Antibacterial activity and chemical profiling of Bombax glabrum (Pasq.) A. Robyns leaves

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

دوره 12، شماره 2، مهر 2021، صفحه 57-65   

نوع مقاله: Original article

شناسه دیجیتال (DOI): 10.30495/medherb.2021.684238

نویسندگان

Olufunmi A Whesu¹؛ Paul O Ogunbamowo^{* 2}؛ Samuel O Bankole³؛ Olamilekan Lanre Awotedu²؛ Rofiat I Oyediran⁴

¹Department of Pharmaceutical and Medicinal Chemistry, Olabisi Onabanjo University Ago-Iwoye, Nigeria;

²Biomedicinal Research Centre, Forestry Research Institute of Nigeria;

³Federal Polytechnic Ile-Oluji, Ondo State, Nigeria;

⁴Biotechnology Centre, Forestry Research Institute of Nigeria;

چکیده

Background & Aim:Bombax glabrum is used in traditional medicine for the relief of general pain and digestive problems. The aim of this study was to establish the antibacterial activity, to characterize and identify the bioactive compounds in the leaves of the plant.
Experimental: The powdered leave samples were sequentially extracted with n-hexane and chloroform using a soxhlet apparatus. Phytochemical screening was done using standard analytical procedures and the antibacterial activity of both extracts was tested against Bacillus subtills, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia using modified agar disc diffusion method. The chloroform extract was subjected to column chromatography separation in combination with TLC. Contact autobiography was carried out on two active spot(s) while purified fractions of these spots were analyzed using GC-MS.
Results: Phytochemical screening shows the presence of saponins, flavonoids, tannins, alkaloids, and sterols. The plant has inhibitory activity against S. aureus and E. coli; while the GC-MS of two active spots of the chloroform extract (with Rf of 0.60 and 0.82) yielded 12 and 17 compounds for band C3 and C5, respectively, which were active against S. aureus only. Most abundant compounds are bis (2-ethylhexyl) phthalate (34.54) and 2-pentadecanone, 6,10,14-trimethyl (41.81 %), respectively.
Recommended applications/industries: The presence of bioactive compounds could account for the plant’s inhibitory action against S. aureus and E. coli which may justify its use in managing infections

کلیدواژه‌ها

Bombax glabrum؛ GC-MS؛ antibacterial activity؛ TLC؛ Phytochemicals؛ column chromatography

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