Features of the Biochemical Parameters of a Pendant Frog Blood in the Kazan City Lakes

Journal of Chemical Health Risks

دوره 12، شماره 4، اسفند 2022، صفحه 685-689   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.22034/jchr.2022.690772

نویسندگان

Nail Gosmanovich Nazarov^{* 1}؛ Renat Irekovich Zamaletdinov¹؛ Anton Olegovich Svinin²؛ Vladimir Vasilievich Zobov^{1، 3}

¹Institute of Management Economics and Finance, Kazan Federal University, Kazan, Russia

²Mari State University, 424000, Yoshkar-Ola, Russia

³A.E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center, Russian Academy of Sciences, Russia

چکیده

This esearch was performed aimed to study the biochemical parameters of the pond frog (Pelophylax lessonae) blood, from the populations inhabiting three biotopes of the Kazan city. Individuals of the pond frog Pelophylax lessonae were caught in July 2018 in three biotopes: a secondary reservoir near the Victory Park in Kazan, the Maloye Glubokoe karst lake and the Krugloye lake, which is a conditionally control area (a reserve). Determination of blood biochemical parameters included the analysis of glucose, lactate, total protein and hemoglobin. Two indicators were studied among the hematological signs: the content of erythrocytes and leukocytes. A lower content of glucose, total protein and an increased level of lactate were found in pond frogs from the population of the city center (Victory Park) in comparison with the conditionally control area and the "green zone" of the city. There was low hemoglobin content among the frogs from urban areas. The individuals from the P. lessonae population living in the Victory Park reservoir (urban population) showed a state of hypoglycemia, an increased blood lactate content, total protein, and hemoglobin decrease, and the increase of specific number of erythrocytes, which may indicate the conditions of hypoxia caused by high level of pollution of urban habitats and eutrophication of water bodies. Probably, various adaptive reactions are observed in anthropogenically disturbed amphibian habitats, consisting in erythrocyte number increase in response to the hemoglobin level decrease and also the change in the metabolic process intensity.

کلیدواژه‌ها

Pond frog؛ Pelophylax lessonae؛ Blood biochemistry؛ Hematology؛ Urban ecosystems

مراجع

1. Schwartz S.S., Smirnov V.S., Dobrinskii L.N., 1968. The method of morphophysiological indicators in the ecology of terrestrial vertebrates. Tr Inst Ekol Rast Zhivot. 58, 388.
2. Tsiperson V.P., Soloviev M.Y., 1997. The impact of chronic radioactive stress on the immuno-physiological condition of small mammals. Science of the total environment. 203(2), 105-113.
3. Vershinin V.L., 2004. The hemopoiesis of tailless amphibians - the specificity of the adaptation of the species in modern ecosystems. Zoologicheskiy Zhurnal. 83(11), 1367–1374.
4. Zhukova T.I., 1987. Changes in hematological indices of the lake frog in connection with dwelling in reservoirs polluted by pesticides. Russian Journal of Ecology. 2, 54–59.
5. Romanova E.B., Egorikhina, M.N., 2006. Changes in hematological parameters of Rana frogs in a transformed urban environment. Russian Journal of Ecology. 37(3), 188-192.
6. Zamaletdinov R.I., 2003. Ecology of amphibians in a big city (by the example of Kazan). Cand. Dissertation thesis. Kazan. pp.24.
7. Nikolaev V.Yu., 2016. Immunohematological characteristics of amphibians and reptiles of the Upper and Middle Volga region in the autecological aspect. Cand. Dissertation thesis. Nizhny Novgorod. pp.23.
8. Baraquet M., Grenat P.R., Salas N.E., Martino A.L., 2013. Intraspecific variation in erythrocyte sizes among populations of Hypsiboas cordobae (Anura: Hylidae). Acta Herpetologica. 8(2), 93–97.
9. Baraquet M., Salas N.E., Martino A.L., 2013. C-banding patterns and meiotic behavior in Hypsiboas pulchellus and H. cordobae (Anura, Hylidae). BAG. Journal of Basic and Applied Genetics. 24(1), 32-39.
10. Barrio A., 1965. Las subespecies de Hyla pulchella Duméril y Bibron (Anura, Hylidae). Physis. 25(69), 115-128.
11. Davis A.K., Maney D.L., Maerz J.C., 2008. The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology. 22(5), 760-772.
12. Breuner C.W., Delehanty B., Boonstra R., 2013. Evaluating stress in natural populations of vertebrates: total CORT is not good enough. Functional Ecology. 27(1), 24-36.
13. Zhelev Z.M., Popgeorgiev G.S., Mehterov N.H., 2015. Haematological parameters of Pelophylax ridibundus (Amphibia: Ranidae) from the region of the lead and zinc plant “Kardzhali”(south Bulgaria) and their use in the environmental quality assessment. Acta Zoologica Bulgarica. 67(2), 271-282.
14. Zhelev Z.M., Popgeorgiev G.S., Angelov M.V., 2013. Investigating the changes in the morphological content of the blood of Pelophylax ridibundus (Amphibia: Ranidae) as a result of anthropogenic pollution and its use as an environmental bioindicator. Acta Zoologica Bulgarica. 65(2), 187-196.
15. Zhelev Z.M., Popgeorgiev G.S., Georgieva Z.K., 2014. Fluctuating asymmetry in the populations of Pelophylax ridibundus and Pseudepidalea viridis (Amphibia: Anura) in the region of the lead and zinc plant “Kardzhali”(South Bulgaria). Acta Zoologica Bulgarica. 66(1), 83-87.
16. Alekin O.A., 1970. Basics of hydrochemistry (Leningrad: Gidrometeoizdat). pp.444.
17. Romanenko V.D., Oksiyuk O.A., Zhukinsky V.N., Stolberg F.V., Lavrik V.I., 1990. Ecological Assessment of the Impact of Hydrotechnical Construction on Water Bodies. pp.255.
18. Vershinin V.L., 1997. Ecological features of amphibian populations in urbanized areas. Manuscript. Doctoral thesis. Yekaterinburg.pp. 47.
19. Hamer A.J., McDonnell M.J., 2008. Amphibian ecology and conservation in the urbanising world: a review. Biological Conservation. 141(10), 2432-2449.
20. Vershinin V.L., Vershinina S.D., Berzin D.L., Zmeeva D.V., Kinev A.V., 2015. Long-term observation of amphibian populations inhabiting urban and forested areas in Yekaterinburg, Russia. Scientific Data. 2(1), 1-11.