ارزیابی رنگیزه‌های فتوسنتزی، شاخص‌های فلورسانس، تبالات گازی وبرخی ترکیبات فلاونوئیدی موثر در گیاه بنفشه سه‌رنگ (Viola tricolor L.)تحت تاثیر نانوذرات نقره‌زیستی

فیزیولوژی محیطی گیاهی

دوره 18، شماره 69، فروردین 1402، صفحه 80-98   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2022.690248

نویسندگان

عارفه حسنوند¹؛ سارا سعادتمند^{* 2}؛ حسین لاری یزدی³؛ علیرضا ایرانبخش⁴

¹گروه زیست شناسی، دانشگاه پیام نور، تهران، ایران

²گروه زیست شناسی ، واحد علوم وتحقیقات ،دانشگاه آزاداسلامی،تهران،ایران

³گروه زیست شناسی ، دانشکده علوم پایه ،واحد بروجرد ،دانشگاه آزاداسلامی، بروجرد،ایران

⁴گروه زیست شناسی ، ، واحد علوم وتحقیقات ،دانشگاه آزاداسلامی،تهران،ایران

چکیده

بنفشه سه رنگ با نام علمیViola tricolor L.  از خانواده بنفشه گان یاViolaceae  است. که به دلیل دارا بودن ترکیبات انتی‌اکسیدانی و دارویی مورد استفاده قرار می‌گیرد. به منظور بررسی اثر نانو‌ذرات نقره بر سیستم فتوسنتزی و تبالات گازی گیاه بنفشه سه رنگ آزمایشی در قالب طرح کاملاً تصادفی با چهار تکرار انجام شد. تیمارها شامل نانو ذرات نقره در سه سطح (10، 50 و 100 پی‌پی‌ام) و یک شاهد (آب مقطر) بود. نتایج نشان داد محتوای نسبی آب، هدایت روزنه­ای، غلظت کلروفیل­های a و کل، فلورسانس بیشینه، کربوهیدرات محلول و فعالیت آنتی‌اکسیدانی در گیاهان تیمار شده نسبت به شاهد بیشتر شد. هدایت روزنه برگ در غلظت 50 میلی‌گرم بر لیتر نسبت به سطوح دیگر افزایش معنی‌دارنشان داد. بیشینه مقدار کلروفیل b در غلظت 10 پی‌‌پی‌ام مشاهده شد. همچنین نمونه­های گیاهی تیمار شده با غلظت 50 میلی­گرم در لیتر نانو ذرات نقره بیشترین مقدار کلروفیل a را داشتند. کارتنوئیدهادر تیمار گیاهان با نانونقره افزایش معنی‌داری نسبت به شاهد نشان داد. میزان CO₂ زیر اتاقک روزنه و کربوهیدرات نا محلول در شاهد نسبت به سایر تیمارها بیشتر بود. برخی ترکیبات فلاونوئیدی موثردر گیاه اندازه گیری شد،‌‌‌ از جمله روتین، کوئرستین و آپیژنین که به ترتیب  درتیمار 10، 50 و 10 پی‌پی‌ام نانوذرات نقره نسبت به شاهد افزایش معنی‌دار نشان دادند. نتایج این مطالعه نشان داد که نانوذرات نقره فعالیت آنتی اکسیدانی را تحریک کرده و متابولیت های ثانویه (مقدار فلاونوئیدها) بنفشه سه رنگ را افزایش می‌دهد.

کلیدواژه‌ها

روتین؛ کارتنوئید؛ کلروفیل؛ فعالیت آنتی اکسیدانی؛ هدایت روزنه‌ای

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