تأثیر یک دوره تمرین هوازی-مقاومتی بر بیان ژن گیرنده‌ی فعال‌کننده پرولیفراسیون پروگسیزوم گاما بیماران بای‌پس عروق کرونری

فصلنامه زیست شناسی جانوری

دوره 14، شماره 1، آذر 1400، صفحه 81-91   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22034/ascij.2021.684778

نویسندگان

امین زاهدی انارکی¹؛ رامبد خواجه ای^{* 1}؛ امیر رشید لمیر²؛ امنه برجسته یزدی¹

¹گروه تربیت ‌بدنی و علوم ورزشی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

²گروه بیوشیمی و متابولیسم ورزشی، دانشکده علوم ورزشی, دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

گیرنده­های فعال­کننده­ی پرولیفراسیون پروگسیزوم گاما فاکتوری یک عامل رونویسی فعال‌شده به­وسیله لیگاند می­باشد که نقش مهمی در متابولیسم لیپیدها دارد. هدف از پژوهش حاضر اثر هشت هفته تمرینات هوازی-­مقاومتی بر بیان ژن گیرنده­­ی فعال­کننده­ پرولیفراسیون پروگسیزوم گاما در بیماران بای­پس عروق گرونری  بود. روش پژوهش­حاضرنیمه­تجربی و با طرح پیش­آزمون-پس­آزمون بود. جامعه آماری­ کلیه بیماران بای­پس عروق­ کرونری شهر مشهد بودند.در مطالعه حاضر نمونه پژوهشی شامل 26 مرد میان‌سال  با دامنه سنی 75/3 ± 73/56 (سال)، قد 03/0 ± 58/1 (متر)، وزن 54/5 ± 11/68 (کیلوگرم)،  شاخص توده بدن 47/2 ± 18/27 (کیلوگرم/مترمربع) و سابقه­ی بیماری 75/8 ± 20/25 (ماه) پس از عمل بای­پس عروق کرونر تشکیل‌شده بود. بر اساس معیارهای ورود به تحقیق، در دو گروه در تجربی (14) و کنترل (12) قرار گرفتند. داده­ها با استفاده از آزمون تی­مستقل وتی­همبسته در ­سطح معنی­داری 05/0≥p  و نرم­افزار SPSS نسخه 21تحلیل شدند. نتایج نشان داد که هشت هفته تمرینات هوازی-مقاومتی سبب افزایش بیان ژن PPAR در گروه تمرین ترکیبی نسبت به گروه کنترل شد (001/0=p ). همچنین نتایج آماری آزمون تی­همبسته نشان داد که افزایش بیان ژن PPAR در گروه تمرین ترکیبی افزایش معناداری در پس‌آزمون نسبت به پیش­آزمون داشت (001/0=p ) که این افزایش در گروه کنترل معنی­دار نبود (081/0=p ). داده­های پژوهش حاضر نشان داد تمرینات هوازی-مقاومتی با افزایش بیان ژن PPAR می­تواند در متابولیسم چربی­ها و فرآیند­های وابسته به آن در بیماران بای­پس عروق کرونری تأثیرگذار باشد.

کلیدواژه‌ها

تمرینات هوازی؛ تمرینات مقاومتی؛ PPAR؛ CABG

مراجع

1. Alavizadeh N., Rashidlamir A., Hejazi S.M. 2019. Effects of Eight Weeks of Cardiac Rehabilitation Training on Serum Levels of Sirtuin1 and Functional Capacity of Post-Coronary Artery Bypass Grafting Patients. Medical Laboratory Journal.;13(2): 41-47.
2. Berger J., Moller D.E. 2002. The mechanisms of action of PPARs. Annual review of medicine.53(1): 409-435.
3. Cabrero A., Laguna J., Vazquez M. 2002. Peroxisome proliferator-activated receptors and the control of inflammation. Current Drug Targets-Inflammation and Allergy.1(3): 243-248.
4. Culman J., Zhao Y., Gohlke P., Herdegen T. 2007. PPAR-γ: therapeutic target for ischemic stroke. Trends in Pharmacological Sciences, 28(5): 244-249.
5. Dallazen-Sartori F., Albuquerque L.C., Guaragna J., Magedanz E.H., Petracco J.B., Bodanese R. 2021. Risk Score for Prolonged Mechanical Ventilation in Coronary Artery Bypass Grafting. International Journal of Cardiovascular Sciences.34(3): 305-365.
6. Almeida F., Gambassi B.B., Schwingel P.A., Sauaia B.A., Sousa T.M., 2017.Possible benefits of different physical exercise programs after coronary artery bypass graft surgery: a minireview of selected randomized controlled trials. Sport Sciences for Health.13(3): 477-483.
7. Deswal A., Petersen N.J., Feldman A.M., Young J.B., White B.G., Mann D.L. 2001. Cytokines and cytokine receptors in advanced heart failure: an analysis of the cytokine database from the Vesnarinone trial (VEST). Circulation.103(16): 2055-2059.
8. Elibol B., Kilic U. 2018. High levels of SIRT1 expression as a protective mechanism against disease-related conditions. Frontiers in Endocrinology, 9: 614.
9. Parhizi f., RashidLamir A., Khajei R., Ramezanpour M., Vazifehdoost V. 2020. The effect of eight weeks of aerobic-resistance training on LXR gene expression and serum TNFα levels in CABG patients. Animal Biology, 12(2): 23-33. [in persian]
10. Fatone C., Guescini M., Balducci S., Battistoni S, Settequattrini A., Pippi R. 2010. Two weekly sessions of combined aerobic and resistance exercise are sufficient to provide beneficial effects in subjects with Type 2 diabetes mellitus and metabolic syndrome. Journal of endocrinological investigation, 33(7): 489-495.
11. Gullestad L., Ueland T., Vinge L.E., Finsen A., Yndestad A., Aukrust P. 2012. Inflammatory cytokines in heart failure: mediators and markers. Cardiology, 122(1): 23-35.
12. Hamidi A., Rashidlamir A., Khajei R., Zarei M., Zendedel A. 2020. The effect of aerobic-resistance training on plasma levels of bFGF in .coronary artery disease after CABG. Journal of Arak University of Medical Sciences, 23(3): 314-325. [in persian]
13. Holm L.J., Mønsted M.Q., Haupt-Jorgensen M., Buschard K. 2020. PPARs and the Development of Type 1 Diabetes. PPAR Research, 2020(10): 40-51.
14. Honda T., Kaikita K., Tsujita K., Hayasaki T., Matsukawa M., Fuchigami S. 2008. Pioglitazone, a peroxisome proliferator-activated receptor-γ agonist, attenuates myocardial ischemia–reperfusion injury in mice with metabolic disorders. Journal of Molecular and Cellular Cardiology, 44(5): 915-926.
15. Jeremic N., Chaturvedi P., Tyagi S.C. 2017. Browning of white fat: novel insight into factors, mechanisms, and therapeutics. Journal of Cellular Physiology, 232(1): 61-68.
16. Kajimura S., Spiegelman B.M., Seale P. 2015. Brown and beige fat: physiological roles beyond heat generation. Cell metabolism, 22(4): 546-559.
17. Kamimura D., Uchino K., Ishigami T., Hall M.E., Umemura S. 2016. Activation of peroxisome proliferator-activated receptor γ prevents development of heart failure with preserved ejection fraction; inhibition of Wnt-β-catenin signaling as a possible mechanism. Journal of Cardiovascular Pharmacology, 68(2):155-161.
18. Kersten S., Desvergne B., Wahli W., 2000. Roles of PPARs in health and disease. Nature, 405(6785): 421-424.
19. Kloc M., Uosef A., Kubiak J.Z., Ghobrial R.M. 2021. Role of Macrophages and RhoA Pathway in Atherosclerosis. International Journal of Molecular Sciences, 22(1): 216.
20. Li M., Bai Y., Jianfei C., Xiaodong X., Yuanyuan D., Jing Z. 2014. Effects of different exercise intensity on PPARγ and relative index in adolescent obesity rats. Wei sheng yan jiu Journal of Hygiene Research, 43(5): 732-737.
21. Liu W-X., Wang T., Zhou F., Wang Y., Xing J-W., Zhang S. 2015. Voluntary exercise prevents colonic inflammation in high-fat diet-induced obese mice by up-regulating PPAR-γ activity. Biochemical and Biophysical Research Communications, 459(3): 475-480.
22. Liu Z., Ding J., McMillen T.S., Villet O., Tian R., Shao D. 2020. Enhancing fatty acid oxidation negatively regulates PPARs signaling in the heart. Journal of Molecular and Cellular Cardiology, 146: 1-11.
23. Lo K.A., Sun L. 2013. Turning WAT into BAT: a review on regulators controlling the browning of white adipocytes. Bioscience Reports, 33(5): e00065.
24. Lygate C.A., Hulbert K., Monfared M., Cole M.A., Clarke K., Neubauer S. 2003. The PPARγ-activator rosiglitazone does not alter remodeling but increases mortality in rats post-myocardial infarction. Cardiovascular Research, 58(3): 632-637.
25. Naghibzade A, Rashidlamir A, Khajeie R, Zarei M, Safipour A.A. 2019. The Effect of combined rehabilitation training on plasma levels and ghrelin gene expression in PBMNC among CABG patients.,12(1): 69-80.
26. Okamoto T., Masuhara M., Ikuta K. 2007. Combined aerobic and resistance training and vascular function: effect of aerobic exercise before and after resistance training. Journal of Applied Physiology, 103(5): 1655-16561.
27. Petridou A., Tsalouhidou S., Tsalis G., Schulz T., Michna H., Mougios V. 2007. Long-term exercise increases the DNA binding activity of peroxisome proliferator–activated receptor γ in rat adipose tissue. Metabolism, 56(8): 1029-1036.
28. Rashidlamir A., Dastani M., Saadatnia A., Bassami M.R. 2018. Effect of Cardiac Rehabilitation Training on ABCA1 Expression in Lymphocytes of Patients Undergoing Coronary Artery Bypass Graft Operation. Zahedan Journal of Research in Medical Sciences, 20(6): e11277. [in persian]
29. Razaghi A., Sadeghi H., Johari Moghadam A., Motamedi P. 2020. The effect of exercise-based cardiac rehabilitation in two ways aerobic and aerobic-resistance exercises on the biomechanical function of cardiac patients (MI, PCI, and CABG). Razi Journal of Medical Sciences, 26(12): 138-148. [in persian]
30. Sahhaf F., Nazari M., Dorosti A. 2020. Effect of eight weeks intermittent exercise training on oxidative stress indices in women with preterm labor after coronary artery bypass graft surgery (CABG): randomized clinical trial. The Iranian Journal of Obstetrics, Gynecology and Infertility, 23(3): 1-8. [in persian]
31. Sarhangi N., Sharifi F., Hashemian L., Doabsari MH., Heshmatzad K., Rahbaran M. 2020. PPARG (Pro12Ala) genetic variant and risk of T2DM: a systematic review and meta-analysis. Scientific Reports, 10(1): 1-18.
32. Seiri P., Abi A., Soukhtanloo M., 2019. PPAR‐γ: Its ligand and its regulation by microRNAs. Journal of Cellular Biochemistry, 120(7): 10893-10908.
33. Shabani M., Daryanoosh F., Salesi M., Kooshki Jahromi M., Fallahi A.A., 2018. Effect of continuous training on the level of PPAR-γ and PRDM16 proteins in adipose tissue in overweight diabetes rats. The Journal of Qazvin University of Medical Sciences, 22(3): 4-12. [in persian]
34. Shoaib A., Mohamed M., Rashid M., Khan S.U., Parwani P., Contractor T. 2021. Clinical characteristics, management strategies and outcomes of acute myocardial infarction patients with prior coronary artery bypass grafting. Mayo Clinic Proceedings: Elsevier, 96(1): 120-131.
35. Sidi S., Khajei R., RashidLamir A., Ramazanpour M. 2020. The effect of cardiac rehabilitation on endostatin levels in patients with atherosclerosis after coronary artery bypass grafting. Journal of Arak University of Medical Sciences, 23(4): 1-11. [in persian]
36. Silveira L.S., Biondo L.A., Teixeira A.A., Junior E.A., Castoldi A., Câmara N.O.S. 2020. Macrophage immunophenotype but not anti-inflammatory profile is modulated by peroxisome proliferator-activated receptor gamma (PPARgamma) in exercised obese mice. Exercise and Immunology Review, 26: 10-22.
37. Srivastava R.A.K. 2011. Evaluation of anti-atherosclerotic activities of PPAR-α, PPAR-γ, and LXR agonists in hyperlipidemic atherosclerosis-susceptible F1B hamsters. Atherosclerosis, 214(1): 86-93.
38. Suskin N., Faubert C., McKelvie R., 2021. Post Cardiac Surgery Rehabilitation. Evidence-Based Practice in Perioperative Cardiac Anesthesia and Surgery: Springer, pp. 687-696.
39. Wagner N., Wagner K.D. 2020. PPARs and angiogenesis—Implications in pathology. International Journal of Molecular Sciences, 21(16): 5723.
40. Wątroba M., Szukiewicz D. 2016. The role of sirtuins in aging and age-related diseases. Advances in Medical Sciences, 61(1): 52-62.
41. Wojtkowska I., Bonda T.A., Wolszakiewicz J., Osak J., Tysarowski A., Seliga K. 2017. Myocardial Expression of PPARγ and Exercise Capacity in Patients after Coronary Artery Bypass Surgery. PPAR Research, 2017: 7
42. Xu H., Barnes G.T., Yang Q., Tan G., Yang D., Chou C.J. 2003. Chronic inflammation in fat plays a crucial role in the development of obesity-related insulin resistance. The Journal of Clinical Investigation, 112(12): 1821-1830.
43. Xu Y., Gen M., Lu L., Fox J., Weiss S.O., Brown R.D. 2005. PPAR-γ activation fails to provide myocardial protection in ischemia and reperfusion in pigs. American Journal of Physiology-Heart and Circulatory Physiology, 288(3): H1314-H1323.
44. Ye Y., Hu Z., Lin Y., Zhang C., Perez-Polo J.R. 2010. Downregulation of microRNA-29 by antisense inhibitors and a PPAR-γ agonist protects against myocardial ischaemia–reperfusioninjury. Cardiovascul ar Research, 87(3): 535-544.
45. Zhang Y., Yang X., Bian F., Wu P., Xing S., Xu G. 2014. TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ. Journal of Molecular and Cellular Cardiology, 72: 85-94.