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Aderibigbe, Segun Abidemi, Adegoke, Olajire Aremu, Idowu, Olakunle Sunday. (1391). A new colorimetric method for the determination of nifedipine tablets by derivatization using 4-carboxyl-2,6-dinitrobenzene diazonium ion. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 3(1), 208-215. doi: https://doi.org/10.1186/2228-5547-3-5
Segun Abidemi Aderibigbe; Olajire Aremu Adegoke; Olakunle Sunday Idowu. "A new colorimetric method for the determination of nifedipine tablets by derivatization using 4-carboxyl-2,6-dinitrobenzene diazonium ion". نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 3, 1, 1391, 208-215. doi: https://doi.org/10.1186/2228-5547-3-5
Aderibigbe, Segun Abidemi, Adegoke, Olajire Aremu, Idowu, Olakunle Sunday. (1391). 'A new colorimetric method for the determination of nifedipine tablets by derivatization using 4-carboxyl-2,6-dinitrobenzene diazonium ion', نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 3(1), pp. 208-215. doi: https://doi.org/10.1186/2228-5547-3-5
Aderibigbe, Segun Abidemi, Adegoke, Olajire Aremu, Idowu, Olakunle Sunday. A new colorimetric method for the determination of nifedipine tablets by derivatization using 4-carboxyl-2,6-dinitrobenzene diazonium ion. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 1391; 3(1): 208-215. doi: https://doi.org/10.1186/2228-5547-3-5

A new colorimetric method for the determination of nifedipine tablets by derivatization using 4-carboxyl-2,6-dinitrobenzene diazonium ion

International Journal of Industrial Chemistry
دوره 3، شماره 1، اسفند 2012، صفحه 208-215
نوع مقاله: research article
شناسه دیجیتال (DOI): https://doi.org/10.1186/2228-5547-3-5
نویسندگان
Segun Abidemi Aderibigbe؛ Olajire Aremu Adegoke* ؛ Olakunle Sunday Idowu
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan, Orita UI, Oyo Road, Ibadan, 200001, Nigeria
چکیده
Background
A new sensitive colorimetric determination of nifedipine has been developed following azo dye formation with 4-carboxyl-2,6-dinitrobenzenediazonium ion (CDNBD). Judging from the various generic brands currently now available, this research was conceived as a means of developing an alternative cost-effective and readily adaptable method for the assay of nifedipine in tablets and for which official high performance liquid chromatographic technique may not be readily available.
Results
Nifedipine was reduced with Zn/HCl reduction system and then the diazo coupling reaction was carried out with the CDNBD reagent to generate a new azo adduct with optimal wavelength at 470 nm representing a bathochromic shift relative to nifedipine, reduced nifedipine and CDNBD reagent. Optimal temperature and time for coupling were selected as 50 oC and 15 minutes. A linear response was observed over 2.9 -14.5 µg/mL of nifedipine with a correlation coefficient of 0.9985 and the drug combined with CDNBD at a stoichiometric ratio of 2:1. The method has limits of detection and quantitation of 0.1344 µg mL-1 and 0.4074 µg mL-1 respectively. The Sandell’s sensitivity obtained is 4.673 ng/cm2 and the method was reproducible over a three day assessment. Intra- and inter-day accuracies (in terms of errors) were of the order -0.008 to 3.22 % while precisions were generally less than 3.1 % (RSD).
Conclusions
The developed spectrophotometric method is of equivalent accuracy (p > 0.05) with USP 2007 HPLC method. It has the advantages of speed, simplicity, sensitivity and more affordable instrumentation and could find application as a rapid and sensitive analytical method for nifedipine. It is the first described method by azo dye derivatization for the analysis of nifedipine in bulk samples and dosage forms.
کلیدواژه‌ها
Nifedipine tablets؛ 4-carboxyl-2؛ 6-dinitrobenzene diazonium ion؛ Azo dye derivatization؛ Colorimetric analysis
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تعداد مشاهده مقاله: 33


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