Immunomodulatory Effect of Propolis on IFN-γ, IL-17A and IL-23 Production in Human Peripheral Blood Mononuclear Cells Treated with Pseudomonas aeruginosa Ag

Journal of Chemical Health Risks

دوره 11، Special Issue: Bioactive Compounds: Their Role in the Prevention and Treatment of Diseases، دی 2021، صفحه 143-151   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.22034/jchr.2021.685425

نویسندگان

Farah Alaa Alwaeely^{* 1}؛ Mohammad Abd kadhum Alsaadi²؛ Kaiser N. Madlum²

¹Department of Microbiology, Faculty of Medicine, Babylon University, Najaf, Iraq

²Department of Microbiology, Faculty of Medicine, Babylon, University Hilla, Iraq

چکیده

Propolis has many potential effects on the immune response against P. aeruginosa both cellular and humoral immunity. Aim of this study is to verify the anti-Pseudomonas property of ethanol and water extracted propolis on PBMCs stimulated with P. aeruginosa Ag. In this study a total of (20) apparently healthy volunteers consist of (10) males and (10) females were included, their ages ranged from (20-40) years. Five mL of blood were taken from each included subject for evaluation of IFN-γ, IL-17A and IL-23 in peripheral blood mononuclear cells (PBMCs) that were isolated from blood using density gradient lymphoprep and stimulated with pseudomonas aeruginosa LPS in to four groups, LPS stimulated PBMCs, Ethanol extracted propolis EEP+LPS stimulated PBMCs, water extracted propolis WEP+LPS stimulated PBMCs and control (PBMCs) by ELISA technique after 48Hrs. The results showed that both the EEP and WEP could significantly inhibit pro-inflammatory cytokines production by human PBMC after stimulation with pseudomonas Ag. Propolis exhibits an anti-pseudomonas aeruginosa with the same effect with ethanol and water extracts.

کلیدواژه‌ها

Antibiotic resistance؛ Pseudomonas aeruginosa immunomodulatory؛ Propolis؛ IFN-γ؛ IL-17A؛ IL-23

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