Sensitive LC method for the simultaneous determination of diacerein and aceclofenac in tablet dosage form

International Journal of Industrial Chemistry

دوره 3، شماره 1، اسفند 2012، صفحه 220-226

نوع مقاله: research article

شناسه دیجیتال (DOI): https://doi.org/10.1186/2228-5547-3-3

نویسندگان

Shaikh kabeer Ahmed^{* 1}؛ Ashish Tanaji Patil²؛ Audumber Bhagwan Ingole²

¹P. G. Department of Chemistry, Sir Sayyed College, P.B. No. 89, Roshan gate, Aurangabad, M.S., 431001, India.

²Post Graduate Department of Chemistry, Sir Sayyed College, 89 Roshan Gate, Aurangabad, 431001, MS, India

چکیده

Background
Diacerein and aceclofenac drug product combination is used for the pain management in osteoarthritis. The main aspects of the present study is to develop stability indicating, sensitive reverse phase high-performance liquid chromatographic method for the simultaneous determination of diacerein and ceclofenac in tablet dosage form. Were, the chromatographic separation was achieved on Kromasil C-18, 150 × 4.6 mm, 3.5-μm analytical column using mobile phase double distilled water (pH 2.7 with glacial acetic acid)-acetonitrile (45:55 v/v). Detector was set at 256 nm.
Results
The described method shows excellent linearity over a range of 75–2.5 μg/mL for diacerein and 150–5 μg/mL for aceclofenac. The correlation coefficient for diacerein is 0.9999 and for aceclofenac is 0.9997.
Conclusions
The proposed method was found to be suitable for simultaneous determination and stability study of diacerein and aceclofenac in tablet dosage form.

کلیدواژه‌ها

Aceclofenac؛ Diacerein؛ Forced degradation؛ Method validation؛ RP-HPLC