HPLC profile, antioxidant, antibacterial, and anti-proliferative activity of aerial parts of Ferula ovina (Boiss.) Boiss.

Trends in Phytochemical Research

دوره 5، شماره 3، آذر 2021، صفحه 148-154   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/tpr.2021.1929063.1207

نویسندگان

Mohamad Hassan Mohamadi^{* 1}؛ Behnam Mahdavi²؛ Esmaeil Rezaei-Seresht²؛ Sahar Riahi-Farsani²؛ Nazanin Entezari³

¹Department of Chemistry, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran

²Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran

³Department of Anesthesiology, Faculty of Paramedical, Sabzevar University of Medical Sciences, Sabzevar, Iran

چکیده

The aerial parts of Ferula ovina (Boiss.) Boiss.  were extracted by methanol and then fractionated with solvents with different polarity. The evaluation of the bioactivity of the plant extracts including antioxidant, antimicrobial and antiproliferative activity was carried out using various assays. The phenolic and flavonoid compound profile of the extract were established using HPLC analysis. The leaves showed the highest total phenolic content and radical scavenging activity; the flowers had the maximum amount of total flavonoid content, and the stems exhibited the maximum ferrous ion chelating ability. Among ten microorganisms, the plant extract prevented the growth of three strains, namely Pseudomonas aeruginosa, Acinetobacter baumannii, and Streptococcus pneumonia. The flower of F. ovina (Boiss.) Boiss shows the best anti-human cervical carcinoma property against HeLa cell lines. Gallic acid was found as the major phenolic compound in the plant extract according to the HPLC analysis.

کلیدواژه‌ها

Antibacterial activity؛ Antioxidant activity؛ Ferrous ion chelating؛ Ferula ovina؛ Radical scavenging activity

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