Effects of Plant Growth Regulators, Culture Media, and Light on Bulblet Production of Lily ʻFujianʼ Using Scaling Technique

Journal of Ornamental Plants

مقاله 1، دوره 12، شماره 1، خرداد 2022، صفحه 1-10   

نوع مقاله: Research Paper

نویسندگان

Abolfazl Aliverdi¹؛ Davoud Asgari^{* 2}

¹M.Sc. Student Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

²Assistant professor Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

چکیده

Lily (Lilium spp.) is one of the most important flower in ornamental plants industry. This flower is one of the geophyte plants, therefore lilies cultivation was done with bulb. Between different lily bulb producing methods, scaling is a cost-effective and short-term technique. Lily bulb producers have always various challenges in different stage of production. Aiming to focus on solving these challenges, two separate experiments were performed to optimize bulblet production, as the first stage of bulb production. In the first experiment, four concentrations of two types of auxin (indole-3- butyric acid and naphthalene acetic acid at concentrations of 250 and 500 mg/L) and a control treatment as the first factor and effects of three culture media, including coco peat, coco peat + perlite (1:1) and black peat moss, as the second factor were examined. Results indicated that although the highest number of bulblets per scale (2.07) was observed in concentration of 500 mg/L of indole-3- butyric acid, but the maximum bulblet’s weight (0.81 g) and bulblet’s diameter (1.32 cm) were produced under control treatment. Regarding culture media effects, the highest number of bulblet per scale (2.02) maximum bulblet’s weight (0.71 g) and bulblets’ diameter (1.21 cm) were obtained in peat moss media. In the second experiment, above mentioned concentrations of the plant growth regulators as a first factor and also the presence or absence of light as a second factor was examined. Results showed that present or absent of light has not significant effects on all evaluated traits except bulblet’s diameter. Bulblet diameter significantly increased in dark situation. Extract of this study indicated, in lily bulbs production, with using PGR treatment can improve some quantity and quality traits.

کلیدواژه‌ها

Geophyte؛ Indole-3-butyric acid؛ Naphthalene acetic acid؛ Peat moss

مراجع

Akcal, A. and Kahraman, Ö. 2016. Different approaches on bulblet formation with scaling in Madona lily (Lililium candidum). Scientific Papers-Series B, Horticulture, 60: 209-216.‏

Bakhshaie, M., Babalar, M., Amirmasoudi, M. and Khalighi, A. 2010. Somatic embryogenesis and plant regeneration of Lilium ledebourii Bioss. and endangered species. Journal of Plant Physiology, 102: 229- 253.

Bakhshaie, M., Khosravi, S., Azadi, P., Bagheri, H. and van Tuyl, J.M. 2016. Biotechnological advances in Lilium. Plant Cell Reports, 35 (9): 1799-1826.

Chung, H.J., Choi, C.H. and Park, N.B. 1995. Effects of temperature, culturing media and auxin treatment on bulblet formation in scaling of lilies. Journal of the Korean Society for Horticultural Science, 36 (4): 567-573.

Dantu, P.K. and Bhojwani, S.S. 1995. In vitro corm formation and field evaluation of corm-derived plants of gladiolu. Scientia Horticulturae, 61: 115-129.

Dhiman, M.R. and Sindhu, S.S. 2007. Effect of propagation media and growth regulators on bulblet formation through scale propagation in Lilium. Journal of Ornamental Horticulture, 10 (3): 181-183.‏

Dole, J.M. and Wilkins, H.F. 2005. Floriculture principles and species. 2nd edn. Person Education. Inc. New Jersey.

Gupta, Y. C., Dhiman, S. R. and Kashyap, B. 2010. Effect of growing media and auxins on propagation of hybrid lilies through scaling. In: National Symposium on Lifestyle Floriculture: Challenges and Opportunities, Nauni, Solan, Book of Abstracts. 50 p.

Gupta, Y.C., Dhiman, S.R., Kashyap, B. and Sharma, M. 2016. Effect of auxins and growing media on propagation of Lilium through scaling. Journal of Ornamental Horticulture, 19 (3 and 4): 111-118.

Hatamzadeh, A., Shafiee-Masouleh, S.S., Samizadeh, H. and Rad-Moghadam, K. 2015. Extending storage duration of mother scales for enlarging scale bulblets and soluble carbohydrates content in lily ‘Arabian Red’. Journal of Ornamental Plants, 5 (1): 7-13.‏

Howlader, J., Robin, A.H.K., Natarajan, S., Biswas, M.K., Sumi, K.R., Song, C.Y. and Nou, I.S. 2020. Transcriptome analysis by RnA–Seq reveals genes related to plant height in two sets of parent-hybrid combinations in Easter lily (Lilium longiflorum). Scientific Reports, 10 (1): 1-15.

Huang, H., Ge, Z., Limwachiranon, J., Li, L., Li, W. and Luo, Z. 2017. UV-C treatment affects browning and starch metabolism of minimally processed lily bulb. Postharvest Biology and Technology, 128: 105-111.‏

Li, J.W., Zhang, X.C., Wang, M.R., Bi, W.L., Faisal, M., da Silva, J.A.T. and Wang, Q.C. 2019. Development, progress and future prospects in cryobiotechnology of Lilium spp. Plant Methods, 15 (1): 1-12.‏

Lyngdoh, A., Gupta, Y.C., Dhiman, S.R., Dilta, B.S. and Kashyap, B. 2015. Effect of substrates on the propagation of hybrid lilies through scaling. Journal of Hill Agriculture, 6 (2): 158-162.‏

Marinangeli, P.A., Hernández, L.F., Pellegrini, C.P. and Curvetto, N.R. 2003.  Bulblet differentiation after scale propagation of Lilium longiflorum. Journal of the American Society for Horticultural Science, 128 (3): 324-329.‏

Masoodi, N.H. and Nayeem, S. 2018. Effect growth regulators and propagation media on the propagation of hybrid lilies through scaling. International Journal of Agriculture Sciences, 10 (9): 5924-5927.

Matsuo, E., Nonaka, A. and Arisumi, K. 1987. Some factors influencing the type of leaf development (plant type) of scale bulblets of Easter lily, Lilium longiflorum. Bul. Fac. Agr. Kagoshima University, Japan.

Memar Moshrefi, M., Moeini, A. and Tavasolian, E. 2004. Effects of plant growth regulators, and scale on propagation of Lilium ledebourii (Boiss). Iranian Journal of Field Crop Science, 35 (4): 1033-1041.

Narendra, C., Sindhu, S.S., Ramesh, K., Saha, T.N., Raju, D.V.S., Ajay, A. and Sharma, R.R. 2018. Effect of growing media composition on growth, flowering and bulb production of LA hybrid (Red Alert) and Oriental (Avocado) group of Lilium under protected condition. Indian Journal of Agricultural Sciences, 88 (12): 1843-1847.

Niimi, Y. 1985. Factor affecting the regeneration and growth of bulblets in bulb- scale cultures of Lilium rubllum Baker. Journal of the Japanese Society for Horticultural Science, 54 (1): 82- 86.

Niimi, Y. 1995. In vitro propagation and post- in vitro establishment of bulblets of Lilium japonicum Thunb.  Journal of Japan Society Horticulture Science, 63 (4): 843-852.

Niimi, Y., Nakano, M. and Isogai, N. 1999. Effects of temperature and illuminating conditions on regeneration and development of bulblets in scale culture of seven Lilium spp. Journal of the Japanese Society for Horticultural Science, 68 (1): 28-34.

Nongdhar, I., Singh, D. and Fatmi, U. 2019. Response of growing media on growth and flower quality of Asiatic Lilium CV. Ercalano in shade net under prayagraj condition, Plant Archives, 19 (2): 540-542.

Park, N. 1994. Effect of temperature, scale position and growth regulators on the bulblet formation and growth during scale propagation of Lilium. In International Symposium on the Genus Lilium, 414: 257-262.

Rajera, S. and Sharma, P. 2017. Effect of different growing media on bulb production of LA hybrid. Chemical Science Review and Letters, 6: 1382-1387.‏

Ramos, M.Á.V., Chanamé, C.E.M. and Astro, E.R.V. 2020. Induction of lily (Lilium sp.) bulblets from scales, using auxins and cytokinins. Scientia Agropecuaria, 11 (1): 75-81.

Sharma, P., Sharma, Y.D., Dhiman, S.R. and Gupta, Y.C. 2007. Effect of growth regulators and growing media on propagation of Oriental lily hybrids through scaling. Journal of Ornamental Horticulture, 10 (3): 148-152.‏

Statistics portal. 2021. Available: https://www.statista.com/statistics/641926/total-area-used-for-production-of-lily-bulbs-in-the-netherlands/. Accessed on [2021-03-13].

Stimart, D.P. and Asher, P.D. 1978. Tissue culture of bulb scale sections for asexual propagation of Lilium longiflorum Thunb.‏ Journal American Society of Horticulture Science, 103:182-184.

Suh, J.K. and Lee, J.S. 1996. Bulblet formation and dormancy induction as influenced by temperature, growing media and light quality during scaling propagation of Lilium species. Acta Horticulturae, 414: 251-256.

Sun, H.M., Jia-Zi, K. and Wang-Chun, X. 2008. Effects of GA₃ and IBA as well as media on scale cutting propagation in Lilium cv. ‘Elite’. Scientia Silvae Sinicae, 44 (12): 62-67.

Sun, L.L., Sunç, X.M., Zhang, Z.W., Li, Y.F. and Luo, F.X. 2008. Effect of phytohormone on bulb scale cutting propagation of Hyacinthus orientalis. Agriculturae Boreali-occidentalis Sinica, 17 (3): 290-293.

Sun, H.M., Tao-Wen, L., Wang-Chun, X. and Li-Tian, L. 2009. Effects of sawdust, peat, GA₃ and IBA on scale cutting propagation in Lilium davidii var. Unicolor. Journal of Shenyang Agricultural University, 40 (2): 160-164.

Tang, N., Ju, X., Hu, Y., Jia, R. and Tang, D. 2020. Effects of temperature and plant growth regulators on the scale propagation of Lilium davidii var. Unicolor. HortScience, 55 (6): 870-875.‏

van Le, B., Nhut, D.T. and Van, K.T.T. 1999. Plant production via shoot regeneration from thin cell layer pseudo-bulblet expiants of Lilium longiflorum in vitro. Comptes Rendus de l'Académie des Sciences-Series III-Sciences de la Vie, 322 (4): 303-310.

van Tuyl, J.M. and Arens, P. 2012. Bulbous ornamentals I and II. Floriculture Ornamental Biotechnol, 6 (Special Issue 1): 1–160.

Xiaona, Y.U.A.N., Zhuo, C.H.E.N. and Guixia, J.I.A. 2013. Effects of plant growth regulators on scale cutting propagation and carbohydrate metabolism of lily (Lilium L.). Acta Agriculturae Boreali-Occidentalis Sinica, 5: 109-115.

Zhang, W., Song, L., da Silva J.A.T. and Sun, H. 2013. Effects of temperature, plant growth regulators and substrates and changes in carbohydrate content during bulblet formation by twin scale propagation in Hippeastrum vittatum ‘Red Lion’. Scientia Horticulturae, 160: 230-237.‏