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Derivative- Ratio Spectrophotometric, Chemometric And Hplc Validated Methods For Simultaneous Determination Of Amlodipine And Atorvastatin In Combined Dosage Form | ||
International Journal of Industrial Chemistry | ||
دوره 2، شماره 2، شهریور 2011، صفحه 78-85 اصل مقاله (640.9 K) | ||
نوع مقاله: research article | ||
نویسندگان | ||
Ola Moustafa Abdallah* 1؛ Amr Mohamed Badawey2 | ||
1Analytical Chemistry Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 26 July Mehwer Road intersection with Wahat Road, 6th October City, Egypt | ||
2Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt | ||
چکیده | ||
Three methods were developed for simultaneous determination of amlodipine and atorvastatin without previous separation. The first method depends on first derivative of the ratios spectra by measurements of the amplitudes at 228 and 245 nm for amlodipine using 25 mg/mL of atorvastatin as a divisor and at 284 and 295 nm for atorvastatin using 80 mg/mL of amlodipine as a divisor. Calibration graphs were established in the range of 10-100 mg/mL and 2.5-30 mg/mL for amlodipine and atorvastatin, respectively. The second method describes the use of multivariate spectrophotometric calibration for the simultaneous determination of the analyzed binary mixture, where the resolution is accomplished by using partial least squares (PLS) regression analysis. In the third method (HPLC), separation was performed by using C18 reversed phase column and a mobile phase of acetonitrile: 0.05 M KH2PO4 (60:40v/v) adjusted by phosphoric acid to pH 3.5 at flow rate of 1 mL/min. All proposed methods were extensively validated and the results obtained by adopting the three methods were statistically analyzed and compared with those obtained from a reported method. | ||
کلیدواژهها | ||
AMLODIPINE؛ ATORVASTATIN؛ DERIVATIVE RATIO؛ PLS؛ HPLC | ||
آمار تعداد مشاهده مقاله: 29 تعداد دریافت فایل اصل مقاله: 24 |