein-rich source that can be used as a value-added product in the formulation of organic liquid fertilizers. This study carried out to investigate the utilizing potential of fruit wastes of Ananas comosus and Carica papaya that contain bromelain and papain as major proteases in order to hydrolyze fish waste proteins to fulfill plant nitrogen requirements.
 Method Proteases were extracted from A. comosus (leaves, crown, unripe fruit pulp, ripe fruit peels and pulp) and C. papaya (leaves, ripe fruit peels and unripe fruit peels). The optimum temperature and pH for the enzymatic activities were determined. Four liquid organic fertilizers were produced with hydrolyzed fish waste that enriched by adding Gliricidia sepium, Chromolaena odorata, Tithonia diversifolia, Mikania scandens and coconut husk-ash. Fertilizers were tested on the growth of Basella alba comparing with a standard fertilizer.
 Results Ananas comosus ripe fruit peels and the mixture of (A. comosus + C. papaya) showed the highest enzyme activities (0.33±0.02 and 0.36±0.01 U mL^-1 enzyme respectively) at 55 ^oC and 70 ^oC. The optimum pH for all the studied extracts was 7.5 at 37 ^oC. The highest plant fresh and dry weights were recorded in the foliar-applied fertilizer produced by hydrolyzing the fish waste using A. comosus and C. papaya, showing no significant differences to the standard fertilizer.
 Conclusion Hydrolysis of fish waste using the fruit wastes of A. comosus and C. papaya could be effectively used as an organic fertilizer for the growth of B. alba that leads towards sustainable waste management.

کلیدواژه‌ها

Ananas comosus ؛ Carica papaya؛ Fish wastes؛ Plant-derived proteases؛ Organic liquid fertilizers

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