Investigation the effect of [Met-Hcl] [Pys] ionic liquid (ILA) on candida albicans standard strain and evaluation of its toxicity on mammalian cells

International Journal of Molecular and Clinical Microbiology

دوره 11، شماره 2، اسفند 2021، صفحه 1509-1514   

نوع مقاله: Research Article

نویسندگان

Mona Eslami¹؛ Ali Ezabadi^{* 2}؛ Mansour Bayat³؛ Behin Omidi⁴؛ Pejman Mortazavi⁵

¹1. Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

²Department of Chemistry, Faculty of Science, Islamic Azad University, Central Tehran Branch, Iran.

³Department of Pathobiology, Faculty of Veterinary Specialized Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

⁴Department of Biology, Islamic Azad University, Central Tehran Branch, Tehran, Iran

⁵Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

چکیده

Recently, fungal drug resistance has significantly increased especially in opportunistic fungus like Candida albicans. Accordingly, it is necessary to use more effective drugs with less toxicity and high influences. Among a large of investigations, ionic liquids showed biological influence and antimicrobial activities. The aim of this study was in vitro investigation of antifungal activity of pyridine –based ionic liquid on a standard strain of Candida albicans and evaluate its toxicity on host cells. A standard strain of Candida albicans was re- cultured on Sabouraud Dextrose Agar (SDA) containing chloramphenicol, incubated at 37℃ for 24 h. Antifungal effect of a novel ionic liquid ([Met-Hcl] [Pys] ), was evaluated using inhibitory zone diameter. The minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) were performed using micro dilution method. In continue MTT test were done to evaluate the toxicity of the liquid on host cells. The ionic liquid showed a good effect to prevent of Candida albicans growth. Inhibitory zone diameter was between 34±1 mm. The MIC evaluation was 708.4 ppm. Also the results of MTT test showed the viability of host cells at the 16 dilution.
In conclusion, the results of this study manifested that the novel ionic liquid has a good antifungal activity against Candida albicans standard strain with low toxicity to human cells and probably can use as a good novel drug in treatment of candidiasis. Although the need for more studies is crystal clear.

کلیدواژه‌ها

candidiasis؛ ionic liquid؛ Cytotoxicity؛ antifungal؛ MTT