Phytochemical constituents found in Physalis peruviana L. leaf extracts and their ability to inhibit alpha-glucosidase and scavenge DPPH free radicals in vitro

Trends in Phytochemical Research

دوره 6، شماره 1، مرداد 2022، صفحه 3-10   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/tpr.2022.1946170.1230

نویسندگان

Félicien Mushagalusa Kasali^{* 1، 2، 3}؛ Muhammad Shaiq Ali⁴؛ Jonans Tusiimire²؛ Mehreen Lateef⁵؛ Ruth Anyango Omole⁶؛ Justin Ntokamunda Kadima^{3، 7}؛ Amon Ganafa Agaba⁸

¹Pharm-Bio Technology and Traditional Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda

²Department of Pharmacy, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda

³Department of Pharmacy, Faculty of Pharmaceutical Sciences and Public Health, Official University of Bukavu, PO Box 570 Bukavu, the Democratic Republic of Congo

⁴HEJ Research Institute of Chemistry, International Centre for Chemical & Biological Sciences, University of Karachi, Karachi-75270, Pakistan

⁵Multi-Disciplinary Research Lab, Bahria University, Medical & Dental College, Karachi, Pakistan

⁶School of Chemistry and Material Science, Technical University of Kenya, P.O Box 52428- 00200, Nairobi, Kenya

⁷Department of Pharmacology, School of Medicine and Pharmacy, University of Rwanda, PO Box 117 Huye, Rwanda Mbarara

⁸Department of Pharmacology and Therapeutics, Faculty of Medicine, Mbarara University of Science and Technology, PO Box 1410, Mbarara, Uganda

چکیده

Physalis peruviana L. is widely used in folk medicines to manage diabetes. This study aimed to identify new chemical constituents and explore in vitro antioxidant ability and alpha-glucosidase inhibition activity to explain its antidiabetic mechanism. Methanolic extracts and hexane fractions were prepared and analyzed by CG-FID and GC-MS. In vitro antidiabetic activity was assessed on α-glucosidase inhibition and DPPH models. The phytochemical analysis detected 29 constituents, manly heptacosane (25) and hexadecanoic acid methyl ester (14). Aliphatic hydrocarbons and esters were identified as the main classes of secondary metabolites. Compared to the standard, the methanol-water fraction was the most potent fraction on α-glucosidase inhibition (IC50 32.5 ± 0.27 µg/mL). The antioxidant values (IC50) of two fractions, and the reference were quite close (44.5 ± 0.54; 49.5 ± 0.87; 44.2 ± 0.77 µg/mL). Almost 82.75% are recognized for the first time in the leaf hexane fractions of this plant. The observed antioxidant activity was found to be significantly notable.

کلیدواژه‌ها

Bio-compounds؛ GC-FID؛ Hexane fraction؛ MS؛ Physalis peruviana L

مراجع

Bailly, C., 2021. Bioactive biflavonoids from Wikstroemia indica (L.) C.A. Mey. (Thymelaeaceae): a review. Trends Phytochem. Res. 5(4), 190-198.
Ballesteros-Vivas, D., Álvarez-Rivera, G., Ibáñez, E., Parada-Alfonso, F., Cifuentes, A., 2019. A multi-analytical platform based on pressurized-liquid extraction, in vitro assays and liquid chromatography/gas chromatography coupled to high resolution mass spectrometry for food by-products valorisation. Part 2: Characterization of bioactive compound. J. Chromatogr. A 1584, 155-164.
Bandaranayake, W.M., 2006. Quality control, screening, toxicity, and regulation of herbal drugs, In: Ahmad, I., Aqil, F., Owais, M. (Eds.), Modern Phytomedicine. Turning Medicinal Plants into Drugs. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, pp. 25-57.
Bongmo, V.L.L., Happi, G.M., Tabekoueng, B., Lateef, M., François, A., Waffo, K., Ali, M.S., Choudhary, M.I., Nouga, B., Wansi, J.D., 2022. Phytochemical compounds of Guibourtia ehie and their antioxidant, urease and α-glucosidase inhibitory activities. Nat. Resour. Hum. Heal. 1-7.
Dymerski, T., Namieśnik, J., Leontowicz, H., Leontowicz, M., Vearasilp, K., Martinez-Ayala, A.L., González-Aguilar, G.A., Robles-Sánchez, M., Gorinstein, S., 2016. Chemistry and biological properties of berry volatiles by two-dimensional chromatography, fluorescence and Fourier transform infrared spectroscopy techniques. Food Res. Int. 83, 74-86.
Eken, A., Ünlü-Endirlik, B., Baldemir, A., İlgün, S., Soykut, B., Erdem, O., Akay, C., 2016. Antioxidant capacity and metal content of Physalis peruviana L. fruit sold in markets. J. Clin. Anal. Med. 7, 291-294.
Gülçin, I., Alici, H.A., Cesur, M., 2005. Determination of in vitro antioxidant and radical scavenging activities of propofol. Chem. Pharm. Bull. 53, 281-285.
Indrayanto, G., Putra, G.S., Suhud, F., 2021. Validation of in-vitro bioassay methods: Application in herbal drug research, in: Profiles of drug substances, excipients and related methodology. Elsevier Inc., pp. 273-307.
Kasali, M.F., Mahano, A.O., Bwironde, F.M., Amani, A.C., Mangambu, J.D., Nyakabwa, D.S.,
Wimba, L.K., Tshibangu, D.S.T., Ngbolua, K.N., Kambale, J.K., Mpiana, P.T., 2013.
Ethnopharmacological survey of plants used against diabetes in Bukavu city (D.R. Congo). 
J. Ethnobiol. Tradit. Med. 119, 538–546.
Kasali, F.M., Kadima, J.N., Peter, E.L., Mtewa, A.G., Ajayi, C.O., Tusiimire, J., Tolo, C.U., 
Ogwang, P.E., Weisheit, A., Agaba, A.G., 2021. Antidiabetic medicinal plants used in
Democratic Republic of Congo : A critical review of ethnopharmacology and bioactivity 
data. Front. Pharmacol. 12, 7579090. DOI: org/10.3389/fphar.2021.757090.
Kasali, F.M., Tuyiringire, N., Peter, E.L., Ahovegbe, L.Y., Ali, M.S., Tusiimire, J., Ogwang, P.E., Kadima, N.J., Agaba, A.G., 2022. Chemical constituents and evidence-based pharmacological properties of Physalis peruviana L .: An overview. J. Herbmed Pharmacol. 1, 35-47.
Kemayou, G.P.M., Kache, S.F., Dzouemo, L.C., Happi, G.M., Kouam, S.F., Tchouankeu, J.C., 
2021. Phytochemistry, traditional uses, and pharmacology of the genus Ekebergia
(Meliaceae): A review. Trends Phytochem. Res. 5, 110–125. 
Khan, K., Firdous, S., Ahmad, A., Fayyaz, N., Nadir, M., Rasheed, M., Faizi, S., 2016. GC-MS profile of antimicrobial and antioxidant fractions from Cordia rothii roots. Pharm. Biol. 54, 2597-2605. 
Kumar, S., Pandey, A.K., 2013. Chemistry and biological activities of flavonoids: An overview. Sci. World J. 2013, DOI: org/10.1155/2013/162750.
Kurihara, H., Sasaki, M., Hatano, M., 1994. A new screening method for glucosidase inhibitors and its application to algal extracts. Fish. Sci. 60, 759-761.
Lin, D., Xiao, M., Zhao, J., Li, Z., Xing, B., Li, X., Kong, M., Li, L., Zhang, Q., Liu, Y., Chen, H., Qin, W., Wu, H., Chen, S., 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. Molecules 21, DOI: org/10.3390/molecules21101374.
Majcher, M.A., Scheibe, M., Jeleń, H.H., 2020. Identification of odor active compounds in Physalis peruviana L. Molecules 25, DOI: org/10.3390/molecules25020245.
Mayorga, H., Knapp, H., Winterhalter, P., Duque, C., 2001. Glycosidically bound flavor compounds of cape gooseberry (Physalis peruviana L.). J. Agric. Food Chem. 49, 1904-1908.
Medina-Pérez, G., Zaldívar-Ortega, A.K., Cenobio-Galindo, A. de J., Afanador-Barajas, L.N., Vieyra-Alberto, R., Estefes-Duarte, J.A., Campos-Montiel, R.G., 2019. Antidiabetic activity of cactus acid fruit extracts: Simulated intestinal conditions of the inhibitory effects on α-amylase and α-glucosidase. Appl. Sci. 9, DOI: org/10.3390/app9194066.
Mohammadhosseini, M., Frezza, C., Venditti, A., Sarker, S.D., 2021a. A systematic review 
on phytochemistry, ethnobotany and biological activities of the genus Bunium L. Chem. 
Biodivers. 18, e2100317. DOI: org/10.1002/cbdv.202100317.
Mohammadhosseini, M., Venditti, A., Frezza, C., Serafini, M., Bianco, A., Mahdavi, B., 
2021b. The genus Haplophyllum Juss.: Phytochemistry and bioactivities—A review. 
Molecules 26, 4664. DOI: org/10.3390/molecules26154664.
Mohammed, Z.H., Ibraheem, R.M., 2015. Antioxidant activity of methanol extracts of Arum maculatum L. and Physalis peruviana L. plants. Ibn Al-Haitham J. Pure Appl. Sci. 28, 1-7.
Peter, K.K., Zipporah, N., Francis, M.N., John, T., 2020. In vitro antiplasmodial, cytotoxicity assay and partial chemical characterization of Kenyan Physalis peruviana L. (Solanaceae family) extracts. J. Med. Plants Res. 14, 73-80.
Pillai, G.G.K., Bharate, S.S., Awasthi, A., Verma, R., Mishra, G., Singh, A.T., Jaggi, M., Mithal, A., Vishwakarma, R.A., 2017. Antidiabetic potential of polyherbal formulation DB14201: Preclinical development, safety and efficacy studies. J. Ethnopharmacol. 197, 218-230.
Pinto, M.D.S., Ranilla, L.G., Apostolidis, E., Lajolo, F.M., Genovese, M.I., Shetty, K., 2009. Evaluation of antihyperglycemia and antihypertension potential of native Peruvian fruits using In vitro models. J. Med. Food 12, 278-291.
Ramadan, M.M., El-Ghorab, A.H., Ghanem, K.Z., 2015. Volatile compounds, antioxidants, and anticancer activities of Cape gooseberry fruit (Physalis peruviana L.): an in-vitro study. J. Arab Soc. Med. Res. 10, 56-64.
Ramadan, M.F., Möersel, J.T., 2003. Oil goldenberry (Physalis peruviana L.). J. Agric. Food Chem. 51, 969-974.
Ramadan, M.F., Möersel, J.T., 2007. Impact of enzymatic treatment on chemical composition, physicochemical properties and radical scavenging activity of goldenberry (Physalis peruviana L.) juice. J. Sci. Food Agric. 87, 452-460.
Rodrigues, E., Rockenbach, I.I., Cataneo, C., Gonzaga, L.V., Chaves, E.S., Fett, R., 2009. Minerals and essential fatty acids of the exotic fruit Physalis peruviana L. Ciência e Tecnol. Aliment. 29, 642-645.
Sathasivampillai, S. V., Rajamanoharan, P.R.S., Munday, M., Heinrich, M., 2017. Plants used to treat diabetes in Sri Lankan Siddha Medicine - An ethnopharmacological review of historical and modern sources. J. Ethnopharmacol. 198, 531-599.
Susmita, D., Sukriti, D., Bratati, D., 2012. In vitro inhibition of key enzymes related to diabetes by the aqueous extracts of some fruits of West Bengal, India. Curr. Nutr. Food Sci. 8, 19-24.
Vega-Gálvez, A., López, J., Torres-Ossandón, M.J., Galotto, M.J., Puente-Díaz, L., Quispe-Fuentes, I., Di Scala, K., 2014. High hydrostatic pressure effect on chemical composition, color, phenolic acids and antioxidant capacity of Cape gooseberry pulp (Physalis peruviana L.). LWT - Food Sci. Technol. 58, 519-526.
Venditti, A., 2018. What is and what should never be: artifacts, improbable phytochemicals, contaminants and natural products. Nat. Prod. Res. 34, 1014-1031.
Yilmaztekin, M., 2014. Analysis of volatile components of cape gooseberry (Physalis peruviana L.) grown in turkey by HS-SPME and GC-MS. Sci. World J. 2014, DOI: org/10.1155/2014/796097.